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Literature summary for 1.13.11.85 extracted from
- Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms (2014), Appl. Environ. Microbiol., 80, 5012-5020 .
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Xanthomonas sp. 35Y | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-1,4-polyisoprene + O2 | Xanthomonas sp. 35Y | - |
(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas sp. 35Y | Q7X0P3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Xanthomonas sp. 35Y |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-1,4-polyisoprene + O2 | - |
Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
| cis-1,4-polyisoprene + O2 | exo-type cleavage mechanism. RoxA is able to cleave isolated latex-clearing protein-derived oligo-isoprenoid molecules to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal groups | Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| roxA | - |
Xanthomonas sp. 35Y |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Xanthomonas sp. 35Y |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 30 | at 30°C, the specific activity of RoxA is almost double as high as at 23°C | Xanthomonas sp. 35Y |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
no significant reduction in activity after 22 h | Xanthomonas sp. 35Y |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | in potassium phosphate buffer or in HEPES buffer. Other buffers (Tris, carbonate-ammonium acetate, and glycylglycine) result in poor activities | Xanthomonas sp. 35Y |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH values below pH 6 or above 8.5 result in only poor activities | Xanthomonas sp. 35Y |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Xanthomonas sp. 35Y | |
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Release 2023.1 (January 2023)
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