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show all sequences of 1.13.11.57

Crystallization and preliminary crystallographic analysis of gallate dioxygenase DesB from Sphingobium sp. SYK-6

Sugimoto, K.; Yamamoto, Y.; Antoni, S.; Senda, M.; Kasai, D.; Masai, E.; Fukuda, M.; Senda, T.; Acta Crystallogr. Sect. F F65, 1171-1174 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Sphingobium sp.
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 25% (w/v) PEG 8000, 0.1 M sodium acetate trihydrate, 0.1 M HEPES-NaOH pH 7.8, at 4°C
Sphingobium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
93800
-
calculated from amino acid sequence
Sphingobium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingobium sp.
-
formerly Sphingomonas paucimobilis strain SYK-6
-
Purification (Commentary)
Commentary
Organism
POROS PI column chromatography and POROS HQ column chromatography
Sphingobium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gallate + O2
-
713675
Sphingobium sp.
4-oxalomesaconate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
x-ray crystallography
Sphingobium sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Sphingobium sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 25% (w/v) PEG 8000, 0.1 M sodium acetate trihydrate, 0.1 M HEPES-NaOH pH 7.8, at 4°C
Sphingobium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
93800
-
calculated from amino acid sequence
Sphingobium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
POROS PI column chromatography and POROS HQ column chromatography
Sphingobium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gallate + O2
-
713675
Sphingobium sp.
4-oxalomesaconate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
x-ray crystallography
Sphingobium sp.
Other publictions for EC 1.13.11.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746263
Sugimoto
Molecular mechanism of strict ...
Sphingobium sp. SYK-6
PLoS ONE
9
e92249
2014
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-
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
716261
Nogales
Unravelling the gallic acid de ...
Pseudomonas putida, Pseudomonas putida KT 2240
Mol. Microbiol.
79
359-374
2011
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-
1
-
-
-
-
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1
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42
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1
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2
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1
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2
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713675
Sugimoto
Crystallization and preliminar ...
Sphingobium sp.
Acta Crystallogr. Sect. F
F65
1171-1174
2009
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-
1
1
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1
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2
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1
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1
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1
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1
1
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-
-
-
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715355
Kasai
Characterization of the gallat ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis SYK-6
J. Bacteriol.
187
5067-5074
2005
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-
1
-
-
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1
1
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1
3
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4
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1
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6
1
1
1
-
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1
1
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1
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1
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1
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1
3
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1
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6
1
1
1
-
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1
1
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1
1
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-
-
715480
Nogales
Molecular characterization of ...
Pseudomonas putida, Pseudomonas putida KT 2240
J. Biol. Chem.
280
35382-35390
2005
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1
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3
1
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1
3
-
-
44
-
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1
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2
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4
1
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1
1
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1
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3
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1
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1
3
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1
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2
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4
1
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1
1
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1
1
1
1
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