BRENDA - Enzyme Database show
show all sequences of 1.13.11.6

Purification and inactivation of 3-hydroxyanthranilic acid 3,4-dioxygenase from beef liver

Nandi, D.; Lightcap, E.S.; Koo, Y.K.; Lu, X.; Quancard, J.; Silverman, R.B.; Int. J. Biochem. Cell Biol. 35, 1085-1097 (2003)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
6-chloro-3-hydroxyanthranilic acid
5-20 mM, loss of enzymatic activity as a function of the inhibitor concentration
Bos taurus
anthranilic acid
competitive inhibition
Bos taurus
quinolinic acid
competitive inhibition
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
16
19
3-Hydroxyanthranilate
-
Bos taurus
36
57
3-hydroxy-4-methylanthranilic acid
-
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32520
-
PAGE in nondenaturing conditions, pI 4.98 enzyme
Bos taurus
32570
-
PAGE in nondenaturing conditions, pI 5.6 enzyme
Bos taurus
33000
34000
gel filtration
Bos taurus
33000
-
SDS-PAGE, for the two active enzyme solutions obtained from hydroxyapatite column
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxyanthranilate + O2
Bos taurus
-
2-amino-3-carboxymuconate semialdehyde
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
succesive size exclusion and affinity columns
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
115.7
-
last purification step
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxy-4-methylanthranilic acid + O2
-
658927
Bos taurus
?
-
-
-
?
3-hydroxyanthranilate + O2
-
658927
Bos taurus
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000-34000, gel filtration
Bos taurus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
4
stable for a month, in Tris-maleate buffer, pH 6.5
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
7.4
-
Bos taurus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8
-
quinolinic acid
for the pI 5.6 enzyme
Bos taurus
2
-
anthranilic acid
for the pI 4.98 enzyme
Bos taurus
4.9
-
anthranilic acid
for the pI 4.98 enzyme
Bos taurus
6.5
-
anthranilic acid
for the pI 5.6 enzyme
Bos taurus
12
-
6-chloro-3-hydroxyanthranilic acid
-
Bos taurus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Bos taurus
chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
4.98
Bos taurus
chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
5.6
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
6-chloro-3-hydroxyanthranilic acid
5-20 mM, loss of enzymatic activity as a function of the inhibitor concentration
Bos taurus
anthranilic acid
competitive inhibition
Bos taurus
quinolinic acid
competitive inhibition
Bos taurus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8
-
quinolinic acid
for the pI 5.6 enzyme
Bos taurus
2
-
anthranilic acid
for the pI 4.98 enzyme
Bos taurus
4.9
-
anthranilic acid
for the pI 4.98 enzyme
Bos taurus
6.5
-
anthranilic acid
for the pI 5.6 enzyme
Bos taurus
12
-
6-chloro-3-hydroxyanthranilic acid
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
16
19
3-Hydroxyanthranilate
-
Bos taurus
36
57
3-hydroxy-4-methylanthranilic acid
-
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32520
-
PAGE in nondenaturing conditions, pI 4.98 enzyme
Bos taurus
32570
-
PAGE in nondenaturing conditions, pI 5.6 enzyme
Bos taurus
33000
34000
gel filtration
Bos taurus
33000
-
SDS-PAGE, for the two active enzyme solutions obtained from hydroxyapatite column
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxyanthranilate + O2
Bos taurus
-
2-amino-3-carboxymuconate semialdehyde
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
succesive size exclusion and affinity columns
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
115.7
-
last purification step
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxy-4-methylanthranilic acid + O2
-
658927
Bos taurus
?
-
-
-
?
3-hydroxyanthranilate + O2
-
658927
Bos taurus
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000-34000, gel filtration
Bos taurus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
4
stable for a month, in Tris-maleate buffer, pH 6.5
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
7.4
-
Bos taurus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Bos taurus
chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
4.98
Bos taurus
chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
5.6
Other publictions for EC 1.13.11.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741516
Pidugu
Crystal structures of human 3 ...
Homo sapiens
Acta Crystallogr. Sect. D
73
340-348
2017
-
2
1
1
-
-
1
-
-
2
-
1
-
2
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
1
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1
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
743276
Brkic
Human 3-hydroxyanthranilate 3 ...
Homo sapiens
Mol. Biosyst.
11
898-907
2015
-
1
-
-
3
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
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1
-
-
-
3
-
-
-
-
-
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1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723950
Chakraborty
Aromatic ring cleavage of 2-am ...
