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Literature summary for 1.13.11.B6 extracted from

  • Hughes, R.K.; West, S.I.; Hornostaj, A.R.; Lawson, D.M.; Fairhurst, S.A.; Sanchez, R.O.; Hough, P.; Robinson, B.H.; Casey, R.
    Probing a novel potato lipoxygenase with dual positional specificity reveals primary determinants of substrate binding and requirements for a surface hydrophobic loop and has implications for the role of lipoxygenases in tubers (2001), Biochem. J., 353, 345-355.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia col BL21(DE3) Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
I437L KM-value for linoleate is 114% of wild-type value, kcat/Km for linoleate is 83% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.7 (compared to 0.8 in reaction with wild-type enzyme) Solanum tuberosum
V570I KM-value for linoleate is 61% of wild-type value, kcat/Km for linoleate is 367% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.6 (compared to 0.8 in reaction with wild-type enzyme) Solanum tuberosum
V580F KM-value for linoleate is 384% of wild-type value, kcat/Km for linoleate is 4% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.9 (compared to 0.8 in reaction with wild-type enzyme) Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 linoleate pH 5.5, 25°C Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum Q9SC16 cv. Desiree
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber potato 13/9-LOX is only a minor component of the total LOX population in tubers Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65
-
-
 Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
-
 Solanum tuberosum (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate dual positional specificity: the enzyme produces roughly equimolar amounts of (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate. The R/S stereoconfiguration of the products is not determined ?
additional information the enzyme catalyzes oxidation of arachidonate with lower activity Solanum tuberosum ?
-
 ?

Synonyms

Synonyms Comment Organism
13/9 lipoxygenase
-
 Solanum tuberosum
13/9-LOX
-
 Solanum tuberosum
lox1-St-2
-
 Solanum tuberosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
106
-
 linoleate pH 5.5, 25°C Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 45% of maximal activity, pH 7.0: about 55% of maximal activity Solanum tuberosum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
530
-
 linoleate pH 5.5, 25°C Solanum tuberosum