Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and insect cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
H163C | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
H163E | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
H163M | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
H163Y | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
H389C | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
H389E | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
H389M | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
H389Y | mutation results in perturbation of the heme absorbance spectrum and oxygenase activity | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
oleic acid | pH 7.8, 30°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | A0A178V8W6 | isoform PADOX-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
pH 7.8, 30°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + O2 | - |
Arabidopsis thaliana | (2R)-2-hydroperoxy-(9Z,12Z)-octadeca-9,12-dienoic acid | - |
? | |
linolenic acid + O2 | - |
Arabidopsis thaliana | (2R)-2-hydroperoxy-(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid | - |
? | |
myristic acid + O2 | - |
Arabidopsis thaliana | (2R)-2-hydroperoxy-tetradecanoic acid | - |
? | |
oleic acid + O2 | - |
Arabidopsis thaliana | (2R)-2-hydroperoxy-(9Z)-octadeca-9-enoic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, SDS-PAGE, GST-tagged recombinant protein | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AXX17_At3g00510 | - |
Arabidopsis thaliana |
PADOX-1 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | 1 mol of heme bound tightly but noncovalently per protein monomer. The high spin ferric heme can be reduced to the ferrous state by dithionite | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
cyanide | pH 7.8, 30°C | Arabidopsis thaliana |