Cloned (Comment) | Organism |
---|---|
gene IDO1, semi-quantitative RT-PCR and quantitative real-time RT-PCR enzyme expression analysis in lipopolysaccgaride-stimulated THP-1 cells, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-methyl tryptophan | a tryptophan analogue. Galanal does not decrease the IkappaB-alpha expression in LPS-stimulated THP-1 cells | Homo sapiens | |
galanal | competitive inhibitor, IC50 value of 45 nM in the cell-based assay. galanal interfered with the transcriptional function of the nuclear factor-kappaB and the interferon-gamma signaling pathway. These effects of galanal are important for immune response. The inhibitory effect of galanal on IDO1 activity is stronger than that of 1-methyl tryptophan, a tryptophan analogue | Homo sapiens | |
additional information | suppressive effect of plant extracts or phytochemicals on IDO1 induction and activity. The methanol extracts of Myoga flower buds, which are traditional Japanese foods, and labdane-type diterpene galanal derived from Myoga flowers significantly suppress IDO1 activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.0526 | - |
L-tryptophan | pH 6.5, 37°C, recombinant His-tagged enzyme | Homo sapiens | |
0.0745 | - |
L-tryptophan | pH 6.5, 37°C, recombinant His-tagged enzyme in presence of inhibitor galanal | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | Homo sapiens | - |
N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | Homo sapiens | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14902 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
THP-1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | - |
Homo sapiens | N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | - |
Homo sapiens | N-formyl-L-kynurenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO1 | - |
Homo sapiens |
indoleamine 2,3-dioxygenase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0077 | - |
pH 6.5, 37°C, recombinant His-tagged enzyme | Homo sapiens | galanal |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | the methanol extracts of Myoga flower buds, which are traditional Japanese foods, and labdane-type diterpene galanal derived from Myoga flowers significantly suppress IDO1 activity | down |
Homo sapiens | suppressive effect of plant extracts or phytochemicals on IDO1 induction and activity | additional information |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the first and rate-limiting step in the degradation of L-tryptophan, has an important immunomodulatory function. The activity of IDO1 increases in various inflammatory diseases, including tumors, autoimmune diseases, and different kinds of inflammation | Homo sapiens |