Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 0.05 mM, 70°C, 109.3% activity | Acidianus tengchongensis | |
dithiothreitol | 0.05 mM, 9.3% increase of activity | Acidianus tengchongensis | |
EDTA | 10 mM, 70°C, 108.8% activity | Acidianus tengchongensis | |
EDTA | 0.05 mM, 8.8% increase of activity | Acidianus tengchongensis | |
glutathione | 0.05 mM, 70°C, 110% activity | Acidianus tengchongensis | |
GSH | 0.05 mM, 10.8% increase of activity | Acidianus tengchongensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli HB101 | Acidianus tengchongensis |
overexpression of wild-type and mutant enzymes in Escherichia coli | Acidianus tengchongensis |
Protein Variants | Comment | Organism |
---|---|---|
C101S | mutant with reduced activity | Acidianus tengchongensis |
C101S | 98.4% loss of activity | Acidianus tengchongensis |
C104S | mutant with reduced activity | Acidianus tengchongensis |
C104S | 99.2% loss of activity | Acidianus tengchongensis |
C31A | complete loss of activity | Acidianus tengchongensis |
C31A | mutant with reduced activity | Acidianus tengchongensis |
C31S | complete loss of activity | Acidianus tengchongensis |
C31S | mutant with reduced activity | Acidianus tengchongensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM,89.2% inhibition | Acidianus tengchongensis | |
CoCl2 | 1 mM, 70°C, 10.8% activity | Acidianus tengchongensis | |
Cu2+ | 1 mM, 99.3% inhibition | Acidianus tengchongensis | |
CuCl2 | 1 mM, 70°C, 0.7% activity | Acidianus tengchongensis | |
Mg2+ | 1 mM, 22.8% inhibition | Acidianus tengchongensis | |
MgCl2 | 1 mM, 70°C, 77.2% activity | Acidianus tengchongensis | |
Mn2+ | 1 mM, 65.5% inhibition | Acidianus tengchongensis | |
MnCl2 | 1 mM, 70°C, 34.5% activity | Acidianus tengchongensis | |
N-ethylmaleimide | 0.1-1 mM, 70°C, 0.02-0.03% activity | Acidianus tengchongensis | |
NEM | 0.1 mM, complete | Acidianus tengchongensis | |
Ni2+ | 1 mM, 87.5% inhibition | Acidianus tengchongensis | |
NiCl2 | 1 mM, 70°C, 12.5% activity | Acidianus tengchongensis | |
Zn2+ | 1 mM, 70°C, 27% activity; 1 mM, 73% inhibition | Acidianus tengchongensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Acidianus tengchongensis | 5737 | - |
cytoplasm | also associated with the cytoplasmic membrane | Acidianus tengchongensis | 5737 | - |
cytoplasmic membrane | membrane-associated enzyme activity is colocalized with the activities of sulfite:acceptor oxidoreductase and thiosulfate:acceptor oxidoreductase to catalyze sulfur oxidation | Acidianus tengchongensis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidianus tengchongensis | - |
- |
- |
Acidianus tengchongensis S5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidianus tengchongensis |
Superdex 200 gel-filtration | Acidianus tengchongensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
mutant C104S, formation of sulfite plus thiosulfate, 70°C | Acidianus tengchongensis |
0.074 | - |
wild type in cellular lysate, formation of sulfite plus thiosulfate, 70°C | Acidianus tengchongensis |
0.08 | - |
mutant C101S, formation of sulfite plus thiosulfate, 70°C | Acidianus tengchongensis |
0.1078 | - |
wild type in supernatant, formation of sulfite plus thiosulfate, 70°C | Acidianus tengchongensis |
0.476 | - |
wild type in pellet, formation of sulfite plus thiosulfate, 70°C | Acidianus tengchongensis |
4.85 | - |
wild type, formation of sulfite plus thiosulfate, 70°C | Acidianus tengchongensis |
Storage Stability | Organism |
---|---|
-20°C | Acidianus tengchongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S + O2 + H2O | - |
Acidianus tengchongensis | HSO3- + H2S + H+ | - |
? | |
S + O2 + H2O | - |
Acidianus tengchongensis S5 | HSO3- + H2S + H+ | - |
? |