BRENDA - Enzyme Database
show all sequences of 1.13.12.13

Unconventional secretion of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells

Inouye, S.; Sato, J.; Sahara-Miura, Y.; Hosoya, T.; Suzuki, T.; Biochem. Biophys. Res. Commun. 450, 1313-1319 (2014) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
recombinant expression and secretion of 19 kDa catalytic subunit mutant nanoKAZ from CHO-K1 cells. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway. Recombinant expression of His-tagged wild-type and mutant KAZs in Escherichia coli. The secreted proteins form aggregates
Oplophorus gracilirostris
Engineering
Protein Variants
Commentary
Organism
additional information
construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway, Construction of other DELTA1-15 truncated mutants of the catalytic subunit KAZ, i.e. DN2T, DN3L, DN4E, DN5D, DN6F, DN7V, DN8G, DN9D, DN10W, DN15G, and DN20Q. The mutant enzyme activity is dependent on the mutation and the type of expression vector, overview. Secretory expression of nanoKAZ from mammalian cells in the absence of the N-terminal signal peptide
Oplophorus gracilirostris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
the enzyme possesses an amino-terminal signal peptide and is secreted
Oplophorus gracilirostris
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
106000
-
full-length enzyme
Oplophorus gracilirostris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coelenterazine + O2
Oplophorus gracilirostris
-
coelenteramide + CO2 + hv
-
-
?
Oplophorus luciferin + O2
Oplophorus gracilirostris
-
oxidized Oplophorus luciferin + CO2 + hv
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Oplophorus gracilirostris
Q9GV45 AND Q9GV46
catalytic and noncatalytic subunit
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
coelenterazine + O2
-
744223
Oplophorus gracilirostris
coelenteramide + CO2 + hv
-
-
-
?
Oplophorus luciferin + O2
-
744223
Oplophorus gracilirostris
oxidized Oplophorus luciferin + CO2 + hv
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterooligomer
x * 19000, catalytic subunit, + x * 35000, noncatalytic subunit, SDS-PAGE
Oplophorus gracilirostris
Synonyms
Synonyms
Commentary
Organism
KAZ
catalytic enzyme subunit
Oplophorus gracilirostris
Oplophorus luciferase
-
Oplophorus gracilirostris
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression and secretion of 19 kDa catalytic subunit mutant nanoKAZ from CHO-K1 cells. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway. Recombinant expression of His-tagged wild-type and mutant KAZs in Escherichia coli. The secreted proteins form aggregates
Oplophorus gracilirostris
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway, Construction of other DELTA1-15 truncated mutants of the catalytic subunit KAZ, i.e. DN2T, DN3L, DN4E, DN5D, DN6F, DN7V, DN8G, DN9D, DN10W, DN15G, and DN20Q. The mutant enzyme activity is dependent on the mutation and the type of expression vector, overview. Secretory expression of nanoKAZ from mammalian cells in the absence of the N-terminal signal peptide
Oplophorus gracilirostris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
the enzyme possesses an amino-terminal signal peptide and is secreted
Oplophorus gracilirostris
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
106000
-
full-length enzyme
Oplophorus gracilirostris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coelenterazine + O2
Oplophorus gracilirostris
-
coelenteramide + CO2 + hv
-
-
?
Oplophorus luciferin + O2
Oplophorus gracilirostris
-
oxidized Oplophorus luciferin + CO2 + hv
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
coelenterazine + O2
-
744223
Oplophorus gracilirostris
coelenteramide + CO2 + hv
-
-
-
?
Oplophorus luciferin + O2
-
744223
Oplophorus gracilirostris
oxidized Oplophorus luciferin + CO2 + hv
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterooligomer
x * 19000, catalytic subunit, + x * 35000, noncatalytic subunit, SDS-PAGE
Oplophorus gracilirostris
General Information
General Information
Commentary
Organism
malfunction
recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway
Oplophorus gracilirostris
General Information (protein specific)
General Information
Commentary
Organism
malfunction
recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway
Oplophorus gracilirostris
Other publictions for EC 1.13.12.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744231
Tomabechi
Crystal structure of nanoKAZ ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
470
88-93
2016
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1
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2
2
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744220
Inouye
Luminescence enhancement of t ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
445
157-162
2014
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1
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21
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1
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5
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9
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21
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1
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744223
Inouye
Unconventional secretion of t ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
450
1313-1319
2014
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1
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1
1
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726882
Inouye
C6-Deoxy coelenterazine analog ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
437
23-28
2013
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1
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6
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728741
Inouye
Expression, purification and l ...
Oplophorus gracilirostris
Protein Expr. Purif.
88
150-156
2013
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1
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11
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11
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727290
Hall
Engineered luciferase reporter ...
Oplophorus gracilirostris
Chem. Biol.
7
1848-1857
2012
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1
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2
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696008
Inouye
Soluble protein expression in ...
Oplophorus gracilirostris
Biochem. Biophys. Res. Commun.
376
448-453
2008
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1
1
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1
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4
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4
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1
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689868
Inouye
Overexpression, purification a ...
Oplophorus gracilirostris
Protein Expr. Purif.
56
261-268
2007
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1
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3
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4
1
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674080
Kakoi
-
Chemical studies of fish biolu ...
Diaphus gigas
ITE Lett. Batteries New Technol. Med.
6
38-45
2005
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657385
Nakamura
-
Efficient bioluminescence of b ...
Oplophorus gracilirostris
Tetrahedron Lett.
38
6405-6406
1997
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654469
Yamaguchi
Oplophorus oxyluciferin and a ...
Oplophorus gracilirostris
Biochem. J.
151
9-15
1975
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