Literature summary for 1.13.11.74 extracted from

Takenaka, S.; Murakami, S.; Shinke, R.; Hatakeyama, K.; Yukawa, H.; Aoki, K.
Novel genes encoding 2-aminophenol 1,6-dioxygenase from Pseudomonas species AP-3 growing on 2-aminophenol and catalytic properties of the purified enzyme (1997), J. Biol. Chem., 272, 14727-14732.

Search for more literature for 1.13.11.74

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism
1,2,4-Trihydroxybenzene	noncompetitive inhibition	Pseudomonas sp.
2,2'-dipyridyl	complete inhibition at 0.5 mM	Pseudomonas sp.
3-chlorocatechol	competitive inhibition	Pseudomonas sp.
3-fluorocatechol	noncompetitive inhibition	Pseudomonas sp.
3-methylcatechol	noncompetitive inhibition	Pseudomonas sp.
4-aminoresorcinol	noncompetitive inhibition	Pseudomonas sp.
4-Chlorocatechol	competitive inhibition	Pseudomonas sp.
4-methylcatechol	uncompetitive inhibition	Pseudomonas sp.
5,5'-dithiobis-(2-nitrobenzoic acid)	complete inhibition at 0.5 mM	Pseudomonas sp.
AgNO3	6% residual activity at 0.5 mM	Pseudomonas sp.
catechol	noncompetitive inhibition	Pseudomonas sp.
CuSO4	complete inhibition at 0.1 mM	Pseudomonas sp.
EDTA	complete inhibition at 0.5 mM	Pseudomonas sp.
FeCl3	complete inhibition at 0.5 mM	Pseudomonas sp.
FeSO4(NH4)2SO4	78% residual activity at 0.5 mM	Pseudomonas sp.
HgCl2	12% residual activity at 0.5 mM	Pseudomonas sp.
iodoacetate	40% residual activity at 0.5 mM	Pseudomonas sp.
K3Fe(CN)6	14% residual activity at 0.5 mM	Pseudomonas sp.
MgSO4	79% residual activity at 0.5 mM	Pseudomonas sp.
MnCl2	53% residual activity at 0.5 mM	Pseudomonas sp.
additional information	not inhibited by FeSO4	Pseudomonas sp.
N-ethylmaleimide	83% residual activity at 0.5 mM	Pseudomonas sp.
NaN3	complete inhibition at 0.5 mM	Pseudomonas sp.
o-phenanthroline	26% residual activity at 0.5 mM	Pseudomonas sp.
p-chloromercuribenzoic acid	65% residual activity at 0.5 mM	Pseudomonas sp.
pyrogallol	noncompetitive inhibition	Pseudomonas sp.
Tiron	complete inhibition at 0.5 mM	Pseudomonas sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0467	-	2-Aminophenol	pH and temperature not specified in the publication	Pseudomonas sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains 0.98 mol of Fe2+ per mol of protein	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	2 * 40000 + 2 * 32000, SDS-PAGE	Pseudomonas sp.
40000	-	2 * 40000 + 2 * 32000, SDS-PAGE	Pseudomonas sp.
140000	-	gel filtration	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-
Pseudomonas sp. Ap-3	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
acetone precipitation, DE52-cellulose column chromatography, DEAE-cellulofine A-500 column chromatography, and gel filtration	Pseudomonas sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:2-aminophenol-grown cell	-	Pseudomonas sp.	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2-amino-4,5-dimethylphenol + O2	1.3% activity compared to 2-aminophenol	Pseudomonas sp.	?	-	?
2-amino-4,5-dimethylphenol + O2	1.3% activity compared to 2-aminophenol	Pseudomonas sp. Ap-3	?	-	?
2-amino-4-chlorophenol + O2	6.8% activity compared to 2-aminophenol	Pseudomonas sp.	?	-	?
2-amino-4-chlorophenol + O2	6.8% activity compared to 2-aminophenol	Pseudomonas sp. Ap-3	?	-	?
2-amino-m-cresol + O2	4.4% activity compared to 2-aminophenol	Pseudomonas sp.	?	-	?
2-amino-p-cresol + O2	10% activity compared to 2-aminophenol	Pseudomonas sp.	?	-	?
2-amino-p-cresol + O2	10% activity compared to 2-aminophenol	Pseudomonas sp. Ap-3	?	-	?
2-aminophenol + O2	100% activity	Pseudomonas sp.	2-aminomuconate 6-semialdehyde	-	?
2-aminophenol + O2	100% activity	Pseudomonas sp. Ap-3	2-aminomuconate 6-semialdehyde	-	?
6-amino-m-cresol + O2	19.1% activity compared to 2-aminophenol	Pseudomonas sp.	?	-	?
additional information	no activity with phenol, o-, m-, or p-cresol, aniline, o-, m-, or p-aminobenzoic acid, o-, m-, or p-phenylenediamine, protocatechuic acid, 2,3-dihydroxybenzoic acid, 3-aminophenol, 4-aminophenol, 2-amino-4-isopropylphenol, 2-amino-tert-butylphenol, 3-aminophenazin-2-ol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 3-amino-4-hydroxybenzoic acid, or 4-amino-3-hydroxybenzoic acid, amidol and 3-hydroxyanthralinic acid	Pseudomonas sp.	?	-	?
additional information	no activity with phenol, o-, m-, or p-cresol, aniline, o-, m-, or p-aminobenzoic acid, o-, m-, or p-phenylenediamine, protocatechuic acid, 2,3-dihydroxybenzoic acid, 3-aminophenol, 4-aminophenol, 2-amino-4-isopropylphenol, 2-amino-tert-butylphenol, 3-aminophenazin-2-ol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 3-amino-4-hydroxybenzoic acid, or 4-amino-3-hydroxybenzoic acid, amidol and 3-hydroxyanthralinic acid	Pseudomonas sp. Ap-3	?	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	2 * 40000 + 2 * 32000, SDS-PAGE	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
amnA	subunit alpha	Pseudomonas sp.
amnB	subunit beta	Pseudomonas sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.0054	-	3-fluorocatechol	pH and temperature not specified in the publication	Pseudomonas sp.
0.0067	-	3-chlorocatechol	pH and temperature not specified in the publication	Pseudomonas sp.
0.0068	-	3-methylcatechol	pH and temperature not specified in the publication	Pseudomonas sp.
0.0084	-	pyrogallol	pH and temperature not specified in the publication	Pseudomonas sp.
0.0091	-	4-aminoresorcinol	pH and temperature not specified in the publication	Pseudomonas sp.
0.0095	-	4-methylcatechol	pH and temperature not specified in the publication	Pseudomonas sp.
0.0104	-	catechol	pH and temperature not specified in the publication	Pseudomonas sp.
0.0104	-	4-Chlorocatechol	pH and temperature not specified in the publication	Pseudomonas sp.
0.063	-	1,2,4-Trihydroxybenzene	pH and temperature not specified in the publication	Pseudomonas sp.