Data extracted from this reference:
Inhibitors
2-mercaptoethanol
1 mM, relative activity remaining 2%
Neurospora crassa
cytochrome c
0.04 mM, relative activity remaining 34%
Neurospora crassa
dithiothreitol
1 mM, complete inhibition
Neurospora crassa
EDTA
1 mM, relative activity remaining 95%
Neurospora crassa
GSH
5 mM, relative activity remaining 28%
Neurospora crassa
iodoacetate
1 mM, complete inhibition
Neurospora crassa
Nitro blue tetrazolium
2.5 mM, relative activity remaining 10%
Neurospora crassa
Superoxide dismutase
complete inhibition
Neurospora crassa
KM Value [mM]
2
3
nitroethane
neutral form of substrate
Neurospora crassa
3.1
2-Nitropropane
anionic form of substrate
Neurospora crassa
3.1
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
6
2-Nitropropane
anionic form of substrate
Neurospora crassa
6
ethyl nitronate
anionic form of substrate
Neurospora crassa
8.3
1-Nitropropane
anionic form of substrate
Neurospora crassa
8.3
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
20
1-Nitropropane
neutral form of substrate
Neurospora crassa
23
nitroethane
neutral form of substrate
Neurospora crassa
33
2-Nitropropane
neutral form of substrate
Neurospora crassa
Molecular Weight [Da]
40000
2 * 40000, SDS-PAGE
Neurospora crassa
70000
gel filtration
Neurospora crassa
Organism
Neurospora crassa
ATCC 10337
Purification (Commentary)
to homogeneity
Neurospora crassa
Source Tissue
mycelium
Neurospora crassa
Specific Activity [micromol/min/mg]
Substrates and Products (Substrate)
1-hydroxybutyl-2-nitronate + O2
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
?
1-hydroxyethyl-2-nitronate + O2
639232
Neurospora crassa
glycoaldehyde + HNO2
?
1-nitropentane + O2
3% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
?
1-nitropropane + O2
21% of the activity with 2-nitropropane
639232
Neurospora crassa
propionaldehyde + HNO2
?
2-hydroxypentyl-3-nitronate + O2
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
?
2-nitroethanol + O2
13% of the activity with 2-nitropropane
639232
Neurospora crassa
glycoaldehyde + HNO2
?
2-nitropropane + O2
639232
Neurospora crassa
acetone + HNO2
?
3-nitro-2-butanol + O2
14% of the activity with 2-nitropropane
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
?
3-nitro-2-pentanol + O2
20% of the activity with 2-nitropropane
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
?
3-nitropropionic acid + O2
12% of the activity with 2-nitropropane
639232
Neurospora crassa
?
?
cyclohexyl nitronate + O2
639232
Neurospora crassa
? + HNO2
?
ethyl nitronate + O2
639232
Neurospora crassa
acetaldehyde + HNO2
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds
639232
Neurospora crassa
?
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates
639232
Neurospora crassa
?
?
nitrocyclohexane + O2
2% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
?
nitroethane + O2
27% of the activity with 2-nitropropane
639232
Neurospora crassa
acetaldehyde + HNO2
?
pentyl-1-nitronate + O2
639232
Neurospora crassa
? + HNO2
?
propionate-3-nitronate + O2
639232
Neurospora crassa
?
?
propyl-1-nitronate + O2
639232
Neurospora crassa
propionaldehyde + HNO2
?
propyl-2-nitronate + O2
639232
Neurospora crassa
acetone + HNO2
?
