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Literature summary for 1.13.11.93 extracted from

  • Thompson, M.; Blake-Hedges, J.; Cruz-Morales, P.; Barajas, J.; Curran, S.; Eiben, C.; Harris, N.; Benites, V.; Gin, J.; Sharpless, W.; Twigg, F.; Skyrud, W.; Krishna, R.; Pereira, J.; Baidoo, E.; Petzold, C.; Adams, P.; Arkin, A.; Deutschbauer, A.; Keasling, J.D.
    Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism (2019), mBio, 10, e02577-18 .
    View publication on PubMedView publication on EuropePMC
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Inhibitors

Inhibitors Comment Organism Structure
EDTA complete loss of activity, only presence of Fe(II) restores activity Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 2-oxoadipate pH 7, 30°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoadipate + O2 Pseudomonas putida
-
 (R)-2-hydroxyglutarate + CO2
-
 ?
2-oxoadipate + O2 Pseudomonas putida DSM 6125
-
 (R)-2-hydroxyglutarate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88CC1
-
-
Pseudomonas putida DSM 6125 Q88CC1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoadipate + O2
-
 Pseudomonas putida (R)-2-hydroxyglutarate + CO2
-
 ?
2-oxoadipate + O2
-
 Pseudomonas putida DSM 6125 (R)-2-hydroxyglutarate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
PP_5260
-
 Pseudomonas putida
ydcJ
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
 2-oxoadipate pH 7, 30°C Pseudomonas putida

General Information

General Information Comment Organism
evolution member of the DUF1338 protein family Pseudomonas putida
physiological function enzyme is involved in an L-lysine catabolic pathway. A gene deletion strain is unable to grow on either isomer of lysine Pseudomonas putida