BRENDA - Enzyme Database show
show all sequences of 1.13.11.57

Characterization of the gallate dioxygenase gene: three distinct ring cleavage dioxygenases are involved in syringate degradation by Sphingomonas paucimobilis SYK-6

Kasai, D.; Masai, E.; Miyauchi, K.; Katayama, Y.; Fukuda, M.; J. Bacteriol. 187, 5067-5074 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Sphingomonas paucimobilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition at 0.5 mM, the oxygen consumption activity after EDTA treatment is completely recovered by addition of Fe2+ (119%)
Sphingomonas paucimobilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0669
-
gallate
in 50 mM GTA buffer (pH 8.5) consisting of 50 mM 3,3-dimethylglutarate, 50 mM Tris, and 50 mM 2-amino-2-methyl-1,3-propanediol, at 30°C
Sphingomonas paucimobilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
DesB requires Fe2+ for activity
Sphingomonas paucimobilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Sphingomonas paucimobilis
46843
-
2 * 46843, calculated from amino acid sequence
Sphingomonas paucimobilis
86000
-
gel filtration
Sphingomonas paucimobilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingomonas paucimobilis
Q5NTE5
-
-
Sphingomonas paucimobilis SYK-6
Q5NTE5
-
-
Purification (Commentary)
Commentary
Organism
POROS polyethyleneimine column chromatography
Sphingomonas paucimobilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gallate + O2
-
715355
Sphingomonas paucimobilis
4-oxalomesaconate
-
-
-
?
gallate + O2
-
715355
Sphingomonas paucimobilis SYK-6
4-oxalomesaconate
-
-
-
?
additional information
no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate
715355
Sphingomonas paucimobilis
?
-
-
-
-
additional information
no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate
715355
Sphingomonas paucimobilis SYK-6
?
-
-
-
-
pyrogallol + O2
-
715355
Sphingomonas paucimobilis
?
-
-
-
?
pyrogallol + O2
-
715355
Sphingomonas paucimobilis SYK-6
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE; 2 * 46843, calculated from amino acid sequence
Sphingomonas paucimobilis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Sphingomonas paucimobilis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
40
-
Sphingomonas paucimobilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Sphingomonas paucimobilis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Sphingomonas paucimobilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Sphingomonas paucimobilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition at 0.5 mM, the oxygen consumption activity after EDTA treatment is completely recovered by addition of Fe2+ (119%)
Sphingomonas paucimobilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0669
-
gallate
in 50 mM GTA buffer (pH 8.5) consisting of 50 mM 3,3-dimethylglutarate, 50 mM Tris, and 50 mM 2-amino-2-methyl-1,3-propanediol, at 30°C
Sphingomonas paucimobilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
DesB requires Fe2+ for activity
Sphingomonas paucimobilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Sphingomonas paucimobilis
46843
-
2 * 46843, calculated from amino acid sequence
Sphingomonas paucimobilis
86000
-
gel filtration
Sphingomonas paucimobilis
Purification (Commentary) (protein specific)
Commentary
Organism
POROS polyethyleneimine column chromatography
Sphingomonas paucimobilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gallate + O2
-
715355
Sphingomonas paucimobilis
4-oxalomesaconate
-
-
-
?
gallate + O2
-
715355
Sphingomonas paucimobilis SYK-6
4-oxalomesaconate
-
-
-
?
additional information
no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate
715355
Sphingomonas paucimobilis
?
-
-
-
-
additional information
no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate
715355
Sphingomonas paucimobilis SYK-6
?
-
-
-
-
pyrogallol + O2
-
715355
Sphingomonas paucimobilis
?
-
-
-
?
pyrogallol + O2
-
715355
Sphingomonas paucimobilis SYK-6
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE; 2 * 46843, calculated from amino acid sequence
Sphingomonas paucimobilis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Sphingomonas paucimobilis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
40
-
Sphingomonas paucimobilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Sphingomonas paucimobilis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Sphingomonas paucimobilis
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in syringate catabolism, the pathway involving DesB plays an especially important role in the growth of Sphingomonas paucimobilis SYK-6 on syringate, and DesB is involved in gallate degradation
Sphingomonas paucimobilis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in syringate catabolism, the pathway involving DesB plays an especially important role in the growth of Sphingomonas paucimobilis SYK-6 on syringate, and DesB is involved in gallate degradation
Sphingomonas paucimobilis
Other publictions for EC 1.13.11.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746263
Sugimoto
Molecular mechanism of strict ...
Sphingobium sp. SYK-6
PLoS ONE
9
e92249
2014
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
716261
Nogales
Unravelling the gallic acid de ...
Pseudomonas putida, Pseudomonas putida KT 2240
Mol. Microbiol.
79
359-374
2011
-
-
1
-
-
-
-
-
-
-
1
-
-
42
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713675
Sugimoto
Crystallization and preliminar ...
Sphingobium sp.
Acta Crystallogr. Sect. F
F65
1171-1174
2009
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715355
Kasai
Characterization of the gallat ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis SYK-6
J. Bacteriol.
187
5067-5074
2005
-
-
1
-
-
-
1
1
-
1
3
-
-
4
-
-
1
-
-
-
-
-
6
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
1
3
-
-
-
-
1
-
-
-
-
6
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
715480
Nogales
Molecular characterization of ...
Pseudomonas putida, Pseudomonas putida KT 2240
J. Biol. Chem.
280
35382-35390
2005
-
-
1
-
-
-
3
1
-
1
3
-
-
44
-
-
1
-
-
-
2
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
1
-
1
3
-
-
-
-
1
-
-
2
-
4
1
-
-
-
-
1
1
-
-
1
1
1
1
-
-