Subunits
dimer
2 * 40000, SDS-PAGE
Neurospora crassa
Synonyms
2-nitropropane dioxygenase
Neurospora crassa
Temperature Optimum [°C]
30
assay at
Neurospora crassa
Turnover Number [1/s]
32
nitroethane
neutral form of substrate
Neurospora crassa
35
1-Nitropropane
neutral form of substrate
Neurospora crassa
160
2-Nitropropane
neutral form of substrate
Neurospora crassa
1300
1-Nitropropane
anionic form of substrate
Neurospora crassa
1300
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
2000
nitroethane
anionic form of substrate
Neurospora crassa
2000
ethyl nitronate
anionic form of substrate
Neurospora crassa
6400
2-Nitropropane
anionic form of substrate
Neurospora crassa
6400
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
pH Optimum
6.5
assay at
Neurospora crassa
Cofactor
FMN
2 mol of FMN per mol of subunit
Neurospora crassa
FMN
dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit
Neurospora crassa
Cofactor (protein specific)
FMN
2 mol of FMN per mol of subunit
Neurospora crassa
FMN
dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit
Neurospora crassa
Inhibitors (protein specific)
2-mercaptoethanol
Neurospora crassa
2-mercaptoethanol
1 mM, relative activity remaining 2%
Neurospora crassa
cytochrome c
Neurospora crassa
cytochrome c
0.04 mM, relative activity remaining 34%
Neurospora crassa
dithiothreitol
Neurospora crassa
dithiothreitol
1 mM, complete inhibition
Neurospora crassa
EDTA
1 mM, relative activity remaining 95%
Neurospora crassa
GSH
5 mM, relative activity remaining 28%
Neurospora crassa
iodoacetate
Neurospora crassa
iodoacetate
1 mM, complete inhibition
Neurospora crassa
Nitro blue tetrazolium
Neurospora crassa
Nitro blue tetrazolium
2.5 mM, relative activity remaining 10%
Neurospora crassa
Superoxide dismutase
Neurospora crassa
Superoxide dismutase
complete inhibition
Neurospora crassa
KM Value [mM] (protein specific)
2
3
nitroethane
neutral form of substrate
Neurospora crassa
3.1
2-Nitropropane
anionic form of substrate
Neurospora crassa
3.1
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
6
2-Nitropropane
anionic form of substrate
Neurospora crassa
6
ethyl nitronate
anionic form of substrate
Neurospora crassa
8.3
1-Nitropropane
anionic form of substrate
Neurospora crassa
8.3
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
20
1-Nitropropane
neutral form of substrate
Neurospora crassa
23
nitroethane
neutral form of substrate
Neurospora crassa
33
2-Nitropropane
neutral form of substrate
Neurospora crassa
Molecular Weight [Da] (protein specific)
40000
2 * 40000, SDS-PAGE
Neurospora crassa
70000
gel filtration
Neurospora crassa
Purification (Commentary) (protein specific)
to homogeneity
Neurospora crassa
Source Tissue (protein specific)
mycelium
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
Substrates and Products (Substrate) (protein specific)
1-hydroxybutyl-2-nitronate + O2
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
?
1-hydroxyethyl-2-nitronate + O2
639232
Neurospora crassa
glycoaldehyde + HNO2
?
1-nitropentane + O2
3% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
?
1-nitropropane + O2
21% of the activity with 2-nitropropane
639232
Neurospora crassa
propionaldehyde + HNO2
?
2-hydroxypentyl-3-nitronate + O2
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
?
2-nitroethanol + O2
13% of the activity with 2-nitropropane
639232
Neurospora crassa
glycoaldehyde + HNO2
?
2-nitropropane + O2
639232
Neurospora crassa
acetone + HNO2
?
3-nitro-2-butanol + O2
14% of the activity with 2-nitropropane
639232
Neurospora crassa
3-hydroxy-butane-2-one + HNO2
?
3-nitro-2-pentanol + O2
20% of the activity with 2-nitropropane
639232
Neurospora crassa
2-hydroxy-pentane-3-one + HNO2
?
3-nitropropionic acid + O2
12% of the activity with 2-nitropropane
639232
Neurospora crassa
?
?
cyclohexyl nitronate + O2
639232
Neurospora crassa
? + HNO2
?
ethyl nitronate + O2
639232
Neurospora crassa
acetaldehyde + HNO2
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds
639232
Neurospora crassa
?
?
additional information
anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates
639232
Neurospora crassa
?
