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Literature summary for 1.13.11.61 extracted from

  • Kim, S.; Kim, T.; Kim, M.; Oh, D.
    Production of 11R-hydroxyeicosatetraenoic acid from arachidonic acid by Escherichia coli cells expressing arachidonate 11R-lipoxygenase from Nostoc sp. (2021), J. Am. Oil Chem. Soc., 2021, 1-9 .
No PubMed abstract available
Search for more literature for 1.13.11.61

Activating Compound

Activating Compound Comment Organism Structure
DMSO about 145% activity at 5% (v/v) Nostoc sp. PCC 7120 = FACHB-418
ethanol about 155% activity at 5% (v/v) Nostoc sp. PCC 7120 = FACHB-418
iso-propanol about 140% activity at 5% (v/v) Nostoc sp. PCC 7120 = FACHB-418
methanol about 130% activity at 5% (v/v) Nostoc sp. PCC 7120 = FACHB-418

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Nostoc sp. PCC 7120 = FACHB-418

Inhibitors

Inhibitors Comment Organism Structure
acetone about 60% residual activity at 2% (v/v) Nostoc sp. PCC 7120 = FACHB-418
butanol about 65% residual activity at 5% (v/v) Nostoc sp. PCC 7120 = FACHB-418
ethyl acetate about 70% residual activity at 5% (v/v) Nostoc sp. PCC 7120 = FACHB-418
hexane about 80% residual activity at 2% (v/v) Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 alpha-linolenic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
0.054
-
 gamma-linolenic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
0.404
-
 linoleic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography and Superdex 200 gel filtration Nostoc sp. PCC 7120 = FACHB-418

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
 native enzyme, with gamma-linolenic acid as substrate, at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
6.3
-
 native enzyme, with alpha-linolenic acid as substrate, at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
18.4
-
 native enzyme, with linoleic acid as substrate, at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenic acid + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 (9Z,12E,15Z)-9R-hydroxy-9,12,15-octadecatrienoic acid
-
 ?
gamma-linolenic acid + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 (6Z,10E,12Z)-9R-hydroxy-6,10,12-octadecatrienoic acid
-
 ?
linoleic acid + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 (10E,12Z)-9R-hydroxy-10,12-octadecadienoic acid
-
 ?

Synonyms

Synonyms Comment Organism
11R-LOX
-
 Nostoc sp. PCC 7120 = FACHB-418
9R-LOX
-
 Nostoc sp. PCC 7120 = FACHB-418
ARA 11RLOX
-
 Nostoc sp. PCC 7120 = FACHB-418
arachidonate 11R-lipoxygenase
-
 Nostoc sp. PCC 7120 = FACHB-418
LA 9RLOX
-
 Nostoc sp. PCC 7120 = FACHB-418
linoleate 11R-lipoxygenase
-
 Nostoc sp. PCC 7120 = FACHB-418

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Nostoc sp. PCC 7120 = FACHB-418

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 35 more than 60% activity between 15 and 35°C Nostoc sp. PCC 7120 = FACHB-418

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.097
-
 gamma-linolenic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
0.13
-
 alpha-linolenic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
5.12
-
 linoleic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Nostoc sp. PCC 7120 = FACHB-418

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 more than 50% activity between pH 6.5 and 8.5 Nostoc sp. PCC 7120 = FACHB-418

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
 gamma-linolenic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
2.4
-
 alpha-linolenic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418
12.7
-
 linoleic acid at pH 7.0 and 25°C Nostoc sp. PCC 7120 = FACHB-418