BRENDA - Enzyme Database show
show all sequences of 1.13.11.6

The purification and some properties of 3-hydroxyanthranilate oxygenase from baboon liver

Savage, N.; Levy, P.R.; Int. J. Biochem. 6, 459-466 (1975)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
p-Chloromercuriphenyl sulfonic acid
-
Papio ursinus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
4-Propyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.011
-
4-ethyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.037
-
4-methyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.105
-
3-hydroxyanthranilic acid
-
Papio ursinus
0.615
-
O2
-
Papio ursinus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
requirement
Papio ursinus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
40000
gel filtration, SDS-PAGE
Papio ursinus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxyanthranilate + O2
Papio ursinus
enzyme occurs in the metabolic pathway of the conversion of tryptophan to nicotinic acid
2-amino-3-carboxymuconate semialdehyde
-
-
?
Organic Solvent Stability
Organic Solvent
Commentary
Organism
additional information
enzyme is not stable, when stored frozen in 20 mM Tris-maleate buffer, 1 mM dithiothreitol and 1 mM FeSO4 at pH 6.5
Papio ursinus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papio ursinus
-
baboon
-
Purification (Commentary)
Commentary
Organism
-
Papio ursinus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Papio ursinus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.8
-
-
Papio ursinus
Storage Stability
Storage Stability
Organism
4C, overnight, 75% loss of activity
Papio ursinus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxyanthranilate + O2
-
439370
Papio ursinus
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
3-hydroxyanthranilate + O2
enzyme occurs in the metabolic pathway of the conversion of tryptophan to nicotinic acid
439370
Papio ursinus
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
4-ethyl-3-hydroxyanthranilate + O2
-
439370
Papio ursinus
2-amino-3-carboxy-4-ethylmuconate semialdehyde
-
439370
Papio ursinus
?
4-methyl-3-hydroxyanthranilate + O2
-
439370
Papio ursinus
2-amino-3-carboxy-4-methylmuconate semialdehyde
-
439370
Papio ursinus
?
4-propyl-3-hydroxyanthranilic acid + O2
-
439370
Papio ursinus
2-amino-3-carboxy-4-propylmuconate semialdehyde
-
439370
Papio ursinus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 35000-40000, SDS-PAGE
Papio ursinus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Papio ursinus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.6
-
Papio ursinus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-Chloromercuriphenyl sulfonic acid
-
Papio ursinus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
4-Propyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.011
-
4-ethyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.037
-
4-methyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.105
-
3-hydroxyanthranilic acid
-
Papio ursinus
0.615
-
O2
-
Papio ursinus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
requirement
Papio ursinus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
40000
gel filtration, SDS-PAGE
Papio ursinus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxyanthranilate + O2
Papio ursinus
enzyme occurs in the metabolic pathway of the conversion of tryptophan to nicotinic acid
2-amino-3-carboxymuconate semialdehyde
-
-
?
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
additional information
enzyme is not stable, when stored frozen in 20 mM Tris-maleate buffer, 1 mM dithiothreitol and 1 mM FeSO4 at pH 6.5
Papio ursinus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Papio ursinus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Papio ursinus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.8
-
-
Papio ursinus
Storage Stability (protein specific)
Storage Stability
Organism
4C, overnight, 75% loss of activity
Papio ursinus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxyanthranilate + O2
-
439370
Papio ursinus
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
3-hydroxyanthranilate + O2
enzyme occurs in the metabolic pathway of the conversion of tryptophan to nicotinic acid
439370
Papio ursinus
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
4-ethyl-3-hydroxyanthranilate + O2
-
439370
Papio ursinus
2-amino-3-carboxy-4-ethylmuconate semialdehyde
-
439370
Papio ursinus
?
4-methyl-3-hydroxyanthranilate + O2
-
439370
Papio ursinus
2-amino-3-carboxy-4-methylmuconate semialdehyde
-
439370
Papio ursinus
?
4-propyl-3-hydroxyanthranilic acid + O2
-
439370
Papio ursinus
2-amino-3-carboxy-4-propylmuconate semialdehyde
-
439370
Papio ursinus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 35000-40000, SDS-PAGE
Papio ursinus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Papio ursinus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.6
-
Papio ursinus
Other publictions for EC 1.13.11.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741516
Pidugu
Crystal structures of human 3 ...
Homo sapiens
Acta Crystallogr. Sect. D
73
340-348
2017
-
2
1
1
-
-
1
-
-
2
-
1
-
2
-
-
1
1
-
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1
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2
1
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1
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1
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2
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
743276
Brkic
Human 3-hydroxyanthranilate 3 ...
Homo sapiens
Mol. Biosyst.
