BRENDA - Enzyme Database
show all sequences of 1.13.11.76

A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli

Wu, J.F.; Sun, C.W.; Jiang, C.Y.; Liu, Z.P.; Liu, S.J.; Arch. Microbiol. 183, 1-8 (2005) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Comamonas testosteroni
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
2 mM, 79% residual activity
Comamonas testosteroni
Cd2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
Co2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
Cu2+
2 mM, 2.9% residual activity
Comamonas testosteroni
EDTA
2 mM, 50% residual activity
Comamonas testosteroni
Fe2+
2 mM, 57% residual activity. Not inhibitory in presence of ascorbate
Comamonas testosteroni
H2O2
2 mM, complete loss of activity
Comamonas testosteroni
Mg2+
2 mM, 91% residual activity
Comamonas testosteroni
Mn2+
2 mM, 31% residual activity
Comamonas testosteroni
Ni2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
Zn2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00077
-
2-amino-5-chlorophenol
pH 8.0, 22°C
Comamonas testosteroni
0.00089
-
2-Aminophenol
pH 8.0, 22°C
Comamonas testosteroni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE
Comamonas testosteroni
38000
-
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE
Comamonas testosteroni
130000
-
gel filtration
Comamonas testosteroni
Organism
Organism
UniProt
Commentary
Textmining
Comamonas testosteroni
Q6J1Z5
subunit alpha
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Comamonas testosteroni
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
29.7
-
pH 8.0, 22°C
Comamonas testosteroni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-amino-5-chlorophenol + O2
68% of the activity with 2-aminophenol
721454
Comamonas testosteroni
2-amino-5-chloromuconate 6-semialdehyde
-
-
-
?
2-aminophenol + O2
-
721454
Comamonas testosteroni
?
-
-
-
?
catechol + O2
5% of the activity with 2-aminophenol
721454
Comamonas testosteroni
?
-
-
-
?
additional information
no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol
721454
Comamonas testosteroni
?
-
-
-
?
protocatechuate + O2
33% of the activity with 2-aminophenol
721454
Comamonas testosteroni
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE
Comamonas testosteroni
Synonyms
Synonyms
Commentary
Organism
CnbcA
-
Comamonas testosteroni
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Comamonas testosteroni
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
2 mM, 79% residual activity
Comamonas testosteroni
Cd2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
Co2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
Cu2+
2 mM, 2.9% residual activity
Comamonas testosteroni
EDTA
2 mM, 50% residual activity
Comamonas testosteroni
Fe2+
2 mM, 57% residual activity. Not inhibitory in presence of ascorbate
Comamonas testosteroni
H2O2
2 mM, complete loss of activity
Comamonas testosteroni
Mg2+
2 mM, 91% residual activity
Comamonas testosteroni
Mn2+
2 mM, 31% residual activity
Comamonas testosteroni
Ni2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
Zn2+
2 mM abolishes enzyme activity completely
Comamonas testosteroni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00077
-
2-amino-5-chlorophenol
pH 8.0, 22°C
Comamonas testosteroni
0.00089
-
2-Aminophenol
pH 8.0, 22°C
Comamonas testosteroni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE
Comamonas testosteroni
38000
-
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE
Comamonas testosteroni
130000
-
gel filtration
Comamonas testosteroni
Purification (Commentary) (protein specific)
Commentary
Organism
-
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
29.7
-
pH 8.0, 22°C
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-amino-5-chlorophenol + O2
68% of the activity with 2-aminophenol
721454
Comamonas testosteroni
2-amino-5-chloromuconate 6-semialdehyde
-
-
-
?
2-aminophenol + O2
-
721454
Comamonas testosteroni
?
-
-
-
?
catechol + O2
5% of the activity with 2-aminophenol
721454
Comamonas testosteroni
?
-
-
-
?
additional information
no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol
721454
Comamonas testosteroni
?
-
-
-
?
protocatechuate + O2
33% of the activity with 2-aminophenol
721454
Comamonas testosteroni
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE
Comamonas testosteroni
Other publictions for EC 1.13.11.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
721454
Wu
A novel 2-aminophenol 1,6-diox ...
Comamonas testosteroni
Arch. Microbiol.
183
1-8
2005
-
-
1
-
-
-
11
2
-
-
3
-
-
1
-
-
1
-
-
-
1
-
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
11
-
2
-
-
3
-
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-