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Literature summary for 1.13.11.91 extracted from

  • Aloi, S.; Davies, C.G.; Karplus, P.A.; Wilbanks, S.M.; Jameson, G.N.L.
    Substrate specificity in thiol dioxygenases (2019), Biochemistry, 58, 2398-2407 .
    View publication on PubMed
Search for more literature for 1.13.11.91

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas aeruginosa
-
 Cupriavidus necator

Protein Variants

Protein Variants Comment Organism
Q62R variant retains both 3-mercaptopropanoate and cysteine dioxygenase activity Pseudomonas aeruginosa
R168A variant does not fold properly Pseudomonas aeruginosa
R168C variant does not fold properly Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 (2R)-2-sulfanylbutanedioic acid mutant Q62R, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
0.7
-
 3-Mercaptopropanoate pH not specified in the publication, temperature not specified in the publication Cupriavidus necator
0.8
-
 3-Mercaptopropanoate wild-type, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
0.9
-
 3-Mercaptopropanoate mutant Q62R, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q9I0N5 cf. EC 1.13.11.91
-
Cupriavidus necator JMP 134 Q9I0N5 cf. EC 1.13.11.91
-
Pseudomonas aeruginosa Q9I0N5 cf. EC 1.13.11.91
-
Pseudomonas aeruginosa DSM 22644 Q9I0N5 cf. EC 1.13.11.91
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-2-sulfanylbutanedioic acid + O2 i.e. (R)-mercaptosuccinate, no substrate for wild-type, but mutant Q62R Pseudomonas aeruginosa ?
-
 ?
(2R)-2-sulfanylbutanedioic acid + O2 i.e. (R)-mercaptosuccinate, no substrate for wild-type, but mutant Q62R Pseudomonas aeruginosa DSM 22644 ?
-
 ?
3-mercaptopropanoate + O2
-
 Pseudomonas aeruginosa 3-sulfinopropanoate
-
 ?
3-mercaptopropanoate + O2
-
 Cupriavidus necator 3-sulfinopropanoate
-
 ?
3-mercaptopropanoate + O2
-
 Pseudomonas aeruginosa DSM 22644 3-sulfinopropanoate
-
 ?
3-mercaptopropanoate + O2
-
 Cupriavidus necator JMP 134 3-sulfinopropanoate
-
 ?
L-cysteine + O2 plus some cysteine sulfinic acid, reaction of EC 1.13.11.20 Pseudomonas aeruginosa 3-sulfinoalanine
-
 ?
L-cysteine + O2 plus some cysteine sulfinic acid, reaction of EC 1.13.11.20 Pseudomonas aeruginosa DSM 22644 3-sulfinoalanine
-
 ?
additional information enzyme shows only minor cysteine dioxygenase activity Cupriavidus necator ?
-
-
additional information enzyme shows only minor cysteine dioxygenase activity Cupriavidus necator JMP 134 ?
-
-

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 (2R)-2-sulfanylbutanedioic acid mutant Q62R, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
0.28
-
 3-Mercaptopropanoate wild-type, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
0.35
-
 3-Mercaptopropanoate pH not specified in the publication, temperature not specified in the publication Cupriavidus necator
0.67
-
 3-Mercaptopropanoate mutant Q62R, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 (2R)-2-sulfanylbutanedioic acid mutant Q62R, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
0.35
-
 3-Mercaptopropanoate wild-type, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
0.5
-
 3-Mercaptopropanoate pH not specified in the publication, temperature not specified in the publication Cupriavidus necator
0.74
-
 3-Mercaptopropanoate mutant Q62R, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa