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Literature summary for 1.13.11.91 extracted from

  • Tchesnokov, E.P.; Fellner, M.; Siakkou, E.; Kleffmann, T.; Martin, L.W.; Aloi, S.; Lamont, I.L.; Wilbanks, S.M.; Jameson, G.N.
    The cysteine dioxygenase homologue from Pseudomonas aeruginosa is a 3-mercaptopropionate dioxygenase (2015), J. Biol. Chem., 290, 24424-24437 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.91

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
comparison with mammalian cysteine dioxygenase. The overall active site geometry is conserved but the different substrate specificity may be related to replacement of an arginine by a glutamine in the active site Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 3-Mercaptopropanoate pH 7.5, 37°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron active site iron is coordinated by 3 histidines and 3 water molecules Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-mercaptopropanoate + O2 Pseudomonas aeruginosa
-
 3-sulfinopropanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I0N5
-
-
Pseudomonas aeruginosa DSM 22644 Q9I0N5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification no posttranslational modification observed Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopropanoate + O2
-
 Pseudomonas aeruginosa 3-sulfinopropanoate
-
 ?
L-cysteine + O2 reaction of EC 1.13.11.20 Pseudomonas aeruginosa 3-sulfinoalanine
-
 ?
L-cysteine + O2 reaction of EC 1.13.11.20 Pseudomonas aeruginosa DSM 22644 3-sulfinoalanine
-
 ?
additional information recombinant 3MDO is able to oxidize both cysteine and 3-mercaptopropionic acid in vitro, with a marked preference for 3-mercaptopropionic acid. Substrate binding to the ferrous iron is through the thiol but each substrate may adopt different coordination geometries Pseudomonas aeruginosa ?
-
-
additional information recombinant 3MDO is able to oxidize both cysteine and 3-mercaptopropionic acid in vitro, with a marked preference for 3-mercaptopropionic acid. Substrate binding to the ferrous iron is through the thiol but each substrate may adopt different coordination geometries Pseudomonas aeruginosa DSM 22644 ?
-
-

Subunits

Subunits Comment Organism
? x * 23746, calculated from sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
3MDO
-
 Pseudomonas aeruginosa
PA2602
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 3-Mercaptopropanoate pH 7.5, 37°C Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa low expression under standard growth conditions additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
110
-
 3-Mercaptopropanoate pH 7.5, 37°C Pseudomonas aeruginosa