Literature summary for 1.13.11.92 extracted from

Liu, W.; Wang, L.; Fabian, P.; Hayashi, Y.; McGinley, C.; van der Donk, W.; Kulmacz, R.
Arabidopsisxa0thaliana fatty acid alpha-dioxygenase-1 evaluation ofxa0substrates, inhibitors andxa0amino-terminal function (2006), Plant Physiol. Biochem., 44, 284-293 .

Search for more literature for 1.13.11.92

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
3,4-dehydromyristate	inactivates the oxygenase without appreciable oxygen consumption	Arabidopsis thaliana
3-thiamyristate	reaction leads to self-inactivation of enzyme	Arabidopsis thaliana
catechol	-	Arabidopsis thaliana
Co2+	-	Arabidopsis thaliana
cyanide	-	Arabidopsis thaliana
N,N,N',N'-tetramethyl-4-phenylenediamine	-	Arabidopsis thaliana
Zn2+	presence of Zn2+ does not change the Km values for linoleate or oxygen, or the Ki value for cyanide	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SGH6	isoform DOX1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Arabidopsis thaliana

Storage Stability

Storage Stability	Organism
4°C, 6 months, 90% residual activity	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-thiamyristate + O2	-	Arabidopsis thaliana	(2R)-2-hydroperoxy-3-thiatetradecanoic acid	-	?
gamma-linolenic acid + O2	-	Arabidopsis thaliana	?	-	?
linoleic acid + O2	-	Arabidopsis thaliana	(2R)-2-hydroperoxy-(9Z,12Z)-octadeca-9,12-dienoic acid	-	?
linolenic acid + O2	-	Arabidopsis thaliana	(2R)-2-hydroperoxy-(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid	-	?
additional information	no substrates: methyl esters of myristate, linoleate and linolenate are not oxygenated	Arabidopsis thaliana	?	-	-
myristic acid + O2	-	Arabidopsis thaliana	(2R)-2-hydroperoxy-tetradecanoic acid	-	?
oleic acid + O2	-	Arabidopsis thaliana	(2R)-2-hydroperoxy-(9Z)-octadeca-9-enoic acid	-	?
palmitic acid + O2	-	Arabidopsis thaliana	(R,S)-2-hydroperoxypalmitic acid	-	?
palmitoleic acid + O2	-	Arabidopsis thaliana	(R,S)-2-hydroperoxypalmitic acid	-	?

Subunits

Subunits	Comment	Organism
?	x * 98000, SDS-PAGE, recombinant GST-fusion protein	Arabidopsis thaliana
More	PADOX1 cleaved from GST-PADOX1 fusion by thrombin forms large multimeric complexes of 6-10 monomers. Full length PADOX1 expressed via a pCW vector in bacteria is a tetramer. Full length PADOX1 expressed in Sf9 cells chromatographed as a monomer	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.005	-	cyanide	30°C, pH 7.8	Arabidopsis thaliana
0.01	-	Co2+	30°C, pH 7.8	Arabidopsis thaliana
0.02	-	N,N,N',N'-tetramethyl-4-phenylenediamine	30°C, pH 7.8	Arabidopsis thaliana
0.05	-	Zn2+	30°C, pH 7.8	Arabidopsis thaliana
0.09	-	catechol	30°C, pH 7.8	Arabidopsis thaliana

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Release 2023.1 (January 2023)