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Literature summary for 1.13.11.92 extracted from

  • Liu, W.; Rogge, C.E.; Bambai, B.; Palmer, G.; Tsai, A.L.; Kulmacz, R.J.
    Characterization of the heme environment in Arabidopsis thaliana fatty acid alpha-dioxygenase-1 (2004), J. Biol. Chem., 279, 29805-29815 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and insect cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
H163C mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
H163E mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
H163M mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
H163Y mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
H389C mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
H389E mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
H389M mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
H389Y mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
cyanide
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
oleic acid pH 7.8, 30°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana A0A178V8W6 isoform PADOX-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
pH 7.8, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid + O2
-
Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z,12Z)-octadeca-9,12-dienoic acid
-
?
linolenic acid + O2
-
Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid
-
?
myristic acid + O2
-
Arabidopsis thaliana (2R)-2-hydroperoxy-tetradecanoic acid
-
?
oleic acid + O2
-
Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z)-octadeca-9-enoic acid
-
?

Subunits

Subunits Comment Organism
? x * 98000, SDS-PAGE, GST-tagged recombinant protein Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AXX17_At3g00510
-
Arabidopsis thaliana
PADOX-1
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
heme 1 mol of heme bound tightly but noncovalently per protein monomer. The high spin ferric heme can be reduced to the ferrous state by dithionite Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
cyanide pH 7.8, 30°C Arabidopsis thaliana