BRENDA - Enzyme Database
show all sequences of 1.13.12.13

Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines

Inouye, S.; Sahara-Miura, Y.; Sato, J.; Iimori, R.; Yoshida, S.; Hosoya, T.; Protein Expr. Purif. 88, 150-156 (2013) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
the catalytic domain of Oplophorus luciferase (19kOLase) is expressed in Escherichia coli cells using the cold-induced vectors of pCold-KAZ. Cold induction for Escherichia coli cells is carried out at 15°C
Oplophorus gracilirostris
Organism
Organism
UniProt
Commentary
Textmining
Oplophorus gracilirostris
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
using nickel-chelate affinity column
Oplophorus gracilirostris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3iso-coelenterazine + O2
luminescence intesity (Imax): 78.2%
728741
Oplophorus gracilirostris
oxidized 3iso-coelenterazine + CO2 + hn
-
-
-
?
3me-coelenterazine + O2
luminescence intesity (Imax): 80%
728741
Oplophorus gracilirostris
oxidized 3me-coelenterazine + CO2 + hn
-
-
-
?
3meo-coelenterazine + O2
luminescence intesity (Imax): 189%
728741
Oplophorus gracilirostris
oxidized 3meo-coelenterazine + CO2 + hn
-
-
-
?
alphameh-coelenterazine + O2
luminescence intesity (Imax): 15.7%
728741
Oplophorus gracilirostris
oxidized alphameh-coelenterazine + CO2 + hn
-
-
-
?
cf3-coelenterazine + O2
luminescence intesity (Imax): 49.5%
728741
Oplophorus gracilirostris
oxidized cf3-coelenterazine + CO2 + hn
-
-
-
?
coelenterazine + O2
luminescence intesity (Imax): 100%
728741
Oplophorus gracilirostris
oxidized coelenterazine + CO2 + hn
-
-
-
?
et-coelenterazine + O2
luminescence intesity (Imax): 21.5%
728741
Oplophorus gracilirostris
oxidized et-coelenterazine + CO2 + hn
-
-
-
?
h-coelenterazine + O2
luminescence intesity (Imax): 68.4%
728741
Oplophorus gracilirostris
oxidized h-coelenterazine + CO2 + hn
-
-
-
?
i-coelenterazine + O2
luminescence intesity (Imax): 32.3%
728741
Oplophorus gracilirostris
oxidized i-coelenterazine + CO2 + hn
-
-
-
?
me-coelenterazine + O2
luminescence intesity (Imax): 46.6%
728741
Oplophorus gracilirostris
oxidized me-coelenterazine + CO2 + hn
-
-
-
?
meo-coelenterazine + O2
luminescence intesity (Imax): 68.1%
728741
Oplophorus gracilirostris
oxidized meo-coelenterazine + CO2 + hn
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
19kOLase
-
Oplophorus gracilirostris
luciferase
-
Oplophorus gracilirostris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Oplophorus gracilirostris
Cloned(Commentary) (protein specific)
Commentary
Organism
the catalytic domain of Oplophorus luciferase (19kOLase) is expressed in Escherichia coli cells using the cold-induced vectors of pCold-KAZ. Cold induction for Escherichia coli cells is carried out at 15°C
Oplophorus gracilirostris
Purification (Commentary) (protein specific)
Commentary
Organism
using nickel-chelate affinity column
Oplophorus gracilirostris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3iso-coelenterazine + O2
luminescence intesity (Imax): 78.2%
728741
Oplophorus gracilirostris
oxidized 3iso-coelenterazine + CO2 + hn
-
-
-
?
3me-coelenterazine + O2
luminescence intesity (Imax): 80%
728741
Oplophorus gracilirostris
oxidized 3me-coelenterazine + CO2 + hn
-
-
-
?
3meo-coelenterazine + O2
luminescence intesity (Imax): 189%
728741
Oplophorus gracilirostris
oxidized 3meo-coelenterazine + CO2 + hn
-
-
-
?
alphameh-coelenterazine + O2
luminescence intesity (Imax): 15.7%
728741
Oplophorus gracilirostris
oxidized alphameh-coelenterazine + CO2 + hn
-
-
-
?
cf3-coelenterazine + O2
luminescence intesity (Imax): 49.5%
728741
Oplophorus gracilirostris
oxidized cf3-coelenterazine + CO2 + hn
-
-
-
?
coelenterazine + O2
luminescence intesity (Imax): 100%
728741
Oplophorus gracilirostris
oxidized coelenterazine + CO2 + hn
-
-
-
?
et-coelenterazine + O2
luminescence intesity (Imax): 21.5%
728741
Oplophorus gracilirostris
oxidized et-coelenterazine + CO2 + hn
-
-
-
?
h-coelenterazine + O2
luminescence intesity (Imax): 68.4%
728741
Oplophorus gracilirostris
oxidized h-coelenterazine + CO2 + hn
-
-
-
?
i-coelenterazine + O2
luminescence intesity (Imax): 32.3%
728741
Oplophorus gracilirostris
oxidized i-coelenterazine + CO2 + hn
-
-
-
?
me-coelenterazine + O2
luminescence intesity (Imax): 46.6%
728741
Oplophorus gracilirostris
oxidized me-coelenterazine + CO2 + hn
-
-
-
?
meo-coelenterazine + O2
luminescence intesity (Imax): 68.1%
728741
Oplophorus gracilirostris
oxidized meo-coelenterazine + CO2 + hn
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Oplophorus gracilirostris
Other publictions for EC 1.13.12.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744231
Tomabechi
Crystal structure of nanoKAZ ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
470
88-93
2016
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744220
Inouye
Luminescence enhancement of t ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
445
157-162
2014
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21
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9
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21
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744223
Inouye
Unconventional secretion of t ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
450
1313-1319
2014
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726882
Inouye
C6-Deoxy coelenterazine analog ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
437
23-28
2013
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1
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6
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728741
Inouye
Expression, purification and l ...
Oplophorus gracilirostris
Protein Expr. Purif.
88
150-156
2013
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1
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1
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11
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11
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727290
Hall
Engineered luciferase reporter ...
Oplophorus gracilirostris
Chem. Biol.
7
1848-1857
2012
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696008
Inouye
Soluble protein expression in ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
376
448-453
2008
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1
1
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4
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689868
Inouye
Overexpression, purification a ...
Oplophorus gracilirostris
Protein Expr. Purif.
56
261-268
2007
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3
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4
1
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674080
Kakoi
-
Chemical studies of fish biolu ...
Diaphus gigas
ITE Lett. Batteries New Technol. Med.
6
38-45
2005
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657385
Nakamura
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Efficient bioluminescence of b ...
Oplophorus gracilirostris
Tetrahedron Lett.
38
6405-6406
1997
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654469
Yamaguchi
Oplophorus oxyluciferin and a ...
Oplophorus gracilirostris
Biochem. J.
151
9-15
1975
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