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Literature summary for 1.13.11.89 extracted from

  • Gama, S.; Vogt, M.; Kalina, T.; Hupp, K.; Hammerschmidt, F.; Pallitsch, K.; Zechel, D.
    An oxidative pathway for microbial utilization of methylphosphonic acid as a phosphate source (2019), ACS Chem. Biol., 14, 735-741 .
    View publication on PubMed
Search for more literature for 1.13.11.89

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gimesia maris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 (hydroxymethyl)phosphonate pH 7.0, 25°C Gimesia maris
0.13
-
 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid pH 7.0, 25°C Gimesia maris
0.28
-
 (R)-1-hydroxyethylphosphonic acid pH 7.0, 25°C Gimesia maris

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Km value 0.010 mM for Fe2+ Gimesia maris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(hydroxymethyl)phosphonate + O2 Gimesia maris
-
 formate + phosphate
-
 ?
(hydroxymethyl)phosphonate + O2 Gimesia maris DSM 8797
-
 formate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gimesia maris
-
-
-
Gimesia maris DSM 8797
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R)-[2-amino-1-hydroxyethyl]phosphonic acid + O2 no substrate: (1S)-[2-amino-1-hydroxyethyl]phosphonic acid Gimesia maris aminoacetate + phosphate
-
 ?
(1R)-[2-amino-1-hydroxyethyl]phosphonic acid + O2 no substrate: (1S)-[2-amino-1-hydroxyethyl]phosphonic acid Gimesia maris DSM 8797 aminoacetate + phosphate
-
 ?
(hydroxymethyl)phosphonate + O2
-
 Gimesia maris formate + phosphate
-
 ?
(hydroxymethyl)phosphonate + O2
-
 Gimesia maris DSM 8797 formate + phosphate
-
 ?
(R)-1-hydroxyethylphosphonic acid + O2
-
 Gimesia maris acetate + phosphate
-
 ?
(R)-1-hydroxyethylphosphonic acid + O2
-
 Gimesia maris DSM 8797 acetate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
phnZ1
-
 Gimesia maris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gimesia maris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
 (hydroxymethyl)phosphonate pH 7.0, 25°C Gimesia maris
1.54
-
 (R)-1-hydroxyethylphosphonic acid pH 7.0, 25°C Gimesia maris
2.5
-
 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid pH 7.0, 25°C Gimesia maris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Gimesia maris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18
-
 (R)-1-hydroxyethylphosphonic acid pH 7.0, 25°C Gimesia maris
20
-
 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid pH 7.0, 25°C Gimesia maris
21
-
 (hydroxymethyl)phosphonate pH 7.0, 25°C Gimesia maris