Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 1 mM, 98% loss of activity | Bradyrhizobium sp. | |
o-phenanthroline | 1 mM, complete loss of activity | Bradyrhizobium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions | Bradyrhizobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium sp. | D3WZ86 | - |
- |
Renatured (Comment) | Organism |
---|---|
ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions | Bradyrhizobium sp. |
Storage Stability | Organism |
---|---|
-20°C, 40% to 50% loss of original activity after 1 week of storage | Bradyrhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitro-6-oxohepta-2,4-dienedioate | - |
Bradyrhizobium sp. | 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite | 2nd step of reaction, spontaneous | ? | |
5-nitrosalicylate + O2 | oxidation catalyzed by cell extracts requires 0.97 mol O2/mol 5-nitrosalicylate | Bradyrhizobium sp. | 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite | overall reaction | ? | |
5-nitrosalicylate + O2 | - |
Bradyrhizobium sp. | 4-nitro-6-oxohepta-2,4-dienedioate | 1st step of reaction | ? | |
salicylate + O2 | - |
Bradyrhizobium sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5NSA dioxygenase | - |
Bradyrhizobium sp. |
naaB | - |
Bradyrhizobium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement of NADPH | Bradyrhizobium sp. |