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Literature summary for 1.13.11.63 extracted from

  • Lindqvist, A.; Andersson, S.
    Biochemical properties of purified recombinant human beta-carotene 15,15'-monooxygenase (2002), J. Biol. Chem., 277, 23942-23948.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1-S-octyl-beta-D-thioglucopyranoside detergent required, maximal activity at 1% w/v Homo sapiens
octyl beta-glucoside detergent required Homo sapiens
SDS detergent required, low activation Homo sapiens
sodium cholate detergent required Homo sapiens
Triton X-100 detergent required Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression by an baculovirus/Spodoptera frugiperda 9 insect cell system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
Homo sapiens
PCMB
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0071
-
beta-carotene
-
Homo sapiens
0.03
-
beta-cryptoxanthin
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + O2 Homo sapiens the enzyme catalyzes the first step in the synthesis of vitamin A from dietary carotenoids. May also play a role in peripheral vitamin A synthesis from plasma-borne provitamin A carotenoids retinal
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAY6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
intestine high levels of BCO mRNA, highest level in jejunum Homo sapiens
-
kidney high levels of BCO mRNA Homo sapiens
-
liver high levels of BCO mRNA Homo sapiens
-
ovary low levels of BCO mRNA Homo sapiens
-
prostate gland low levels of BCO mRNA Homo sapiens
-
skeletal muscle low levels of BCO mRNA Homo sapiens
-
testis low levels of BCO mRNA Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2 the enzyme catalyzes the first step in the synthesis of vitamin A from dietary carotenoids. May also play a role in peripheral vitamin A synthesis from plasma-borne provitamin A carotenoids Homo sapiens retinal
-
?
beta-cryptoxanthin + O2
-
Homo sapiens ?
-
?
beta-cryptoxanthin + O2
-
Homo sapiens all-trans-retinal + ?
-
r

Subunits

Subunits Comment Organism
tetramer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
BCO
-
Homo sapiens
beta-carotene 15,15'-monooxygenase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
beta-carotene
-
Homo sapiens
0.668
-
beta-cryptoxanthin
-
Homo sapiens