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Literature summary for 1.13.11.60 extracted from

  • Garscha, U.; Oliw, E.H.
    Critical amino acids for the 8(R)-dioxygenase activity of linoleate diol synthase. A comparison with cyclooxygenases (2008), FEBS Lett., 582, 3547-3551.
    View publication on PubMed
Search for more literature for 1.13.11.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Spodoptera frugiperda (Sf21) insect cells with native 8R-dioxygenase and hydroperoxide isomerase activities Gaeumannomyces graminis

Protein Variants

Protein Variants Comment Organism
H203Q weak activity Gaeumannomyces graminis
H379Q inactive mutant enzyme Gaeumannomyces graminis
K540L inactive mutant enzyme Gaeumannomyces graminis
K540Q inactive mutant enzyme Gaeumannomyces graminis
K540R inactive mutant enzyme Gaeumannomyces graminis
Y329F weak activity Gaeumannomyces graminis
Y329L inactive mutant enzyme Gaeumannomyces graminis
Y376F inactive mutant enzyme Gaeumannomyces graminis
Y378F inactive mutant enzyme Gaeumannomyces graminis
Y378S inactive mutant enzyme Gaeumannomyces graminis
Y531F inactive mutant enzyme Gaeumannomyces graminis

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis Q9UUS2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
-
 Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?

Synonyms

Synonyms Comment Organism
7,8-LDS bifunctional enzyme Gaeumannomyces graminis
7,8-linoleate diol synthase bifunctional enzyme Gaeumannomyces graminis
8R-dioxygenase
-
 Gaeumannomyces graminis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Gaeumannomyces graminis