Data extracted from this reference:
Cloned(Commentary)
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas putida
Inhibitors
2,2'-dipyridyl
2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely
Pseudomonas putida
EDTA
complete inhibition at 1 mM, the activity is only fully recovered with Fe2+
Pseudomonas putida
H2O2
hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate
Pseudomonas putida
KM Value [mM]
0.144
gallate
in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Metals/Ions
Fe2+
required for activity
Pseudomonas putida
Molecular Weight [Da]
47000
3 * 47000, SDS-PAGE
Pseudomonas putida
47644
3 * 47644, calculated from amino acid sequence
Pseudomonas putida
132000
molar mass obtained by sedimentation equilibrium
Pseudomonas putida
Organism
Pseudomonas putida KT 2240
Purification (Commentary)
ultracentrifugation and phenyl-Sepharose column chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg]
21.4
crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
42.7
after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Substrates and Products (Substrate)
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida
4-oxalomesaconate
?
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida KT 2240
4-oxalomesaconate
?
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida
?
?
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida KT 2240
?
?
Subunits
homotrimer
3 * 47000, SDS-PAGE
Pseudomonas putida
homotrimer
3 * 47644, calculated from amino acid sequence
Pseudomonas putida
Synonyms
pH Optimum
pH Range
Cloned(Commentary) (protein specific)
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas putida
Inhibitors (protein specific)
2,2'-dipyridyl
2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely
Pseudomonas putida
EDTA
complete inhibition at 1 mM, the activity is only fully recovered with Fe2+
Pseudomonas putida
H2O2
hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate
Pseudomonas putida
KM Value [mM] (protein specific)
0.144
gallate
in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Metals/Ions (protein specific)
Fe2+
required for activity
Pseudomonas putida
Molecular Weight [Da] (protein specific)
47000
3 * 47000, SDS-PAGE
Pseudomonas putida
47644
3 * 47644, calculated from amino acid sequence
Pseudomonas putida
132000
molar mass obtained by sedimentation equilibrium
Pseudomonas putida
Purification (Commentary) (protein specific)
ultracentrifugation and phenyl-Sepharose column chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
21.4
crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
42.7
after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida
4-oxalomesaconate
?
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida KT 2240
4-oxalomesaconate
?
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida
?
?
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida KT 2240
?
?
Subunits (protein specific)
homotrimer
3 * 47000, SDS-PAGE
Pseudomonas putida
homotrimer
3 * 47644, calculated from amino acid sequence
Pseudomonas putida
pH Optimum (protein specific)
pH Range (protein specific)
Expression
Pseudomonas putida
the expression of the galA gene is specifically induced by gallate
up
General Information
physiological function
the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440
Pseudomonas putida
General Information (protein specific)
physiological function
the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440
Pseudomonas putida
Expression (protein specific)
Pseudomonas putida
the expression of the galA gene is specifically induced by gallate
up
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Characterization of the gallat ...
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