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Literature summary for 1.13.11.68 extracted from
- On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7 (2014), FEBS Lett., 588, 1802-1807 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ccd7, recombinant expression in Escherichia coli strain BL21(DE3) | Pisum sativum |
| gene ccd7, recombinant expression in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Pisum sativum | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Arabidopsis thaliana | |
| 0.0411 | - |
9-cis-beta-carotene | recombinant enzyme, pH not specified in the publication, 28°C | Arabidopsis thaliana |  |
| 0.0596 | - |
9-cis-beta-carotene | recombinant enzyme, pH not specified in the publication, 28°C | Pisum sativum | |
| 0.1487 | - |
9-cis-zeaxanthin | recombinant enzyme, pH not specified in the publication, 28°C | Arabidopsis thaliana | |
| 0.626 | - |
9-cis-zeaxanthin | recombinant enzyme, pH not specified in the publication, 28°C | Pisum sativum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9-cis-beta-carotene + O2 | Pisum sativum | - |
9-cis-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| 9-cis-beta-carotene + O2 | Arabidopsis thaliana | - |
9-cis-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| 9-cis-zeaxanthin + O2 | Pisum sativum | - |
9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone | - |
? | |
| 9-cis-zeaxanthin + O2 | Arabidopsis thaliana | - |
9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone | - |
? | |
| additional information | Arabidopsis thaliana | substrate- and stereospecificity of Arabidopsis thaliana carotenoid cleavage dioxygenase 7, overview | ? | - |
? | |
| additional information | Pisum sativum | substrate- and stereospecificity of Pisum sativum carotenoid cleavage dioxygenase 7, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q7XJM2 | - |
- |
| Pisum sativum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9'-cis-cryptoxanthin + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
| 9-cis-beta-carotene + O2 | - |
Pisum sativum | 9-cis-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| 9-cis-beta-carotene + O2 | - |
Arabidopsis thaliana | 9-cis-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| 9-cis-cryptoxanthin + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
| 9-cis-lutein + O2 | - |
Pisum sativum | 9-cis-3-hydroxy-epsilon-apo-10'-carotenal + 3-hydroxy-beta-ionone | - |
? | |
| 9-cis-lutein + O2 | - |
Arabidopsis thaliana | 9-cis-3-hydroxy-epsilon-apo-10'-carotenal + 3-hydroxy-beta-ionone | - |
? | |
| 9-cis-zeaxanthin + O2 | - |
Pisum sativum | 9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone | - |
? | |
| 9-cis-zeaxanthin + O2 | - |
Arabidopsis thaliana | 9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone | - |
? | |
| additional information | substrate- and stereospecificity of Arabidopsis thaliana carotenoid cleavage dioxygenase 7, overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | substrate- and stereospecificity of Pisum sativum carotenoid cleavage dioxygenase 7, overview | Pisum sativum | ? | - |
? | |
| additional information | enzyme CCD7 cleaves different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10'-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-beta-carotene. The enzyme has a higher affinity to 9-cis-beta-carotene than to 9-cis-zeaxanthin, but converts the latter with higher rate. No detectable conversion of 9-cis-violaxanthin, all-trans-lutein, -zeaxanthin or -cryptoxanthin | Pisum sativum | ? | - |
? | |
| additional information | enzyme CCD7 cleaves different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10'-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-beta-carotene. The enzyme has a higher affinity to 9-cis-beta-carotene than to 9-cis-zeaxanthin, but converts the latter with higher rate. No detectable conversion of 9-cis-violaxanthin, all-trans-lutein, -zeaxanthin or -cryptoxanthin | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carotenoid cleavage dioxygenase 7 | - |
Pisum sativum |
| carotenoid cleavage dioxygenase 7 | - |
Arabidopsis thaliana |
| CCD7 | - |
Pisum sativum |
| CCD7 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Pisum sativum |
| 28 | - |
assay at | Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-beta-carotene to form a supposedly 9-cis-configured beta-apo-10'-carotenal | Pisum sativum |
| physiological function | strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-beta-carotene to form a supposedly 9-cis-configured beta-apo-10'-carotenal | Arabidopsis thaliana |
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