?
nitrocyclohexane + O2
2% of the activity with 2-nitropropane
639232
Neurospora crassa
? + HNO2
?
nitroethane + O2
27% of the activity with 2-nitropropane
639232
Neurospora crassa
acetaldehyde + HNO2
?
pentyl-1-nitronate + O2
639232
Neurospora crassa
? + HNO2
?
propionate-3-nitronate + O2
639232
Neurospora crassa
?
?
propyl-1-nitronate + O2
639232
Neurospora crassa
propionaldehyde + HNO2
?
propyl-2-nitronate + O2
639232
Neurospora crassa
acetone + HNO2
?
Subunits (protein specific)
dimer
2 * 40000, SDS-PAGE
Neurospora crassa
Temperature Optimum [°C] (protein specific)
30
assay at
Neurospora crassa
Turnover Number [1/s] (protein specific)
32
nitroethane
neutral form of substrate
Neurospora crassa
35
1-Nitropropane
neutral form of substrate
Neurospora crassa
160
2-Nitropropane
neutral form of substrate
Neurospora crassa
1300
1-Nitropropane
anionic form of substrate
Neurospora crassa
1300
propyl-1-nitronate
anionic form of substrate
Neurospora crassa
2000
nitroethane
anionic form of substrate
Neurospora crassa
2000
ethyl nitronate
anionic form of substrate
Neurospora crassa
6400
2-Nitropropane
anionic form of substrate
Neurospora crassa
6400
propyl-2-nitronate
anionic form of substrate
Neurospora crassa
pH Optimum (protein specific)
6.5
assay at
Neurospora crassa
Other publictions for EC 1.13.12.16
745219
Vercammen
Pseudomonas aeruginosa LysR P ...
Pseudomonas aeruginosa
J. Bacteriol.
197
1026-1039
2015
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-
1
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-
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1
-
3
-
-
-
-
-
-
-
-
1
-
5
-
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-
-
-
-
-
1
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-
-
1
1
-
1
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-
-
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1
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745306
Salvi
The combined structural and k ...
Pseudomonas aeruginosa
J. Biol. Chem.
289
23764-23775
2014
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1
1
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-
-
4
-
-
-
1
-
10
-
-
1
1
-
-
-
-
8
1
5
1
-
-
3
1
-
-
1
-
-
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-
-
1
1
1
-
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
8
1
1
-
-
3
1
-
-
-
-
3
3
-
6
6
727018
Smitherman
Evidence for a transient perox ...
Cyberlindnera saturnus
Biochemistry
52
2694-2704
2013
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-
-
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-
6
-
-
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-
-
3
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-
-
-
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2
-
1
2
-
-
5
1
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-
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-
-
-
-
-
-
-
-
6
-
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-
-
-
-
-
-
2
-
2
-
-
5
1
-
-
-
-
-
-
-
6
6
728077
Klinkenberg
Rv1894c is a novel hypoxia-ind ...
Mycobacterium tuberculosis
J. Infect. Dis.
207
1525-1534
2013
-
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1
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1
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1
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10
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1
-
-
-
-
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1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
726808
Francis
A novel activity for fungal ni ...
Cyberlindnera saturnus, Neurospora crassa
Arch. Biochem. Biophys.
521
84-89
2012
-
-
-
-
-
-
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3
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10
-
-
-
-
-
-
-
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3
-
4
2
-
-
3
2
-
-
-
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-
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-
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-
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-
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-
-
3
-
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-
-
-
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-
-
-
-
-
-
3
-
2
-
-
3
2
-
-
-
-
3
3
-
3
3
724175
Li
Crystal structure and site-dir ...
Streptomyces ansochromogenes
Biochem. Biophys. Res. Commun.
405
344-348
2011
-
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1
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii
Arch. Biochem. Biophys.
493
53-61
2009
-
-
-
-
-
-
-
1
-
3
-
13
-
6
-
-
-
3
-
-
-
-
16
1
3
3
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
3
-
13
-
-
-
-
-
-
-
-
16
1
3
-
-
-
3
-
-
-
-
-
-
-
-
-
696315
Francis
Inflated kinetic isotope effec ...
