Literature summary for 1.13.11.57 extracted from

Nogales, J.; Canales, A.; Jimenez-Barbero, J.; Garcia, J.L.; Diaz, E.
Molecular characterization of the gallate dioxygenase from Pseudomonas putida KT2440. The prototype of a new subgroup of extradiol dioxygenases (2005), J. Biol. Chem., 280, 35382-35390.

Search for more literature for 1.13.11.57

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
2,2'-dipyridyl	2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely	Pseudomonas putida
EDTA	complete inhibition at 1 mM, the activity is only fully recovered with Fe2+	Pseudomonas putida
H2O2	hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.144	-	gallate	in 100 mM Tris-HCl, pH 7.0, at 30°C	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	required for activity	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	3 * 47000, SDS-PAGE	Pseudomonas putida
47644	-	3 * 47644, calculated from amino acid sequence	Pseudomonas putida
132000	-	molar mass obtained by sedimentation equilibrium	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-
Pseudomonas putida KT 2240	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ultracentrifugation and phenyl-Sepharose column chromatography	Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
21.4	-	crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C	Pseudomonas putida
42.7	-	after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gallate + O2	the substrate specificity is very restricted toward gallate	Pseudomonas putida	4-oxalomesaconate	-	?
gallate + O2	the substrate specificity is very restricted toward gallate	Pseudomonas putida KT 2240	4-oxalomesaconate	-	?
additional information	no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate	Pseudomonas putida	?	-	?
additional information	no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate	Pseudomonas putida KT 2240	?	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 47000, SDS-PAGE	Pseudomonas putida
homotrimer	3 * 47644, calculated from amino acid sequence	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
GALA	-	Pseudomonas putida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Pseudomonas putida

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	-	Pseudomonas putida

Expression

Organism	Comment	Expression
Pseudomonas putida	the expression of the galA gene is specifically induced by gallate	up

General Information

General Information	Comment	Organism
physiological function	the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440	Pseudomonas putida

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Release 2023.1 (January 2023)