A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli

Wu, J.F.; Sun, C.W.; Jiang, C.Y.; Liu, Z.P.; Liu, S.J.; Arch. Microbiol. 183, 1-8 (2005)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Ca2+

2 mM, 79% residual activity

Comamonas testosteroni

Cd2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

Co2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

Cu2+

2 mM, 2.9% residual activity

Comamonas testosteroni

EDTA

2 mM, 50% residual activity

Comamonas testosteroni

Fe2+

2 mM, 57% residual activity. Not inhibitory in presence of ascorbate

Comamonas testosteroni

H2O2

2 mM, complete loss of activity

Comamonas testosteroni

Mg2+

2 mM, 91% residual activity

Comamonas testosteroni

Mn2+

2 mM, 31% residual activity

Comamonas testosteroni

Ni2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

Zn2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.00077

-

2-amino-5-chlorophenol

pH 8.0, 22°C

Comamonas testosteroni

0.00089

-

2-Aminophenol

pH 8.0, 22°C

Comamonas testosteroni

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2-amino-5-chlorophenol + O2

68% of the activity with 2-aminophenol

721454

Comamonas testosteroni

2-amino-5-chloromuconate 6-semialdehyde

-

-

-

?

2-aminophenol + O2

-

721454

Comamonas testosteroni

?

-

-

-

?

catechol + O2

5% of the activity with 2-aminophenol

721454

Comamonas testosteroni

?

-

-

-

?

additional information

no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol

721454

Comamonas testosteroni

?

-

-

-

?

protocatechuate + O2

33% of the activity with 2-aminophenol

721454

Comamonas testosteroni

?

-

-

-

?

Inhibitors

Commentary

Organism

Structure

Ca2+

2 mM, 79% residual activity

Comamonas testosteroni

Cd2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

Co2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

Cu2+

2 mM, 2.9% residual activity

Comamonas testosteroni

EDTA

2 mM, 50% residual activity

Comamonas testosteroni

Fe2+

2 mM, 57% residual activity. Not inhibitory in presence of ascorbate

Comamonas testosteroni

H2O2

2 mM, complete loss of activity

Comamonas testosteroni

Mg2+

2 mM, 91% residual activity

Comamonas testosteroni

Mn2+

2 mM, 31% residual activity

Comamonas testosteroni

Ni2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

Zn2+

2 mM abolishes enzyme activity completely

Comamonas testosteroni

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.00077

-

2-amino-5-chlorophenol

pH 8.0, 22°C

Comamonas testosteroni

0.00089

-

2-Aminophenol

pH 8.0, 22°C

Comamonas testosteroni

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2-amino-5-chlorophenol + O2

68% of the activity with 2-aminophenol

721454

Comamonas testosteroni

2-amino-5-chloromuconate 6-semialdehyde

-

-

-

?

2-aminophenol + O2

-

721454

Comamonas testosteroni

?

-

-

-

?

catechol + O2

5% of the activity with 2-aminophenol

721454

Comamonas testosteroni

?

-

-

-

?

additional information

no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol

721454

Comamonas testosteroni

?

-

-

-

?

protocatechuate + O2

33% of the activity with 2-aminophenol

721454

Comamonas testosteroni

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Protein Variants

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

kcat/KM [mM/s]

kcat/KM [mM/s] (protein specific)

721454

Wu

A novel 2-aminophenol 1,6-diox ...

Comamonas testosteroni

Arch. Microbiol.

183

1-8

2005

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