Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.82 extracted from

  • Kim, Y.; Seo, E.; Oh, D.
    Characterization of an apo-carotenoid 13,14-dioxygenase from Novosphingobium aromaticivorans that converts beta-apo-8'-carotenal to beta-apo-13-carotenone (2012), Biotechnol. Lett., 34, 1851-1856 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Novosphingobium aromaticivorans

Protein Variants

Protein Variants Comment Organism
H170A the mutant has 0.08% iron content and 2.3% activity compared to the wild type enzyme Novosphingobium aromaticivorans
H219A the mutant has no iron content and 1.1% activity compared to the wild type enzyme Novosphingobium aromaticivorans
H284A the mutant has 0.14% iron content and 2% activity compared to the wild type enzyme Novosphingobium aromaticivorans
H460A the inactive mutant has no iron content Novosphingobium aromaticivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
8'-apo-beta-carotenal at pH 8.0 and 45┬░C Novosphingobium aromaticivorans
0.037
-
4'-apo-beta-carotenal at pH 8.0 and 45┬░C Novosphingobium aromaticivorans
0.203
-
gamma-carotene at pH 8.0 and 45┬░C Novosphingobium aromaticivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.01 mM Fe2+ strongly enhances the enzyme activity Novosphingobium aromaticivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
gel filtration Novosphingobium aromaticivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8'-apo-beta-carotenal + O2 Novosphingobium aromaticivorans
-
13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial
-
?
8'-apo-beta-carotenal + O2 Novosphingobium aromaticivorans DSM 12444
-
13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial
-
?

Organism

Organism UniProt Comment Textmining
Novosphingobium aromaticivorans
-
-
-
Novosphingobium aromaticivorans DSM 12444
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap affinity column chromatography Novosphingobium aromaticivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4'-apo-beta-carotenal + O2
-
Novosphingobium aromaticivorans ?
-
?
4'-apo-beta-carotenal + O2
-
Novosphingobium aromaticivorans DSM 12444 ?
-
?
8'-apo-beta-carotenal + O2
-
Novosphingobium aromaticivorans 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial
-
?
8'-apo-beta-carotenal + O2 best substrate Novosphingobium aromaticivorans 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial
-
?
8'-apo-beta-carotenal + O2
-
Novosphingobium aromaticivorans DSM 12444 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial
-
?
8'-apo-beta-carotenal + O2 best substrate Novosphingobium aromaticivorans DSM 12444 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial
-
?
gamma-carotene + O2
-
Novosphingobium aromaticivorans ?
-
?
gamma-carotene + O2
-
Novosphingobium aromaticivorans DSM 12444 ?
-
?
additional information no activity with alpha-carotene, beta-carotene, beta-cryptoxanthin, zeaxanthin, lutein, beta-apo-12'-carotenal, and lycopene Novosphingobium aromaticivorans ?
-
-
additional information no activity with alpha-carotene, beta-carotene, beta-cryptoxanthin, zeaxanthin, lutein, beta-apo-12'-carotenal, and lycopene Novosphingobium aromaticivorans DSM 12444 ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Novosphingobium aromaticivorans
monomer 1 * 52130, calculated from amino acid sequence Novosphingobium aromaticivorans

Synonyms

Synonyms Comment Organism
apo-carotenoid 13,14-dioxygenase
-
Novosphingobium aromaticivorans
NACOX1
-
Novosphingobium aromaticivorans

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
-
Novosphingobium aromaticivorans

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
35 50 60% or more activity between 35 and 50┬░C, about 25% activity at 55┬░C Novosphingobium aromaticivorans

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40 50 the half-lives of the enzyme at 40, 45, 50, 55 and 60┬░C are 23.4, 15.3, 8.2, 3.8, and 0.6 h, respectively Novosphingobium aromaticivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0033
-
gamma-carotene at pH 8.0 and 45┬░C Novosphingobium aromaticivorans
0.14
-
4'-apo-beta-carotenal at pH 8.0 and 45┬░C Novosphingobium aromaticivorans
0.417
-
8'-apo-beta-carotenal at pH 8.0 and 45┬░C Novosphingobium aromaticivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Novosphingobium aromaticivorans

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 60% activity between pH 7.0 and 9.0 Novosphingobium aromaticivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
gamma-carotene at pH 8.0 and 45┬░C Novosphingobium aromaticivorans
3.78
-
4'-apo-beta-carotenal at pH 8.0 and 45┬░C Novosphingobium aromaticivorans
19.57
-
8'-apo-beta-carotenal at pH 8.0 and 45┬░C Novosphingobium aromaticivorans