show 
Molecular characterization of the gallate dioxygenase from Pseudomonas putida KT2440. The prototype of a new subgroup of extradiol dioxygenases
Nogales, J.; Canales, A.; Jimenez-Barbero, J.; Garcia, J.L.; Diaz, E.; J. Biol. Chem. 280, 35382-35390 (2005)
Data extracted from this reference:
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely
Pseudomonas putida
EDTA
complete inhibition at 1 mM, the activity is only fully recovered with Fe2+
Pseudomonas putida
H2O2
hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.144
-
gallate
in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for activity
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
3 * 47000, SDS-PAGE
Pseudomonas putida
47644
-
3 * 47644, calculated from amino acid sequence
Pseudomonas putida
132000
-
molar mass obtained by sedimentation equilibrium
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-
Purification (Commentary)
Commentary
Organism
ultracentrifugation and phenyl-Sepharose column chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
21.4
-
crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
42.7
-
after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida
4-oxalomesaconate
-
-
-
?
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida KT 2240
4-oxalomesaconate
-
-
-
?
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida
?
-
-
-
-
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida KT 2240
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homotrimer
3 * 47000, SDS-PAGE; 3 * 47644, calculated from amino acid sequence
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas putida
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely
Pseudomonas putida
EDTA
complete inhibition at 1 mM, the activity is only fully recovered with Fe2+
Pseudomonas putida
H2O2
hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.144
-
gallate
in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for activity
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
3 * 47000, SDS-PAGE
Pseudomonas putida
47644
-
3 * 47644, calculated from amino acid sequence
Pseudomonas putida
132000
-
molar mass obtained by sedimentation equilibrium
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
ultracentrifugation and phenyl-Sepharose column chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
21.4
-
crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
42.7
-
after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida
4-oxalomesaconate
-
-
-
?
gallate + O2
the substrate specificity is very restricted toward gallate
715480
Pseudomonas putida KT 2240
4-oxalomesaconate
-
-
-
?
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida
?
-
-
-
-
additional information
no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
715480
Pseudomonas putida KT 2240
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
3 * 47000, SDS-PAGE; 3 * 47644, calculated from amino acid sequence
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas putida
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Pseudomonas putida
Expression
Organism
Commentary
Expression
Pseudomonas putida
the expression of the galA gene is specifically induced by gallate
up
General Information
General Information
Commentary
Organism
physiological function
the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440
Pseudomonas putida
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440
Pseudomonas putida
Expression (protein specific)
Organism
Commentary
Expression
Pseudomonas putida
the expression of the galA gene is specifically induced by gallate
up
Other publictions for EC 1.13.11.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746263
Sugimoto
Molecular mechanism of strict ...
Sphingobium sp. SYK-6
PLoS ONE
9
e92249
2014
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
1
-
-
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-
-
-
-
716261
Nogales
Unravelling the gallic acid de ...
Pseudomonas putida, Pseudomonas putida KT 2240
Mol. Microbiol.
79
359-374
2011
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-
1
-
-
-
-
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1
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-
42
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1
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2
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1
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1
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1
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2
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713675
Sugimoto
Crystallization and preliminar ...
Sphingobium sp.
Acta Crystallogr. Sect. F
F65
1171-1174
2009
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-
1
1
-
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-
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1
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-
2
-
-
1
-
-
-
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1
1
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1
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1
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1
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1
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-
1
1
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-
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-
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-
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-
715355
Kasai
Characterization of the gallat ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis SYK-6
J. Bacteriol.
187
5067-5074
2005
-
-
1
-
-
-
1
1
-
1
3
-
-
4
-
-
1
-
-
-
-
-
6
1
1
1
-
-
1
1
-
-
-
-
-
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-
1
-
-
-
-
-
1
-
1
-
1
3
-
-
-
-
1
-
-
-
-
6
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
715480
Nogales
Molecular characterization of ...
Pseudomonas putida, Pseudomonas putida KT 2240
J. Biol. Chem.
280
35382-35390
2005
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1
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-
-
3
1
-
1
3
-
-
44
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1
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2
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4
1
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1
1
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1
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3
-
1
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1
3
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1
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2
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4
1
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1
1
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1
1
1
1
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