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Literature summary for 1.13.11.B6 extracted from

  • Hughes, R.K.; Wu, Z.; Robinson, D.S.; Hardy, D.; West, S.I.; Fairhurst, S.A.; Casey, R.
    Characterization of authentic recombinant pea-seed lipoxygenases with distinct properties and reaction mechanisms (1998), Biochem. J., 333, 33-43.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
linoleate pH 6.3, 25┬░C, recombinant enzyme and enzyme from pea seed Pisum sativum
6.7
-
linoleate pH 6.3, 25┬░C, recombinant enzyme and enzyme from pea seed Pisum sativum
6.9
-
linoleate pH 6.3, 25┬░C, recombinant enzyme and enzyme from pea seed Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
x * 94000, SDS-PAGE Pisum sativum
97000
-
x * 97000, SDS-PAGE Pisum sativum
97134
-
x * 97134, calculated from sequence Pisum sativum
97628
-
x * 97628, calculated from sequence Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum P09918 L. cv. Bite
-
Pisum sativum P14856 L. cv. Bite
-

Purification (Commentary)

Purification (Comment) Organism
-
Pisum sativum

Reaction

Reaction Comment Organism Reaction ID
2 linoleate + 2 O2 = (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate reaction mechanism is different from LOX-3 Pisum sativum
2 linoleate + 2 O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate the reaction mechanism is different from LOX-2 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
1.2
-
enzyme from pea seed Pisum sativum
18
-
recombinant enzyme Pisum sativum
40
-
recombinant enzyme Pisum sativum
48
-
enzyme from pea seed Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
-
Pisum sativum (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate the ratio of (9Z,11E)-13-hydroperoxy-11,13-octadecadienoate to (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 1:2 for pea seed LOX-3 and recombinant LOX-3. The R/S stereoconfiguration of the products is not determined ?
linoleate + O2
-
Pisum sativum (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate the ratio of (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate to (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 4:1 for LOX-2 from from pea seed and 7:1 for the recombinant enzyme LOX-2. The R/S stereoconfiguration of the product is not determined ?

Subunits

Subunits Comment Organism
? x * 97000, SDS-PAGE Pisum sativum
? x * 94000, SDS-PAGE Pisum sativum
? x * 97134, calculated from sequence Pisum sativum
? x * 97628, calculated from sequence Pisum sativum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
linoleate pH 6.3, 25┬░C, recombinant enzyme and enzyme from pea seed Pisum sativum
65
-
linoleate pH 6.3, 25┬░C, recombinant enzyme Pisum sativum
78
-
linoleate pH 6.3, 25┬░C, enzyme from pea seed Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6.5 enzyme from pea seed Pisum sativum
5.7 6.7 recombinant enzyme Pisum sativum
5.8 6.4 recombinant enzyme and enzyme from pea seed Pisum sativum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.7
-
linoleate pH 6.3, 25┬░C, recombinant enzyme and enzyme from pea seed Pisum sativum
9.4
-
linoleate pH 6.3, 25┬░C, recombinant enzyme and enzyme from pea seed Pisum sativum
12.2
-
linoleate pH 6.3, 25┬░C, recombinant enzyme and enzyme from pea seed Pisum sativum