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Literature summary for 1.13.11.52 extracted from

  • Pantouris, G.; Serys, M.; Yuasa, H.J.; Ball, H.J.; Mowat, C.G.
    Human indoleamine 2,3-dioxygenase-2 has substrate specificity and inhibition characteristics distinct from those of indoleamine 2,3-dioxygenase-1 (2014), Amino Acids, 46, 2155-2163 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid required Homo sapiens
methylene blue required Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene IDO1, recombinant expression His-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens
gene IDO2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of a IDO2 construct encoding an N-terminal truncated version of the predicted protein sequence consisting of residues 14-420 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-methyl-D-tryptophan competitive inhibition of IDO2, antitumour activity, selectivity of 1-methyltryptophan inhibition of IDO1 and IDO2 Homo sapiens
1-methyl-L-tryptophan competitive inhibition of IDO1, antitumour activity, selectivity of 1-methyl L-tryptophan inhibition of IDO1 and IDO2; competitive inhibition of IDO2, antitumour activity, selectivity of 1-methyltryptophan inhibition of IDO1 and IDO2 Homo sapiens
D-tryptophan low inhibition Homo sapiens
L-tryptophan
-
Homo sapiens
additional information no inhibition of IDO1 by 1-methy-D-tryptophan; no inhibition of IDO1 by L-tryptophan Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Homo sapiens
0.006
-
5-fluoro-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.017
-
5-hydroxy-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.0209
-
L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.07
-
1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.098
-
5-methyl-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.296
-
D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.547
-
5-methoxy-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.66
-
1-methyl-D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.696
-
1-methyl-L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.747
-
1-methyl-D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.57
-
5-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.768
-
5-fluoro-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
3.457
-
6-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
3.609
-
D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
6.809
-
L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + O2 Homo sapiens
-
N-formyl-L-kynurenine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14902
-
-
Homo sapiens Q6ZQW0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged IDO1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens
recombinant His-tagged IDO2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-D-tryptophan + O2
-
Homo sapiens N-methyl-N-formyl-D-kynurenine
-
?
1-methyl-L-tryptophan + O2
-
Homo sapiens N-methyl-N-formyl-L-kynurenine
-
?
5-fluoro-DL-tryptophan + O2
-
Homo sapiens 5-fluoro-N-formyl-DL-kynurenine
-
?
5-hydroxy-L-tryptophan + O2
-
Homo sapiens 5-hydroxy-N-formyl-L-kynurenine
-
?
5-methoxy-DL-tryptophan + O2
-
Homo sapiens 5-methoxy-N-formyl-DL-kynurenine
-
?
5-methyl-DL-tryptophan + O2
-
Homo sapiens 5-methyl-N-formyl-DL-kynurenine
-
?
5-methyl-DL-tryptophan + O2 high activity Homo sapiens 5-methyl-N-formyl-DL-kynurenine
-
?
6-methyl-DL-tryptophan + O2
-
Homo sapiens 6-methyl-N-formyl-DL-kynurenine
-
?
D-tryptophan + O2
-
Homo sapiens N-formyl-D-kynurenine
-
?
L-tryptophan + O2
-
Homo sapiens N-formyl-L-kynurenine
-
?
additional information substrate specificity, overview. Replacement of the 5-methoxy group by a methyl group has a negative influence in the binding of the molecule, decreasing its affinity threefold. 5-HO-L-Trp and melatonin are no substrates for IDO2 Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
IDO1
-
Homo sapiens
IDO2
-
Homo sapiens
indoleamine 2,3-dioxygenase-1
-
Homo sapiens
indoleamine 2,3-dioxygenase-2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0052
-
1-methyl-D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.0103
-
D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.011
-
1-methyl-L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.025
-
5-hydroxy-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.049
-
5-fluoro-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.062
-
1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.095
-
1-methyl-D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.103
-
L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.108
-
6-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.161
-
5-methoxy-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.36
-
5-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.76
-
5-fluoro-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
2.7
-
D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
2.97
-
L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
3.78
-
5-methyl-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Homo sapiens
7.5
-
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.018
-
1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.05
-
L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
0.3
-
1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.543
-
D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
3.3
-
1-methyl-D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO2 Homo sapiens
12
-
D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO2 Homo sapiens

General Information

General Information Comment Organism
metabolism indoleamine 2,3-dioxygenase-2 (IDO2) is one of three enzymes, alongside tryptophan 2,3-dioxygenase (EC 1.13.11.11) and indoleamine 2,3-dioxygenase (IDO1), that catalyse dioxygenation of L-tryptophan as the first step in the kynurenine pathway Homo sapiens
additional information human indoleamine 2,3-dioxygenase-2 substrate specificity and inhibition characteristics are distinct from those of indoleamine 2,3-dioxygenase-1 Homo sapiens
physiological function the enzyme IDO2 might have an immunomodulatory role unrelated to that of IDO1 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0028
-
D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.007
-
1-methyl-D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.015
-
L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.016
-
1-methyl-L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.028
-
5-fluoro-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.031
-
6-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.229
-
5-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
0.294
-
5-methoxy-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens