Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme and enzyme from pea seed | Pisum sativum | |
6.7 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme and enzyme from pea seed | Pisum sativum | |
6.9 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme and enzyme from pea seed | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
x * 94000, SDS-PAGE | Pisum sativum |
97000 | - |
x * 97000, SDS-PAGE | Pisum sativum |
97134 | - |
x * 97134, calculated from sequence | Pisum sativum |
97628 | - |
x * 97628, calculated from sequence | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | P09918 | L. cv. Bite | - |
Pisum sativum | P14856 | L. cv. Bite | - |
Purification (Comment) | Organism |
---|---|
- |
Pisum sativum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 linoleate + 2 O2 = (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate | reaction mechanism is different from LOX-3 | Pisum sativum | |
2 linoleate + 2 O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | the reaction mechanism is different from LOX-2 | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
enzyme from pea seed | Pisum sativum |
18 | - |
recombinant enzyme | Pisum sativum |
40 | - |
recombinant enzyme | Pisum sativum |
48 | - |
enzyme from pea seed | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | - |
Pisum sativum | (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate | the ratio of (9Z,11E)-13-hydroperoxy-11,13-octadecadienoate to (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 1:2 for pea seed LOX-3 and recombinant LOX-3. The R/S stereoconfiguration of the products is not determined | ? | |
linoleate + O2 | - |
Pisum sativum | (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate | the ratio of (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate to (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 4:1 for LOX-2 from from pea seed and 7:1 for the recombinant enzyme LOX-2. The R/S stereoconfiguration of the product is not determined | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, SDS-PAGE | Pisum sativum |
? | x * 94000, SDS-PAGE | Pisum sativum |
? | x * 97134, calculated from sequence | Pisum sativum |
? | x * 97628, calculated from sequence | Pisum sativum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme and enzyme from pea seed | Pisum sativum | |
65 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme | Pisum sativum | |
78 | - |
linoleate | pH 6.3, 25°C, enzyme from pea seed | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | 6.5 | enzyme from pea seed | Pisum sativum |
5.7 | 6.7 | recombinant enzyme | Pisum sativum |
5.8 | 6.4 | recombinant enzyme and enzyme from pea seed | Pisum sativum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme and enzyme from pea seed | Pisum sativum | |
9.4 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme and enzyme from pea seed | Pisum sativum | |
12.2 | - |
linoleate | pH 6.3, 25°C, recombinant enzyme and enzyme from pea seed | Pisum sativum |