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Literature summary for 1.13.11.9 extracted from

  • Cain, R.B.; Houghton, C.; Wright, K.A.
    Microbial metabolism of the pyridine ring. Metabolism of 2- and 3-hydroxypyridines by the maleamate pathway in Achromobacter sp (1974), Biochem. J., 140, 293-300.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.9

Activating Compound

Activating Compound Comment Organism Structure
L-cysteine enzyme has a specific requirement for L-cysteine (6.7 mM), required to restore full activity after dialysis or treatment with chelating agents Achromobacter sp.

General Stability

General Stability Organism
dialysis against phosphate buffer: complete loss of activity Achromobacter sp.
incubation with or dialysis against 0.001 mM 8-hydroxyquinoline and by (NH4)2SO4 fractionation: complete loss of activity Achromobacter sp.
L-cysteine: enzyme has a specific requirement for L-cysteine, 6.7 mM, required to restore full activity after dialysis or treatment with chelating agents Achromobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Pyridine-1,2-diol
-
 Achromobacter sp.
Pyridine-1,4-diol
-
 Achromobacter sp.
pyridine-2,3-diol
-
 Achromobacter sp.
pyridine-2,4-diol
-
 Achromobacter sp.
Pyridine-3,4-diol
-
 Achromobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Fe2+ requirement Achromobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
330000 340000 gel filtration Achromobacter sp.

Organism

Organism UniProt Comment Textmining
Achromobacter sp.
-
 G2 and 2L
-

Oxidation Stability

Oxidation Stability Organism
rapid loss of activity in air, purified enzyme, t1/2 is 2 h Achromobacter sp.

Purification (Commentary)

Purification (Comment) Organism
partial Achromobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.3
-
-
 Achromobacter sp.

Storage Stability

Storage Stability Organism
-20°C, stable for at least several weeks, enzyme in crude extract Achromobacter sp.
4°C, several days, enzyme in crude extract Achromobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxypyridine + O2 + H2O
-
 Achromobacter sp. maleamate + formate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Achromobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, enzyme in crude extract stable Achromobacter sp.
65
-
 10 min, enzyme in crude extract, 75% loss of activity Achromobacter sp.