Literature summary for 1.13.11.79 extracted from

Yu, T.Y.; Mok, K.C.; Kennedy, K.J.; Valton, J.; Anderson, K.S.; Walker, G.C.; Taga, M.E.
Active site residues critical for flavin binding and 5,6-dimethylbenzimidazole biosynthesis in the flavin destructase enzyme BluB (2012), Protein Sci., 21, 839-849.

Search for more literature for 1.13.11.79

Cloned(Commentary)

Cloned (Comment)	Organism
gene bluB, genotyping	Sinorhizobium meliloti

Protein Variants

Protein Variants	Comment	Organism
A156V	site-directed mutagenesis, inactive mutant	Sinorhizobium meliloti
D32N	site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme	Sinorhizobium meliloti
D32N/S167G	site-directed mutagenesis, the mutant shows no activity	Sinorhizobium meliloti
E78K	site-directed mutagenesis, the mutant is not soluble	Sinorhizobium meliloti
G110S	site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity	Sinorhizobium meliloti
G133S	site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity	Sinorhizobium meliloti
G193D	site-directed mutagenesis, inactive mutant	Sinorhizobium meliloti
G61D	site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity	Sinorhizobium meliloti
M140I	site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme	Sinorhizobium meliloti
M94I	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Sinorhizobium meliloti
P202L	site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity	Sinorhizobium meliloti
P65L	site-directed mutagenesis, inactive mutant	Sinorhizobium meliloti
R30C	site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity	Sinorhizobium meliloti
R30H	site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity	Sinorhizobium meliloti
S167G	site-directed mutagenesis, the mutant shows higly reduced activity compared to the wild-type enzyme	Sinorhizobium meliloti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	FMN/FMNH2 dissociation constants and free energy of binding of wild-type and mutant enzymes, overview	Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
FMNH2 + NADH + H+ + O2	Sinorhizobium meliloti	-	5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?	5,6-dimethylbenzimidazole is the lower axial ligand of vitamin B12, i.e. cobalamin	?

Organism

Organism	UniProt	Comment	Textmining
Sinorhizobium meliloti	Q92PC8	gene bluB	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
FMNH2 + NADH + H+ + O2	-	Sinorhizobium meliloti	5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?	5,6-dimethylbenzimidazole is the lower axial ligand of vitamin B12, i.e. cobalamin	?
FMNH2 + NADH + H+ + O2	BluB fragments a flavin isoalloxazine ring	Sinorhizobium meliloti	5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?	-	?

Synonyms

Synonyms	Comment	Organism
BluB	-	Sinorhizobium meliloti
flavin destructase	-	Sinorhizobium meliloti
flavin destructase enzyme	-	Sinorhizobium meliloti

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Sinorhizobium meliloti

General Information

General Information	Comment	Organism
additional information	identification of catalytic residues involved in the reaction, mutational analysis, overview. The enzyme shows interactions with the phosphate group and ribityl tail of FMN	Sinorhizobium meliloti

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Release 2023.1 (January 2023)