Cloned (Comment) | Organism |
---|---|
expression of BCO1 with a C-terminal polyhistidine tag in Escherichia coli strain BL21-Gold(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0172 | - |
beta-carotene | recombinant His6-tagged enzyme, pH and temperature not specified in the publication | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63702 | - |
x * 63702, His-tagged enzyme, sequence calculation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | Homo sapiens | - |
2 all-trans-retinal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HAY6 | gene BCMO1; gene Bcmo1 | - |
Purification (Comment) | Organism |
---|---|
recombinant BCO1 with a C-terminal polyhistidine tag from Escherichia coli strain BL21-Gold(DE3) by cobalt affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0022 | - |
purified recombinant His6-tagged enzyme, pH and temperature not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-carotene + O2 | - |
Homo sapiens | 2 retinal | - |
? | |
beta-apo-8'-carotenal + O2 | - |
Homo sapiens | retinal + ? | - |
? | |
beta-carotene + O2 | - |
Homo sapiens | 2 all-trans-retinal | - |
? | |
beta-cryptoxanthin + O2 | - |
Homo sapiens | 2 retinal | - |
? | |
lycopene + O2 | - |
Homo sapiens | 2 acycloretinal | - |
? | |
additional information | shorter beta-apocarotenals, i.e. beta-apo-10-carotenal, beta-apo-12'-carotenal, and beta-apo-14'-carotenal, do not show Michaelis-Menten behavior under the conditions tested. No activity with lutein, zeaxanthin, and 9-cis-beta-carotene. Purified recombinant BCO1 cleaves beta-carotene solely at the central 15-15' bond | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63702, His-tagged enzyme, sequence calculation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
BCO1 | - |
Homo sapiens |
beta-carotene 15,15'-oxygenase | - |
Homo sapiens |