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Literature summary for 1.13.11.8 extracted from
- Characterization of protocatechuate 4,5-dioxygenase induced from p-hydroxybenzoate-cultured Pseudomonas sp. K82 (2004), J. Microbiol., 42, 152-155.
General Stability
| General Stability | Organism |
|---|---|
| rapid loss of activity during purification due to the oxidation of an active-site ferrous iron group | Pseudomonas sp. K82 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Pseudomonas sp. K82 |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Pseudomonas sp. K82 | |
| Mn2+ | - |
Pseudomonas sp. K82 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
1 * 17000, + 1 * 34000, SDS-PAGE | Pseudomonas sp. K82 |
| 34000 | - |
1 * 17000, + 1 * 34000, SDS-PAGE | Pseudomonas sp. K82 |
| 54000 | - |
gel filtration | Pseudomonas sp. K82 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protocatechuate + O2 | Pseudomonas sp. K82 | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. K82 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Q-Sepharose | Pseudomonas sp. K82 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.14 | - |
- |
Pseudomonas sp. K82 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protocatechuate + O2 | - |
Pseudomonas sp. K82 | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 17000, + 1 * 34000, SDS-PAGE | Pseudomonas sp. K82 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCD 4,5 | - |
Pseudomonas sp. K82 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
- |
Pseudomonas sp. K82 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 35 | approx. 85% of maximal activity at 5°C, approx. 20% of maximal activity at 35°C | Pseudomonas sp. K82 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | 9 | - |
Pseudomonas sp. K82 |
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