Reference to 1.13.12.13; Id = 696008 - BRENDA Enzyme Database

molecular biology

useful reporter protein in various assay systems including reporter assays and immunoassays

Oplophorus gracilirostris

construction of a cold induced expression vector in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of protein A (ZZ-domain) and the multiple cloning sites, the role of ZZ-domain as a solubilizing partner at 15Â°C is demonstrated by expressing the imidazopyrazinone-type luciferase of Oplophorus

Oplophorus gracilirostris

Q9GV45

-

additional information

-

0.1% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), precipitate

Oplophorus gracilirostris

additional information

-

100% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), supernatant

Oplophorus gracilirostris

additional information

-

2.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), supernatant

Oplophorus gracilirostris

additional information

-

4.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), precipitate

Oplophorus gracilirostris

imidazopyrazinone-type luciferase

-

Oplophorus gracilirostris

Oplophorus luciferase

-

Oplophorus gracilirostris

7.6

-

assay at

Oplophorus gracilirostris

molecular biology

useful reporter protein in various assay systems including reporter assays and immunoassays

Oplophorus gracilirostris

construction of a cold induced expression vector in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of protein A (ZZ-domain) and the multiple cloning sites, the role of ZZ-domain as a solubilizing partner at 15Â°C is demonstrated by expressing the imidazopyrazinone-type luciferase of Oplophorus

Oplophorus gracilirostris

additional information

-

0.1% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), precipitate

Oplophorus gracilirostris

additional information

-

100% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), supernatant

Oplophorus gracilirostris

additional information

-

2.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), supernatant

Oplophorus gracilirostris

additional information

-

4.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), precipitate

Oplophorus gracilirostris

7.6

-

assay at

Oplophorus gracilirostris

744231

Tomabechi

Crystal structure of nanoKAZ ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

470

88-93

2016

-

1

-

1

-

1

2

-

3

-

1

-

1

-

3

2

-

1

-

1

-

1

-

1

2

-

1

-

3

2

-

2

-

744220

Inouye

Luminescence enhancement of t ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

445

157-162

2014

-

1

-

21

-

1

-

1

3

-

5

-

1

-

9

1

2

-

1

-

1

-

21

-

1

-

1

3

-

1

-

9

1

-

1

-

1

-

744223

Inouye

Unconventional secretion of t ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

450

1313-1319

2014

-

1

-

1

-

1

-

1

2

-

2

-

2

1

2

-

1

-

1

-

1

-

1

2

-

2

1

-

1

-

726882

Inouye

C6-Deoxy coelenterazine analog ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

437

23-28

2013

-

1

-

1

-

6

-

1

-

7

-

2

-

1

-

1

-

1

-

1

-

7

-

1

-

728741

Inouye

Expression, purification and l ...

Oplophorus gracilirostris

Protein Expr. Purif.

88

150-156

2013

-

1

-

1

-

1

-

11

-

2

-

1

-

1

-

1

-

11

-

1

-

727290

Hall

Engineered luciferase reporter ...

Oplophorus gracilirostris

Chem. Biol.

7

1848-1857

2012

-

1

-

3

-

5

-

2

-

2

-

2

-

1

-

3

-

2

-

2

-

696008

Inouye

Soluble protein expression in ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

376

448-453

2008

-

1

-

1

-

4

-

2

-

1

-

1

-

4

-

1

-

689868

Inouye

Overexpression, purification a ...

Oplophorus gracilirostris

Protein Expr. Purif.

56

261-268

2007

-

1

-

3

-

3

4

1

-

1

-

1

-

5

1

-

1

-

3

-

3

-

4

1

-

1

-

5

1

-

674080

Kakoi

-

Chemical studies of fish biolu ...

Diaphus gigas

ITE Lett. Batteries New Technol. Med.

6

38-45

2005

-

1

-

1

-

1

-

1

-

1

-

657385

Nakamura

-

Efficient bioluminescence of b ...

Oplophorus gracilirostris

Tetrahedron Lett.

38

6405-6406

1997

-

1

-

2

-

2

-

654469

Yamaguchi

Oplophorus oxyluciferin and a ...

Oplophorus gracilirostris

Biochem. J.

151

9-15

1975

-

1

-

1

-

1

-

1

-

1

-

Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system

Data extracted from this reference: