BRENDA - Enzyme Database
show all sequences of 1.13.12.13

Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system

Inouye, S.; Sahara, Y.; Biochem. Biophys. Res. Commun. 376, 448-453 (2008) View publication on PubMed

Data extracted from this reference:

Application
Application
Commentary
Organism
molecular biology
useful reporter protein in various assay systems including reporter assays and immunoassays
Oplophorus gracilirostris
Cloned(Commentary)
Cloned (Commentary)
Organism
construction of a cold induced expression vector in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of protein A (ZZ-domain) and the multiple cloning sites, the role of ZZ-domain as a solubilizing partner at 15°C is demonstrated by expressing the imidazopyrazinone-type luciferase of Oplophorus
Oplophorus gracilirostris
Organism
Organism
UniProt
Commentary
Textmining
Oplophorus gracilirostris
Q9GV45
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
0.1% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), precipitate
Oplophorus gracilirostris
additional information
-
100% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), supernatant
Oplophorus gracilirostris
additional information
-
2.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), supernatant
Oplophorus gracilirostris
additional information
-
4.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), precipitate
Oplophorus gracilirostris
Synonyms
Synonyms
Commentary
Organism
imidazopyrazinone-type luciferase
-
Oplophorus gracilirostris
Oplophorus luciferase
-
Oplophorus gracilirostris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Oplophorus gracilirostris
Application (protein specific)
Application
Commentary
Organism
molecular biology
useful reporter protein in various assay systems including reporter assays and immunoassays
Oplophorus gracilirostris
Cloned(Commentary) (protein specific)
Commentary
Organism
construction of a cold induced expression vector in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of protein A (ZZ-domain) and the multiple cloning sites, the role of ZZ-domain as a solubilizing partner at 15°C is demonstrated by expressing the imidazopyrazinone-type luciferase of Oplophorus
Oplophorus gracilirostris
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
0.1% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), precipitate
Oplophorus gracilirostris
additional information
-
100% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), supernatant
Oplophorus gracilirostris
additional information
-
2.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), supernatant
Oplophorus gracilirostris
additional information
-
4.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), precipitate
Oplophorus gracilirostris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Oplophorus gracilirostris
Other publictions for EC 1.13.12.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
744231
Tomabechi
Crystal structure of nanoKAZ ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
470
88-93
2016
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744220
Inouye
Luminescence enhancement of t ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
445
157-162
2014
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744223
Inouye
Unconventional secretion of t ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
450
1313-1319
2014
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726882
Inouye
C6-Deoxy coelenterazine analog ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
437
23-28
2013
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728741
Inouye
Expression, purification and l ...
Oplophorus gracilirostris
Protein Expr. Purif.
88
150-156
2013
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727290
Hall
Engineered luciferase reporter ...
Oplophorus gracilirostris
Chem. Biol.
7
1848-1857
2012
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696008
Inouye
Soluble protein expression in ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
376
448-453
2008
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689868
Inouye
Overexpression, purification a ...
Oplophorus gracilirostris
Protein Expr. Purif.
56
261-268
2007
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674080
Kakoi
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Chemical studies of fish biolu ...
Diaphus gigas
ITE Lett. Batteries New Technol. Med.
6
38-45
2005
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657385
Nakamura
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Efficient bioluminescence of b ...
Oplophorus gracilirostris
Tetrahedron Lett.
38
6405-6406
1997
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654469
Yamaguchi
Oplophorus oxyluciferin and a ...
Oplophorus gracilirostris
Biochem. J.
151
9-15
1975
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