11
898-907
2015
-
1
-
-
3
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
1
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1
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-
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3
-
-
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1
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1
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-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723950
Chakraborty
Aromatic ring cleavage of 2-am ...
Pseudomonas pseudoalcaligenes
Angew. Chem. Int. Ed. Engl.
52
920-924
2013
-
-
-
-
-
-
-
-
-
1
-
1
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1
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1
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1
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1
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-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
696784
Dilovic
Crystal structure of bovine 3- ...
Bos taurus
Biopolymers
91
1189-1195
2009
-
-
-
1
-
-
-
-
-
-
1
1
-
4
-
-
1
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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1
1
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1
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2
-
-
1
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
675557
Yates Jennifer
4-chloro-3-hydroxyanthranilate ...
Cavia porcellus, Cavia porcellus Hartley
J. Neurotrauma
23
866-881
2006
-
-
-
-
-
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1
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-
11
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-
-
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-
-
-
-
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-
675782
Maharaj
Acetylsalicylic acid and aceta ...
Rattus norvegicus, Rattus norvegicus Wistar
Metab. Brain Dis.
21
189-199
2006
-
-
-
-
-
-
2
-
-
-
-
-
-
168
-
-
-
-
-
1
-
-
2
-
-
-
-
-
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2
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1
-
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2
-
-
-
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-
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-
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-
-
-
-
-
-
-
677020
Li
Crystal structure of 3-hydroxy ...
Saccharomyces cerevisiae
Protein Sci.
15
761-773
2006
-
-
1
1
-
-
-
-
-
2
-
1
-
1
-
-
1
-
-
-
-
-
1
-
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-
-
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-
-
1
-
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-
1
1
1
-
-
-
-
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-
2
-
1
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-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
672036
Colabroy
The mechanism of inactivation ...
Cupriavidus metallidurans
Biochemistry
44
7623-7631
2005
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
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1
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1
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1
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1
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1
-
-
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-
-
-
-
-
-
672037
Zhang
Structural studies on 3-hydrox ...
Cupriavidus metallidurans
Biochemistry
44
7632-7643
2005
-
-
-
1
3
-
1
4
-
1
-
-
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1
-
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1
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1
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-
-
-
4
-
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-
-
-
1
3
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-
1
-
4
-
1
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-
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
673130
Comai
The effect of age on the enzym ...
Rattus norvegicus
Clin. Chim. Acta
360
67-80
2005
-
-
-
-
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2
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2
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-
649382
Muraki
Prokaryotic homologs of the eu ...
Pseudomonas fluorescens, Pseudomonas fluorescens KU-7
Appl. Environ. Microbiol.
69
1564-1572
2003
-
-
1
-
3
-
13
-
-
1
2
2
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5
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1
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4
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1
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3
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13
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1
2
2
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1
-
4
-
-
-
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-
-
-
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-
658927
Nandi
Purification and inactivation ...
Bos taurus
Int. J. Biochem. Cell Biol.
35
1085-1097
2003
-
-
-
-
-
-
3
2
-
-
4
1
-
2
-
-
1
-
-
2
1
-
2
1
-
-
1
-
1
-
-
-
5
2
-
-
-
-
-
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-
-
-
3
5
2
-
-
4
1
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1
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2
1
-
2
1
-
-
1
-
1
-
-
2
-
-
-
-
-
-
439377
Calderone
Cloning of human 3-hydroxyanth ...
Homo sapiens
Biochim. Biophys. Acta
1596
283-292
2002
-
-
1
-
-
-
3
-
1
1
1
1
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3
-
-
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1
-
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2
-
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1
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1
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3
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1
1
1
1
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-
2
-
-
-
-
1
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-
-
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-
-
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-
439378
Agrawal
QSAR study on inhibition of br ...
Rattus norvegicus
Bioorg. Med. Chem.
9
3295-3299
2001
-
-
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-
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1
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2
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1
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1
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439373
Fornstedt-Wallin
3-Hydroxyanthranilic acid accu ...
Rattus norvegicus
Eur. J. Pharmacol.
386
15-24
1999
-
-
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1
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1
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1
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1
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3
-
-
1
-
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-
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-
-
-
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-
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-
-
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439376
Chiarugi
Effects of mitochondria and o- ...
Mus musculus
J. Neurochem.
72
1125-1132
1999
-
-
-
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1
-
1
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
1
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-
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-
-
-
439379
Kucharczyk
The yeast gene YJR025c encodes ...
Escherichia coli, Saccharomyces cerevisiae
FEBS Lett.
424
127-130
1998
-
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2
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2
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4
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2
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2
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439375
Cesura
Molecular characterization of ...
Rattus norvegicus
Adv. Exp. Med. Biol.
398
477-483
1996
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1
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1
1
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1
1
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1
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1
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1
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1
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2
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1
1
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1
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