Literature summary for 1.13.11.9 extracted from

Liu, G.; Zhao, Y.L.; He, F.; Zhang, P.; Ouyang, X.; Tang, H.; Xu, P.
Structure-guided insights into heterocyclic ring-cleavage catalysis of the non-heme Fe (II) dioxygenase NicX (2021), Nat. Commun., 12, 1301 .

Search for more literature for 1.13.11.9

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method at 20°C, crystal structure for the resting form of the enzyme (NicX), as well as a complex structure wherein 2,5-hydroxy-pyridine and N-formylmaleamic acid are trapped in different subunits. The resting state structure displays an octahedral coordination for Fe(II) with two histidine residues (His265 and His318), a serine residue (Ser302), a carboxylate ligand (Asp320), and two water molecules. 2,5-Hydroxy-pyridine does not bind as a ligand to Fe(II), yet its interactions with Leu104 and His105 function to guide and stabilize the substrate to the appropriate position to initiate the reaction	Pseudomonas putida

Crystallization (Comment)

Organism

hanging-drop vapor-diffusion method at 20°C, crystal structure for the resting form of the enzyme (NicX), as well as a complex structure wherein 2,5-hydroxy-pyridine and N-formylmaleamic acid are trapped in different subunits. The resting state structure displays an octahedral coordination for Fe(II) with two histidine residues (His265 and His318), a serine residue (Ser302), a carboxylate ligand (Asp320), and two water molecules. 2,5-Hydroxy-pyridine does not bind as a ligand to Fe(II), yet its interactions with Leu104 and His105 function to guide and stabilize the substrate to the appropriate position to initiate the reaction

Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme uses a mononuclear non-heme iron enzyme to catalyze the oxidative pyridine ring cleavage reaction	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,5-dihydroxypyridine + O2	Pseudomonas putida	-	N-formylmaleamic acid	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	Q88FY1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,5-dihydroxypyridine + O2	-	Pseudomonas putida	N-formylmaleamic acid	-	?

Synonyms

Synonyms	Comment	Organism
2,5-dihydroxypyridine dioxygenase	-	Pseudomonas putida
NicX	-	Pseudomonas putida

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Release 2023.1 (January 2023)