BRENDA - Enzyme Database
show all sequences of 1.13.12.16

Purification, characterization, and mechanism of a flavin mononucleotide-dependent 2-nitropropane dioxygenase from Neurospora crassa

Gorlatova, N.; Tchorzewski, M.; Kurihara, T.; Soda, K.; Esaki, N.; Appl. Environ. Microbiol. 64, 1029-1033 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
1 mM, relative activity remaining 2%
Neurospora crassa
cytochrome c
0.04 mM, relative activity remaining 34%
Neurospora crassa
dithiothreitol
1 mM, complete inhibition
Neurospora crassa
EDTA
1 mM, relative activity remaining 95%
Neurospora crassa
GSH
5 mM, relative activity remaining 28%
Neurospora crassa
iodoacetate
1 mM, complete inhibition
Neurospora crassa
Nitro blue tetrazolium
2.5 mM, relative activity remaining 10%
Neurospora crassa
Superoxide dismutase
complete inhibition
Neurospora crassa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
3
nitroethane
neutral form of substrate
Neurospora crassa
3.1
-
2-Nitropropane
anionic form of substrate
Neurospora crassa
3.1
-
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
6
-
2-Nitropropane
anionic form of substrate
Neurospora crassa
6
-
ethyl nitronate
anionic form of substrate
Neurospora crassa
8.3
-
1-Nitropropane
anionic form of substrate
Neurospora crassa
8.3
-
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
20
-
1-Nitropropane
neutral form of substrate
Neurospora crassa
23
-
nitroethane
neutral form of substrate
Neurospora crassa
33
-
2-Nitropropane
neutral form of substrate
Neurospora crassa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Neurospora crassa
70000
-
gel filtration
Neurospora crassa
Organism
Organism
UniProt
Commentary
Textmining
Neurospora crassa
-
-
-
Neurospora crassa
-
ATCC 10337
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Neurospora crassa
to homogeneity
Neurospora crassa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10000
-
-
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-hydroxybutyl-2-nitronate + O2
-
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
-
-
-
?
1-hydroxyethyl-2-nitronate + O2
-
639232
Neurospora crassa
glycoaldehyde + HNO2
-
-
-
?
1-nitropentane + O2
3% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
-
-
-
?
1-nitropropane + O2
21% of the activity with 2-nitropropane
639232
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
2-hydroxypentyl-3-nitronate + O2
-
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
-
-
-
?
2-nitroethanol + O2
13% of the activity with 2-nitropropane
639232
Neurospora crassa
glycoaldehyde + HNO2
-
-
-
?
2-nitropropane + O2
-
639232
Neurospora crassa
acetone + HNO2
-
-
-
?
3-nitro-2-butanol + O2
14% of the activity with 2-nitropropane
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
-
-
-
?
3-nitro-2-pentanol + O2
20% of the activity with 2-nitropropane
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
-
-
-
?
3-nitropropionic acid + O2
12% of the activity with 2-nitropropane
639232
Neurospora crassa
?
-
-
-
?
cyclohexyl nitronate + O2
-
639232
Neurospora crassa
? + HNO2
-
-
-
?
ethyl nitronate + O2
-
639232
Neurospora crassa
acetaldehyde + HNO2
-
-
-
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds
639232
Neurospora crassa
?
-
-
-
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates
639232
Neurospora crassa
?
-
-
-
?
nitrocyclohexane + O2
2% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
-
-
-
?
nitroethane + O2
27% of the activity with 2-nitropropane
639232
Neurospora crassa
acetaldehyde + HNO2
-
-
-
?
pentyl-1-nitronate + O2
-
639232
Neurospora crassa
? + HNO2
-
-
-
?
propionate-3-nitronate + O2
-
639232
Neurospora crassa
?
-
-
-
?
propyl-1-nitronate + O2
-
639232
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
propyl-2-nitronate + O2
-
639232
Neurospora crassa
acetone + HNO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Neurospora crassa
Synonyms
Synonyms
Commentary
Organism
2-nitropropane dioxygenase
-
Neurospora crassa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Neurospora crassa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
32
-
nitroethane
neutral form of substrate
Neurospora crassa
35
-
1-Nitropropane
neutral form of substrate
Neurospora crassa
160
-
2-Nitropropane
neutral form of substrate
Neurospora crassa
1300
-
1-Nitropropane
anionic form of substrate
Neurospora crassa
1300
-
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
2000
-
nitroethane
anionic form of substrate
Neurospora crassa
2000
-
ethyl nitronate
anionic form of substrate
Neurospora crassa
6400
-
2-Nitropropane
anionic form of substrate
Neurospora crassa
6400
-
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Neurospora crassa
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
2 mol of FMN per mol of subunit
Neurospora crassa
FMN
dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit
Neurospora crassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
2 mol of FMN per mol of subunit
Neurospora crassa
FMN
dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit
Neurospora crassa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Neurospora crassa
2-mercaptoethanol
1 mM, relative activity remaining 2%
Neurospora crassa
cytochrome c
-
Neurospora crassa
cytochrome c
0.04 mM, relative activity remaining 34%
Neurospora crassa
dithiothreitol
-
Neurospora crassa
dithiothreitol
1 mM, complete inhibition
Neurospora crassa
EDTA
1 mM, relative activity remaining 95%
Neurospora crassa
GSH
-
Neurospora crassa
GSH
5 mM, relative activity remaining 28%
Neurospora crassa
iodoacetate
-
Neurospora crassa
iodoacetate
1 mM, complete inhibition
Neurospora crassa
Nitro blue tetrazolium
-
Neurospora crassa
Nitro blue tetrazolium
2.5 mM, relative activity remaining 10%
Neurospora crassa
Superoxide dismutase
-
Neurospora crassa
Superoxide dismutase
complete inhibition
Neurospora crassa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
3
nitroethane
neutral form of substrate
Neurospora crassa
3.1
-
2-Nitropropane
anionic form of substrate
Neurospora crassa
3.1
-
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
6
-
2-Nitropropane
anionic form of substrate
Neurospora crassa
6
-
ethyl nitronate
anionic form of substrate
Neurospora crassa
8.3
-
1-Nitropropane
anionic form of substrate
Neurospora crassa
8.3
-
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
20
-
1-Nitropropane
neutral form of substrate
Neurospora crassa
23
-
nitroethane
neutral form of substrate
Neurospora crassa
33
-
2-Nitropropane
neutral form of substrate
Neurospora crassa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Neurospora crassa
70000
-
gel filtration
Neurospora crassa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Neurospora crassa
to homogeneity
Neurospora crassa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10000
-
-
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-hydroxybutyl-2-nitronate + O2
-
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
-
-
-
?