Pseudomonas pseudoalcaligenes
Angew. Chem. Int. Ed. Engl.
52
920-924
2013
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
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1
-
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1
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1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
696784
Dilovic
Crystal structure of bovine 3- ...
Bos taurus
Biopolymers
91
1189-1195
2009
-
-
-
1
-
-
-
-
-
-
1
1
-
4
-
-
1
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675557
Yates Jennifer
4-chloro-3-hydroxyanthranilate ...
Cavia porcellus, Cavia porcellus Hartley
J. Neurotrauma
23
866-881
2006
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
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-
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-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675782
Maharaj
Acetylsalicylic acid and aceta ...
Rattus norvegicus, Rattus norvegicus Wistar
Metab. Brain Dis.
21
189-199
2006
-
-
-
-
-
-
2
-
-
-
-
-
-
171
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
2
-
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-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677020
Li
Crystal structure of 3-hydroxy ...
Saccharomyces cerevisiae
Protein Sci.
15
761-773
2006
-
-
1
1
-
-
-
-
-
2
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672036
Colabroy
The mechanism of inactivation ...
Cupriavidus metallidurans
Biochemistry
44
7623-7631
2005
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
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-
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-
1
-
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-
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-
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-
-
1
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-
-
-
-
-
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-
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672037
Zhang
Structural studies on 3-hydrox ...
Cupriavidus metallidurans
Biochemistry
44
7632-7643
2005
-
-
-
1
3
-
1
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
1
-
4
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
673130
Comai
The effect of age on the enzym ...
Rattus norvegicus
Clin. Chim. Acta
360
67-80
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
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-
2
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2
-
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-
-
-
-
-
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-
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-
-
-
-
-
-
-
649382
Muraki
Prokaryotic homologs of the eu ...
Pseudomonas fluorescens, Pseudomonas fluorescens KU-7
Appl. Environ. Microbiol.
69
1564-1572
2003
-
-
1
-
3
-
13
-
-
1
2
2
-
5
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
13
-
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-
1
2
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658927
Nandi
Purification and inactivation ...
Bos taurus
Int. J. Biochem. Cell Biol.
35
1085-1097
2003
-
-
-
-
-
-
3
2
-
-
4
1
-
2
-
-
1
-
-
2
1
-
2
1
-
-
1
-
1
-
-
-
5
2
-
-
-
-
-
-
-
-
-
3
5
2
-
-
4
1
-
-
-
1
-
2
1
-
2
1
-
-
1
-
1
-
-
2
-
-
-
-
-
-
439377
Calderone
Cloning of human 3-hydroxyanth ...
Homo sapiens
Biochim. Biophys. Acta
1596
283-292
2002
-
-
1
-
-
-
3
-
1
1
1
1
-
3
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
439378
Agrawal
QSAR study on inhibition of br ...
Rattus norvegicus
Bioorg. Med. Chem.
9
3295-3299
2001
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
1
-
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439373
Fornstedt-Wallin
3-Hydroxyanthranilic acid accu ...
Rattus norvegicus
Eur. J. Pharmacol.
386
15-24
1999
-
-
-
-
-
-
1
-
-
-
-
-
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1
-
-
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-
-
3
-
-
1
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1
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3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439376
Chiarugi
Effects of mitochondria and o- ...
Mus musculus
J. Neurochem.
72
1125-1132
1999
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
1
-
-
-
1
-
1
1
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-
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-
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-
-
-
-
-
-
1
-
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1
-
1
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1
-
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1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439379
Kucharczyk
The yeast gene YJR025c encodes ...
Escherichia coli, Saccharomyces cerevisiae
FEBS Lett.
424
127-130
1998
-
-
2
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
2
-
2
-
-
-
-
-
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2
-
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2
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2
-
2
-
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-
-
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-
-
-
-
-
-
-
-
-
439375
Cesura
Molecular characterization of ...
Rattus norvegicus
Adv. Exp. Med. Biol.
398
477-483
1996
-
-
1
-
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1
1
-
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2
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1
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1
1
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-
-
1
-
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1
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1
-
1
-
-
2
-
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-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439374
Roberts
3-Hydroxyanthranilic acid oxyg ...
Rattus norvegicus
J. Neurosci.
15
1150-1161
1995
-
-
-
-
-
-
-
-
-
-
-
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