Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines

Inouye, S.; Sahara-Miura, Y.; Sato, J.; Iimori, R.; Yoshida, S.; Hosoya, T.; Protein Expr. Purif. 88, 150-156 (2013)

Data extracted from this reference:

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

3iso-coelenterazine + O2

luminescence intesity (Imax): 78.2%

728741

Oplophorus gracilirostris

oxidized 3iso-coelenterazine + CO2 + hn

-

-

-

?

3me-coelenterazine + O2

luminescence intesity (Imax): 80%

728741

Oplophorus gracilirostris

oxidized 3me-coelenterazine + CO2 + hn

-

-

-

?

3meo-coelenterazine + O2

luminescence intesity (Imax): 189%

728741

Oplophorus gracilirostris

oxidized 3meo-coelenterazine + CO2 + hn

-

-

-

?

alphameh-coelenterazine + O2

luminescence intesity (Imax): 15.7%

728741

Oplophorus gracilirostris

oxidized alphameh-coelenterazine + CO2 + hn

-

-

-

?

cf3-coelenterazine + O2

luminescence intesity (Imax): 49.5%

728741

Oplophorus gracilirostris

oxidized cf3-coelenterazine + CO2 + hn

-

-

-

?

coelenterazine + O2

luminescence intesity (Imax): 100%

728741

Oplophorus gracilirostris

oxidized coelenterazine + CO2 + hn

-

-

-

?

et-coelenterazine + O2

luminescence intesity (Imax): 21.5%

728741

Oplophorus gracilirostris

oxidized et-coelenterazine + CO2 + hn

-

-

-

?

h-coelenterazine + O2

luminescence intesity (Imax): 68.4%

728741

Oplophorus gracilirostris

oxidized h-coelenterazine + CO2 + hn

-

-

-

?

i-coelenterazine + O2

luminescence intesity (Imax): 32.3%

728741

Oplophorus gracilirostris

oxidized i-coelenterazine + CO2 + hn

-

-

-

?

me-coelenterazine + O2

luminescence intesity (Imax): 46.6%

728741

Oplophorus gracilirostris

oxidized me-coelenterazine + CO2 + hn

-

-

-

?

meo-coelenterazine + O2

luminescence intesity (Imax): 68.1%

728741

Oplophorus gracilirostris

oxidized meo-coelenterazine + CO2 + hn

-

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

3iso-coelenterazine + O2

luminescence intesity (Imax): 78.2%

728741

Oplophorus gracilirostris

oxidized 3iso-coelenterazine + CO2 + hn

-

-

-

?

3me-coelenterazine + O2

luminescence intesity (Imax): 80%

728741

Oplophorus gracilirostris

oxidized 3me-coelenterazine + CO2 + hn

-

-

-

?

3meo-coelenterazine + O2

luminescence intesity (Imax): 189%

728741

Oplophorus gracilirostris

oxidized 3meo-coelenterazine + CO2 + hn

-

-

-

?

alphameh-coelenterazine + O2

luminescence intesity (Imax): 15.7%

728741

Oplophorus gracilirostris

oxidized alphameh-coelenterazine + CO2 + hn

-

-

-

?

cf3-coelenterazine + O2

luminescence intesity (Imax): 49.5%

728741

Oplophorus gracilirostris

oxidized cf3-coelenterazine + CO2 + hn

-

-

-

?

coelenterazine + O2

luminescence intesity (Imax): 100%

728741

Oplophorus gracilirostris

oxidized coelenterazine + CO2 + hn

-

-

-

?

et-coelenterazine + O2

luminescence intesity (Imax): 21.5%

728741

Oplophorus gracilirostris

oxidized et-coelenterazine + CO2 + hn

-

-

-

?

h-coelenterazine + O2

luminescence intesity (Imax): 68.4%

728741

Oplophorus gracilirostris

oxidized h-coelenterazine + CO2 + hn

-

-

-

?

i-coelenterazine + O2

luminescence intesity (Imax): 32.3%

728741

Oplophorus gracilirostris

oxidized i-coelenterazine + CO2 + hn

-

-

-

?

me-coelenterazine + O2

luminescence intesity (Imax): 46.6%

728741

Oplophorus gracilirostris

oxidized me-coelenterazine + CO2 + hn

-

-

-

?

meo-coelenterazine + O2

luminescence intesity (Imax): 68.1%

728741

Oplophorus gracilirostris

oxidized meo-coelenterazine + CO2 + hn

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

744231

Tomabechi

Crystal structure of nanoKAZ ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

470

88-93

2016

-

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1

1

1

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2

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744220

Inouye

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Oplophorus gracilirostris

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1

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21

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21

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9

1

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1

1

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744223

Inouye

Unconventional secretion of t ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

450

1313-1319

2014

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1

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1

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1

1

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726882

Inouye

C6-Deoxy coelenterazine analog ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

437

23-28

2013

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1

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6

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7

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7

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728741

Inouye

Expression, purification and l ...

Oplophorus gracilirostris

Protein Expr. Purif.

88

150-156

2013

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1

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1

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11

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2

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1

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11

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1

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727290

Hall

Engineered luciferase reporter ...

Oplophorus gracilirostris

Chem. Biol.

7

1848-1857

2012

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1

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696008

Inouye

Soluble protein expression in ...

Oplophorus gracilirostris

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376

448-453

2008

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1

1

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Inouye

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Oplophorus gracilirostris

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