Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingobium chlorophenolicum |
Crystallization (Comment) | Organism |
---|---|
generation of a homology model, based on zinc protein PDB entry 1ZSW, which predicts that the tertiary structure of the enzyme differs significantly from that of the extradiol dioxygenases, and that the residues ligating the Fe(II) are H11, H227, and E276 | Sphingobium chlorophenolicum |
Protein Variants | Comment | Organism |
---|---|---|
E276A | less than 6% of wild-type activity | Sphingobium chlorophenolicum |
H11A | less than 6% of wild-type activity | Sphingobium chlorophenolicum |
H159A | 67% of wild-type activity | Sphingobium chlorophenolicum |
H227A | less than 6% of wild-type activity | Sphingobium chlorophenolicum |
Y266F | about 6% of wild-type activity | Sphingobium chlorophenolicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the residues ligating the Fe(II) are H11, H227, and E276 | Sphingobium chlorophenolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | Q9ZBB0 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PcpA | - |
Sphingobium chlorophenolicum |