Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely | Pseudomonas putida | |
EDTA | complete inhibition at 1 mM, the activity is only fully recovered with Fe2+ | Pseudomonas putida | |
H2O2 | hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.144 | - |
gallate | in 100 mM Tris-HCl, pH 7.0, at 30°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
3 * 47000, SDS-PAGE | Pseudomonas putida |
47644 | - |
3 * 47644, calculated from amino acid sequence | Pseudomonas putida |
132000 | - |
molar mass obtained by sedimentation equilibrium | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultracentrifugation and phenyl-Sepharose column chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.4 | - |
crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C | Pseudomonas putida |
42.7 | - |
after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gallate + O2 | the substrate specificity is very restricted toward gallate | Pseudomonas putida | 4-oxalomesaconate | - |
? | |
gallate + O2 | the substrate specificity is very restricted toward gallate | Pseudomonas putida KT 2240 | 4-oxalomesaconate | - |
? | |
additional information | no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate | Pseudomonas putida | ? | - |
? | |
additional information | no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate | Pseudomonas putida KT 2240 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 47000, SDS-PAGE | Pseudomonas putida |
homotrimer | 3 * 47644, calculated from amino acid sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
GALA | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Pseudomonas putida |
Organism | Comment | Expression |
---|---|---|
Pseudomonas putida | the expression of the galA gene is specifically induced by gallate | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440 | Pseudomonas putida |