Neurospora crassa
Biochemistry
48
2403-2410
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684712
Mijatovic
Oxidation of alkyl nitronates ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
473
61-68
2008
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1
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1
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1
1
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6
-
-
1
-
-
2
-
-
8
-
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
-
2
-
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
6
6
685283
Francis
The nonoxidative conversion of ...
Neurospora crassa
Biochemistry
47
9136-9144
2008
-
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1
-
2
-
1
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
5
-
1
1
-
-
2
1
1
-
-
2
-
-
-
-
1
-
-
2
-
-
1
2
6
-
-
-
-
-
-
-
1
-
-
-
-
5
-
1
-
-
2
1
1
-
-
-
-
-
-
4
4
672094
Francis
Probing the chemical steps of ...
Neurospora crassa
Biochemistry
45
13889-13898
2006
-
-
-
-
-
-
1
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
2
1
-
-
5
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
11
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
5
1
1
-
-
-
-
-
-
7
7
674590
Ha
Crystal structure of 2-nitropr ...
Pseudomonas aeruginosa
J. Biol. Chem.
281
18660-18667
2006
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1
1
4
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1
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1
-
-
5
-
-
1
-
-
-
-
-
2
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
4
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656289
Francis
Involvement of a flavosemiquin ...
Neurospora crassa
J. Biol. Chem.
280
5195-5204
2005
-
-
3
-
-
-
-
11
-
2
4
1
-
7
-
-
3
-
-
-
5
1
33
2
1
2
-
-
28
2
1
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
11
-
2
4
1
-
-
-
3
-
-
5
1
33
2
2
-
-
28
2
1
-
-
-
-
-
-
10
10
639233
Gadda
Substrate specificity of a nit ...
Fusarium oxysporum
Arch. Biochem. Biophys.
363
309-313
1999
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
14
-
1
1
-
-
-
2
-
-
1
2
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
639232
Gorlatova
Purification, characterization ...
Neurospora crassa
Appl. Environ. Microbiol.
64
1029-1033
1998
-
-
-
-
-
-
8
10
-
-
2
-
-
10
-
-
2
-
-
1
1
-
20
1
1
1
-
-
9
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
15
-
10
-
-
2
-
-
-
-
2
-
1
1
-
20
1
1
-
-
9
1
-
-
-
-
-
-
-
-
-
698266
Balogh-Hergovich
Copper-mediated oxygenation of ...
Cyberlindnera mrakii
Inorg. Chem.
37
6535-6537
1998
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
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1996
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1
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8
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8
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639222
Kido
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2-Nitropropane dioxygenase fro ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
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1984
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21
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2
2
1
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2
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2
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-
2
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23
2
1
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4
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2
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2
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21
-
2
2
1
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2
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2
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23
2
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4
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639226
Kido
-
Characterization of primary ni ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
1361-1362
1984
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1
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1
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1
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4
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1
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1
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1
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1
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1
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4
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639227
Kido
Oxidation of anionic nitroalka ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
234
468-475
1984
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11
2
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1
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9
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1
1
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8
-
1
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1
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1
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1
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14
-
2
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1
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1
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8
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1
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639224
Kido
Properties of 2-nitropropane d ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
253
226-232
1978
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19
2
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11
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1
2
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2
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1
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1
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1
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20
-
2
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1
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2
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639225
Kido
Purification and properties of ...
Fusarium oxysporum
J. Bacteriol.
133
53-58
1978
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10
6
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2
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4
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2
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1
1
1
14
1
1
2
2
6
-
1
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1
3
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3
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11
-
6
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2
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2
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1
1
1
14
1
2
2
6
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1
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1
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639223
Kido
A new oxygenase, 2-nitropropan ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
251
6994-7000
1976
3
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3
13
7
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1
4
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8
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1
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17
1
1
1
1
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2
2
-
2
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3
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2
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3
-
18
-
7
-
1
4
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1
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17
1
1
1
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2
2
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639235
Kido
Purification and properties of ...
Cyberlindnera mrakii
J. Bacteriol.
126
1261-1265
1976
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5
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1
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1
1
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5
1
1
1
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2
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1
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1
1
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5
1
1
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2
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