1-hydroxyethyl-2-nitronate + O2
-
639232
Neurospora crassa
glycoaldehyde + HNO2
-
-
-
?
1-nitropentane + O2
3% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
-
-
-
?
1-nitropropane + O2
21% of the activity with 2-nitropropane
639232
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
2-hydroxypentyl-3-nitronate + O2
-
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
-
-
-
?
2-nitroethanol + O2
13% of the activity with 2-nitropropane
639232
Neurospora crassa
glycoaldehyde + HNO2
-
-
-
?
2-nitropropane + O2
-
639232
Neurospora crassa
acetone + HNO2
-
-
-
?
3-nitro-2-butanol + O2
14% of the activity with 2-nitropropane
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
-
-
-
?
3-nitro-2-pentanol + O2
20% of the activity with 2-nitropropane
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
-
-
-
?
3-nitropropionic acid + O2
12% of the activity with 2-nitropropane
639232
Neurospora crassa
?
-
-
-
?
cyclohexyl nitronate + O2
-
639232
Neurospora crassa
? + HNO2
-
-
-
?
ethyl nitronate + O2
-
639232
Neurospora crassa
acetaldehyde + HNO2
-
-
-
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds
639232
Neurospora crassa
?
-
-
-
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates
639232
Neurospora crassa
?
-
-
-
?
nitrocyclohexane + O2
2% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
-
-
-
?
nitroethane + O2
27% of the activity with 2-nitropropane
639232
Neurospora crassa
acetaldehyde + HNO2
-
-
-
?
pentyl-1-nitronate + O2
-
639232
Neurospora crassa
? + HNO2
-
-
-
?
propionate-3-nitronate + O2
-
639232
Neurospora crassa
?
-
-
-
?
propyl-1-nitronate + O2
-
639232
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
propyl-2-nitronate + O2
-
639232
Neurospora crassa
acetone + HNO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Neurospora crassa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Neurospora crassa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
32
-
nitroethane
neutral form of substrate
Neurospora crassa
35
-
1-Nitropropane
neutral form of substrate
Neurospora crassa
160
-
2-Nitropropane
neutral form of substrate
Neurospora crassa
1300
-
1-Nitropropane
anionic form of substrate
Neurospora crassa
1300
-
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
2000
-
nitroethane
anionic form of substrate
Neurospora crassa
2000
-
ethyl nitronate
anionic form of substrate
Neurospora crassa
6400
-
2-Nitropropane
anionic form of substrate
Neurospora crassa
6400
-
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Neurospora crassa
Other publictions for EC 1.13.12.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745219
Vercammen
Pseudomonas aeruginosa LysR P ...
Pseudomonas aeruginosa
J. Bacteriol.
197
1026-1039
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-
1
-
1
-
-
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-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745306
Salvi
The combined structural and k ...
Pseudomonas aeruginosa
J. Biol. Chem.
289
23764-23775
2014
-
-
1
1
-
-
-
4
-
-
-
1
-
10
-
-
1
1
-
-
-
-
8
1
5
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
8
1
1
-
-
3
1
-
-
-
-
3
3
-
6
6
727018
Smitherman
Evidence for a transient perox ...
Cyberlindnera saturnus
Biochemistry
52
2694-2704
2013
-
-
-
-
-
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
1
2
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
5
1
-
-
-
-
-
-
-
6
6
728077
Klinkenberg
Rv1894c is a novel hypoxia-ind ...
Mycobacterium tuberculosis
J. Infect. Dis.
207
1525-1534
2013
-
-
1
-
1
-
1
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
726808
Francis
A novel activity for fungal ni ...
Cyberlindnera saturnus, Neurospora crassa
Arch. Biochem. Biophys.
521
84-89
2012
-
-
-
-
-
-
-
3
-
-
-
-
-
10
-
-
-
-
-
-
-
-
3
-
4
2
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
3
2
-
-
-
-
3
3
-
3
3
724175
Li
Crystal structure and site-dir ...
Streptomyces ansochromogenes
Biochem. Biophys. Res. Commun.
405
344-348
2011
-
-
1
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii
Arch. Biochem. Biophys.
493
53-61
2009
-
-
-
-
-
-
-
1
-
3
-
13
-
6
-
-
-
3
-
-
-
-
16
1
3
3
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
3
-
13
-
-
-
-
-
-
-
-
16
1
3
-
-
-
3
-
-
-
-
-
-
-
-
-
696315
Francis
Inflated kinetic isotope effec ...
Neurospora crassa
Biochemistry
48
2403-2410
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684712
Mijatovic
Oxidation of alkyl nitronates ...
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Kido
Purification and properties of ...
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