Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Arabidopsis thaliana G protein beta | possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
transient expression of GFP-acireductone dioxygenase 1 in Arabidopsis thaliana protoplasts. The coding regions of ARD14 are cloned into pDEST17TM and pDEST15TM containing an N-terminal poly-His tag and an N-terminal GST tag, respectively, and are expressed in Escherichia coli strain Rosetta BL21 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | usage of three tDNA insertion mutant alleles, ard1-1 (SALK_119327), ard1-2 (GABI_595C04), and ard1-3 (SALK_034308). | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on, acireductone dioxygenase 1 is an active metalloenzyme, Fe2+ is active site bound | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | Arabidopsis thaliana | - |
4-(methylthio)-2-oxobutanoate + formate | - |
? | |
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | Arabidopsis thaliana Columbia-0 | - |
4-(methylthio)-2-oxobutanoate + formate | - |
? | |
additional information | Arabidopsis thaliana | acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview | ? | - |
? | |
additional information | Arabidopsis thaliana Columbia-0 | acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q0WSS4 | - |
- |
Arabidopsis thaliana Columbia-0 | Q0WSS4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged acireductone dioxygenase 1 and GST-tagged acireductone dioxygenase 1 by nickel affinity and glutathione affinity chromatographies, respectively | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | - |
Arabidopsis thaliana | 4-(methylthio)-2-oxobutanoate + formate | - |
? | |
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | - |
Arabidopsis thaliana Columbia-0 | 4-(methylthio)-2-oxobutanoate + formate | - |
? | |
additional information | acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview | Arabidopsis thaliana Columbia-0 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | homology modeling of acireductone dioxygenase 1, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
acireductone dioxygenase 1 | - |
Arabidopsis thaliana |
ARD1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
additional information | identification of potential effectors in the Arabidopsis thaliana G protein beta signaling pathway by an activation tagging approach to randomly create dominant suppressors of agb1-2, overview. Homology modeling of acireductone dioxygenase 1, overview | Arabidopsis thaliana |
physiological function | one function of acireductone dioxygenases is to operate in the methionine salvage pathway. Acireductone dioxygenase 1 physically interacts with Arabidopsis thaliana G protein beta, and acireductone dioxygenase enzymatic activity is stimulated by Arabidopsis thaliana G protein beta in vitro. Overexpression of acireductone dioxygenase 1 suppresses the 2-day-old etiolated phenotype of agb1-2. Acireductone dioxygenase 1 and Arabidopsis thaliana G protein beta both control embryonic hypocotyl length by modulating cell division. They also may contribute to the production of ethylene, a product of the methionine salvage pathway. Arabidopsis thaliana G protein beta regulates acireductone dioxygenase 1 activity to control cell division, possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview | Arabidopsis thaliana |