Reference to 1.13.12.13; Id = 744223 - BRENDA Enzyme Database

recombinant expression and secretion of 19 kDa catalytic subunit mutant nanoKAZ from CHO-K1 cells. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway. Recombinant expression of His-tagged wild-type and mutant KAZs in Escherichia coli. The secreted proteins form aggregates

Oplophorus gracilirostris

additional information

construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway, Construction of other DELTA1-15 truncated mutants of the catalytic subunit KAZ, i.e. DN2T, DN3L, DN4E, DN5D, DN6F, DN7V, DN8G, DN9D, DN10W, DN15G, and DN20Q. The mutant enzyme activity is dependent on the mutation and the type of expression vector, overview. Secretory expression of nanoKAZ from mammalian cells in the absence of the N-terminal signal peptide

Oplophorus gracilirostris

extracellular

the enzyme possesses an amino-terminal signal peptide and is secreted

Oplophorus gracilirostris

-

106000

-

full-length enzyme

Oplophorus gracilirostris

coelenterazine + O2

Oplophorus gracilirostris

-

coelenteramide + CO2 + hv

-

?

Oplophorus luciferin + O2

Oplophorus gracilirostris

-

oxidized Oplophorus luciferin + CO2 + hv

-

?

Oplophorus gracilirostris

Q9GV45 AND Q9GV46

catalytic and noncatalytic subunit

-

coelenterazine + O2

-

744223

Oplophorus gracilirostris

coelenteramide + CO2 + hv

-

?

Oplophorus luciferin + O2

-

744223

Oplophorus gracilirostris

oxidized Oplophorus luciferin + CO2 + hv

-

?

heterooligomer

x * 19000, catalytic subunit, + x * 35000, noncatalytic subunit, SDS-PAGE

Oplophorus gracilirostris

KAZ

catalytic enzyme subunit

Oplophorus gracilirostris

Oplophorus luciferase

-

Oplophorus gracilirostris

recombinant expression and secretion of 19 kDa catalytic subunit mutant nanoKAZ from CHO-K1 cells. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway. Recombinant expression of His-tagged wild-type and mutant KAZs in Escherichia coli. The secreted proteins form aggregates

Oplophorus gracilirostris

additional information

construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway, Construction of other DELTA1-15 truncated mutants of the catalytic subunit KAZ, i.e. DN2T, DN3L, DN4E, DN5D, DN6F, DN7V, DN8G, DN9D, DN10W, DN15G, and DN20Q. The mutant enzyme activity is dependent on the mutation and the type of expression vector, overview. Secretory expression of nanoKAZ from mammalian cells in the absence of the N-terminal signal peptide

Oplophorus gracilirostris

extracellular

the enzyme possesses an amino-terminal signal peptide and is secreted

Oplophorus gracilirostris

-

106000

-

full-length enzyme

Oplophorus gracilirostris

coelenterazine + O2

Oplophorus gracilirostris

-

coelenteramide + CO2 + hv

-

?

Oplophorus luciferin + O2

Oplophorus gracilirostris

-

oxidized Oplophorus luciferin + CO2 + hv

-

?

coelenterazine + O2

-

744223

Oplophorus gracilirostris

coelenteramide + CO2 + hv

-

?

Oplophorus luciferin + O2

-

744223

Oplophorus gracilirostris

oxidized Oplophorus luciferin + CO2 + hv

-

?

heterooligomer

x * 19000, catalytic subunit, + x * 35000, noncatalytic subunit, SDS-PAGE

Oplophorus gracilirostris

malfunction

recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway

Oplophorus gracilirostris

malfunction

recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway

Oplophorus gracilirostris

744231

Tomabechi

Crystal structure of nanoKAZ ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

470

88-93

2016

-

1

-

1

-

1

2

-

3

-

1

-

1

-

3

2

-

1

-

1

-

1

-

1

2

-

1

-

3

2

-

2

-

744220

Inouye

Luminescence enhancement of t ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

445

157-162

2014

-

1

-

21

-

1

-

1

3

-

5

-

1

-

9

1

2

-

1

-

1

-

21

-

1

-

1

3

-

1

-

9

1

-

1

-

1

-

744223

Inouye

Unconventional secretion of t ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

450

1313-1319

2014

-

1

-

1

-

1

-

1

2

-

2

-

2

1

2

-

1

-

1

-

1

-

1

2

-

2

1

-

1

-

726882

Inouye

C6-Deoxy coelenterazine analog ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

437

23-28

2013

-

1

-

1

-

6

-

1

-

7

-

2

-

1

-

1

-

1

-

1

-

7

-

1

-

728741

Inouye

Expression, purification and l ...

Oplophorus gracilirostris

Protein Expr. Purif.

88

150-156

2013

-

1

-

1

-

1

-

11

-

2

-

1

-

1

-

1

-

11

-

1

-

727290

Hall

Engineered luciferase reporter ...

Oplophorus gracilirostris

Chem. Biol.

7

1848-1857

2012

-

1

-

3

-

5

-

2

-

2

-

2

-

1

-

3

-

2

-

2

-

696008

Inouye

Soluble protein expression in ...

Oplophorus gracilirostris

Biochem. Biophys. Res. Commun.

376

448-453

2008

-

1

-

1

-

4

-

2

-

1

-

1

-

4

-

1

-

689868

Inouye

Overexpression, purification a ...

Oplophorus gracilirostris

Protein Expr. Purif.

56

261-268

2007

-

1

-

3

-

3

4

1

-

1

-

1

-

5

1

-

1

-

3

-

3

-

4

1

-

1

-

5

1

-

674080

Kakoi

-

Chemical studies of fish biolu ...

Diaphus gigas

ITE Lett. Batteries New Technol. Med.

6

38-45

2005

-

1

-

1

-

1

-

1

-

1

-

657385

Nakamura

-

Efficient bioluminescence of b ...

Oplophorus gracilirostris

Tetrahedron Lett.

38

6405-6406

1997

-

1

-

2

-

2

-

654469

Yamaguchi

Oplophorus oxyluciferin and a ...

Oplophorus gracilirostris

Biochem. J.

151

9-15

1975

-

1

-

1

-

1

-

1

-

1

-

Unconventional secretion of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells

Data extracted from this reference: