Reference to 1.13.12.16; Id = 639232 - BRENDA Enzyme Database

2-mercaptoethanol

1 mM, relative activity remaining 2%

Neurospora crassa

cytochrome c

0.04 mM, relative activity remaining 34%

Neurospora crassa

dithiothreitol

1 mM, complete inhibition

Neurospora crassa

EDTA

1 mM, relative activity remaining 95%

Neurospora crassa

GSH

5 mM, relative activity remaining 28%

Neurospora crassa

iodoacetate

1 mM, complete inhibition

Neurospora crassa

Nitro blue tetrazolium

2.5 mM, relative activity remaining 10%

Neurospora crassa

Superoxide dismutase

complete inhibition

Neurospora crassa

2

3

nitroethane

neutral form of substrate

Neurospora crassa

3.1

-

2-Nitropropane

anionic form of substrate

Neurospora crassa

3.1

-

propyl-2-nitronate

anionic form of substrate

Neurospora crassa

6

-

2-Nitropropane

anionic form of substrate

Neurospora crassa

6

-

ethyl nitronate

anionic form of substrate

Neurospora crassa

8.3

-

1-Nitropropane

anionic form of substrate

Neurospora crassa

8.3

-

propyl-1-nitronate

anionic form of substrate

Neurospora crassa

20

-

1-Nitropropane

neutral form of substrate

Neurospora crassa

23

-

nitroethane

neutral form of substrate

Neurospora crassa

33

-

2-Nitropropane

neutral form of substrate

Neurospora crassa

40000

-

2 * 40000, SDS-PAGE

Neurospora crassa

70000

-

gel filtration

Neurospora crassa

-

Neurospora crassa

-

ATCC 10337

-

Neurospora crassa

to homogeneity

Neurospora crassa

mycelium

-

Neurospora crassa

-

10000

-

Neurospora crassa

1-hydroxybutyl-2-nitronate + O2

-

639232

Neurospora crassa

3-hydroxy-butane-2-one + HNO2

-

?

1-hydroxyethyl-2-nitronate + O2

-

639232

Neurospora crassa

glycoaldehyde + HNO2

-

?

1-nitropentane + O2

3% of the activity with 2-nitropropane

639232

Neurospora crassa

? + HNO2

-

?

1-nitropropane + O2

21% of the activity with 2-nitropropane

639232

Neurospora crassa

propionaldehyde + HNO2

-

?

2-hydroxypentyl-3-nitronate + O2

-

639232

Neurospora crassa

2-hydroxy-pentane-3-one + HNO2

-

?

2-nitroethanol + O2

13% of the activity with 2-nitropropane

639232

Neurospora crassa

glycoaldehyde + HNO2

-

?

2-nitropropane + O2

-

639232

Neurospora crassa

acetone + HNO2

-

?

3-nitro-2-butanol + O2

14% of the activity with 2-nitropropane

639232

Neurospora crassa

3-hydroxy-butane-2-one + HNO2

-

?

3-nitro-2-pentanol + O2

20% of the activity with 2-nitropropane

639232

Neurospora crassa

2-hydroxy-pentane-3-one + HNO2

-

?

3-nitropropionic acid + O2

12% of the activity with 2-nitropropane

639232

Neurospora crassa

?

-

?

cyclohexyl nitronate + O2

-

639232

Neurospora crassa

? + HNO2

-

?

ethyl nitronate + O2

-

639232

Neurospora crassa

acetaldehyde + HNO2

-

?

additional information

anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds

639232

Neurospora crassa

?

-

?

additional information

anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates

639232

Neurospora crassa

?

-

?

nitrocyclohexane + O2

2% of the activity with 2-nitropropane

639232

Neurospora crassa

? + HNO2

-

?

nitroethane + O2

27% of the activity with 2-nitropropane

639232

Neurospora crassa

acetaldehyde + HNO2

-

?

pentyl-1-nitronate + O2

-

639232

Neurospora crassa

? + HNO2

-

?

propionate-3-nitronate + O2

-

639232

Neurospora crassa

?

-

?

propyl-1-nitronate + O2

-

639232

Neurospora crassa

propionaldehyde + HNO2

-

?

propyl-2-nitronate + O2

-

639232

Neurospora crassa

acetone + HNO2

-

?

dimer

2 * 40000, SDS-PAGE

Neurospora crassa

2-nitropropane dioxygenase

-

Neurospora crassa

30

-

assay at

Neurospora crassa

32

-

nitroethane

neutral form of substrate

Neurospora crassa

35

-

1-Nitropropane

neutral form of substrate

Neurospora crassa

160

-

2-Nitropropane

neutral form of substrate

Neurospora crassa

1300

-

1-Nitropropane

anionic form of substrate

Neurospora crassa

1300

-

propyl-1-nitronate

anionic form of substrate

Neurospora crassa

2000

-

nitroethane

anionic form of substrate

Neurospora crassa

2000

-

ethyl nitronate

anionic form of substrate

Neurospora crassa

6400

-

2-Nitropropane

anionic form of substrate

Neurospora crassa

6400

-

propyl-2-nitronate

anionic form of substrate

Neurospora crassa

6.5

-

assay at

Neurospora crassa

FMN

2 mol of FMN per mol of subunit

Neurospora crassa

FMN

dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit

Neurospora crassa

FMN

2 mol of FMN per mol of subunit

Neurospora crassa

FMN

dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit

Neurospora crassa

2-mercaptoethanol

-

Neurospora crassa

2-mercaptoethanol

1 mM, relative activity remaining 2%

Neurospora crassa

cytochrome c

-

Neurospora crassa

cytochrome c

0.04 mM, relative activity remaining 34%

Neurospora crassa

dithiothreitol

-

Neurospora crassa

dithiothreitol

1 mM, complete inhibition

Neurospora crassa

EDTA

1 mM, relative activity remaining 95%

Neurospora crassa

GSH

-

Neurospora crassa

GSH

5 mM, relative activity remaining 28%

Neurospora crassa

iodoacetate

-

Neurospora crassa

iodoacetate

1 mM, complete inhibition

Neurospora crassa

Nitro blue tetrazolium

-

Neurospora crassa

Nitro blue tetrazolium

2.5 mM, relative activity remaining 10%

Neurospora crassa

Superoxide dismutase

-

Neurospora crassa

Superoxide dismutase

complete inhibition

Neurospora crassa

2

3

nitroethane

neutral form of substrate

Neurospora crassa

3.1

-

2-Nitropropane

anionic form of substrate

Neurospora crassa

3.1

-

propyl-2-nitronate

anionic form of substrate

Neurospora crassa

6

-

2-Nitropropane

anionic form of substrate

Neurospora crassa

6

-

ethyl nitronate

anionic form of substrate

Neurospora crassa

8.3

-

1-Nitropropane

anionic form of substrate

Neurospora crassa

8.3

-

propyl-1-nitronate

anionic form of substrate

Neurospora crassa

20

-

1-Nitropropane

neutral form of substrate

Neurospora crassa

23

-

nitroethane

neutral form of substrate

Neurospora crassa

33

-

2-Nitropropane

neutral form of substrate

Neurospora crassa

40000

-

2 * 40000, SDS-PAGE

Neurospora crassa

70000

-

gel filtration

Neurospora crassa

-

Neurospora crassa

to homogeneity

Neurospora crassa

mycelium

-

Neurospora crassa

-

10000

-

Neurospora crassa

1-hydroxybutyl-2-nitronate + O2

-

639232

Neurospora crassa

3-hydroxy-butane-2-one + HNO2

-

?

1-hydroxyethyl-2-nitronate + O2

-

639232

Neurospora crassa

glycoaldehyde + HNO2

-

?

1-nitropentane + O2

3% of the activity with 2-nitropropane

639232

Neurospora crassa

? + HNO2

-

?

1-nitropropane + O2

21% of the activity with 2-nitropropane

639232

Neurospora crassa

propionaldehyde + HNO2

-

?

2-hydroxypentyl-3-nitronate + O2

-

639232

Neurospora crassa

2-hydroxy-pentane-3-one + HNO2

-

?

2-nitroethanol + O2

13% of the activity with 2-nitropropane

639232

Neurospora crassa

glycoaldehyde + HNO2

-

?

2-nitropropane + O2

-

639232

Neurospora crassa

acetone + HNO2

-

?

3-nitro-2-butanol + O2

14% of the activity with 2-nitropropane

639232

Neurospora crassa

3-hydroxy-butane-2-one + HNO2

-

?

3-nitro-2-pentanol + O2

20% of the activity with 2-nitropropane

639232

Neurospora crassa

2-hydroxy-pentane-3-one + HNO2

-

?

3-nitropropionic acid + O2

12% of the activity with 2-nitropropane

639232

Neurospora crassa

?

-

?

cyclohexyl nitronate + O2

-

639232

Neurospora crassa

? + HNO2

-

?

ethyl nitronate + O2

-

639232

Neurospora crassa

acetaldehyde + HNO2

-

?

additional information

anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds

639232

Neurospora crassa

?

-

?

additional information

anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates

639232

Neurospora crassa

?

-

?

nitrocyclohexane + O2

2% of the activity with 2-nitropropane

639232

Neurospora crassa

? + HNO2

-

?

nitroethane + O2

27% of the activity with 2-nitropropane

639232

Neurospora crassa

acetaldehyde + HNO2

-

?

pentyl-1-nitronate + O2

-

639232

Neurospora crassa

? + HNO2

-

?

propionate-3-nitronate + O2

-

639232

Neurospora crassa

?

-

?

propyl-1-nitronate + O2

-

639232

Neurospora crassa

propionaldehyde + HNO2

-

?

propyl-2-nitronate + O2

-

639232

Neurospora crassa

acetone + HNO2

-

?

dimer

2 * 40000, SDS-PAGE

Neurospora crassa

30

-

assay at

Neurospora crassa

32

-

nitroethane

neutral form of substrate

Neurospora crassa

35

-

1-Nitropropane

neutral form of substrate

Neurospora crassa

160

-

2-Nitropropane

neutral form of substrate

Neurospora crassa

1300

-

1-Nitropropane

anionic form of substrate

Neurospora crassa

1300

-

propyl-1-nitronate

anionic form of substrate

Neurospora crassa

2000

-

nitroethane

anionic form of substrate

Neurospora crassa

2000

-

ethyl nitronate

anionic form of substrate

Neurospora crassa

6400

-

2-Nitropropane

anionic form of substrate

Neurospora crassa

6400

-

propyl-2-nitronate

anionic form of substrate

Neurospora crassa

6.5

-

assay at

Neurospora crassa

745219

Vercammen

Pseudomonas aeruginosa LysR P ...

Pseudomonas aeruginosa

J. Bacteriol.

197

1026-1039

2015

-

1

-

1

-

1

-

3

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

2

-

745306

Salvi

The combined structural and k ...

Pseudomonas aeruginosa

J. Biol. Chem.

289

23764-23775

2014

-

1

-

4

-

1

-

10

-

1

-

8

1

5

1

-

3

1

-

1

-

1

-

4

-

1

-

1

-

8

1

-

3

1

-

3

-

6

727018

Smitherman

Evidence for a transient perox ...

Cyberlindnera saturnus

Biochemistry

52

2694-2704

2013

-

6

-

3

-

2

-

1

2

-

5

1

-

6

-

2

-

2

-

5

1

-

6

728077

Klinkenberg

Rv1894c is a novel hypoxia-ind ...

Mycobacterium tuberculosis

J. Infect. Dis.

207

1525-1534

2013

-

1

-

1

-

1

-

10

-

1

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

726808

Francis

A novel activity for fungal ni ...

Cyberlindnera saturnus, Neurospora crassa

Arch. Biochem. Biophys.

521

84-89

2012

-

3

-

10

-

3

-

4

2

-

3

2

-

3

-

3

-

2

-

3

2

-

3

-

3

724175

Li

Crystal structure and site-dir ...

Streptomyces ansochromogenes

Biochem. Biophys. Res. Commun.

405

344-348

2011

-

1

3

-

2

-

3

-

2

-

1

-

1

-

1

3

-

3

-

1

-

695891

Gadda

Nitronate monooxygenase, a mod ...

Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii

Arch. Biochem. Biophys.

493

53-61

2009

-

1

-

3

-

13

-

6

-

3

-

16

1

3

-

3

-

3

-

3

-

1

-

3

-

13

-

16

1

3

-

3

-

696315

Francis

Inflated kinetic isotope effec ...

Neurospora crassa

Biochemistry

48

2403-2410

2009

-

6

-

1

-

1

-

684712

Mijatovic

Oxidation of alkyl nitronates ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

473

61-68

2008

-

1

-

1

-

1

-

6

-

1

-

2

-

8

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

8

-

1

-

2

-

6

685283

Francis

The nonoxidative conversion of ...

Neurospora crassa

Biochemistry

47

9136-9144

2008

-

1

-

2

-

1

6

-

4

-

1

-

5

-

1

-

2

1

-

2

-

1

-

2

-

1

2

6

-

1

-

5

-

1

-

2

1

-

4

672094

Francis

Probing the chemical steps of ...

Neurospora crassa

Biochemistry

45

13889-13898

2006

-

1

11

-

1

-

1

-

4

-

2

1

-

5

1

-

1

-

1

-

1

-

11

-

1

-

4

-

1

-

5

1

-

7

674590

Ha

Crystal structure of 2-nitropr ...

Pseudomonas aeruginosa

J. Biol. Chem.

281

18660-18667

2006

-

1

4

-

1

-

1

-

5

-

1

-

2

1

-

1

-

1

-

1

4

-

1

-

1

-

1

-

2

1

-

1

-

656289

Francis

Involvement of a flavosemiquin ...

Neurospora crassa

J. Biol. Chem.

280

5195-5204

2005

-

3

-

11

-

2

4

1

-

7

-

3

-

5

1

33

2

1

2

-

28

2

1

-

3

-

3

-

11

-

2

4

1

-

3

-

5

1

33

2

-

28

2

1

-

10

639233

Gadda

Substrate specificity of a nit ...

Fusarium oxysporum

Arch. Biochem. Biophys.

363

309-313

1999

-

1

-

1

-

3

-

14

-

1

-

2

-

1

2

-

1

-

2

-

1

-

14

-

1

-

2

-

639232

Gorlatova

Purification, characterization ...

Neurospora crassa

Appl. Environ. Microbiol.

64

1029-1033

1998

-

8

10

-

2

-

10

-

2

-

1

-

20

1

-

9

1

-

2

-

2

-

15

-

10

-

2

-

2

-

1

-

20

1

-

9

1

-

698266

Balogh-Hergovich

Copper-mediated oxygenation of ...

Cyberlindnera mrakii

Inorg. Chem.

37

6535-6537

1998

-

1

-

1

-

1

-

1

-

1

-

1

-

697294

Balogh-Hergovich

-

Copper mediated conversion of ...

Cyberlindnera mrakii

Chem. Lett.

25

573-574

1996

-

1

-

8

-

8

-

639222

Kido

-

2-Nitropropane dioxygenase fro ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

2549-2554

1984

-

21

-

2

1

-

2

-

2

-

2

-

23

2

1

-

4

-

2

-

2

-

21

-

2

1

-

2

-

2

-

23

2

-

4

-

639226

Kido

-

Characterization of primary ni ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

1361-1362

1984

-

1

-

1

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

4

-

639227

Kido

Oxidation of anionic nitroalka ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

234

468-475

1984

-

11

2

-

1

-

9

-

1

-

8

-

1

-

1

-

1

-

1

-

14

-

2

-

1

-

1

-

8

-

1

-

639224

Kido

Properties of 2-nitropropane d ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

253

226-232

1978

-

19

2

-

11

-

1

2

-

2

-

1

-

1

-

1

-

20

-

2

-

1

-

2

-

639225

Kido

Purification and properties of ...

Fusarium oxysporum

J. Bacteriol.

133

53-58

1978

-

10

6

-

2

-

4

-

2

-

1

14

1

2

6

-

1

-

1

3

-

3

-

11

-

6

-

2

-

2

-

1

14

1

2

6

-

1

-

1

-

639223

Kido

A new oxygenase, 2-nitropropan ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

251

6994-7000

1976

3

-

3

13

7

-

1

4

-

8

-

1

-

17

1

-

2

-

2

-

3

-

2

-

3

-

18

-

7

-

1

4

-

1

-

17

1

-

2

-

639235

Kido

Purification and properties of ...

Cyberlindnera mrakii

J. Bacteriol.

126

1261-1265

1976

-

5

-

1

-

1

-

5

1

-

2

-

1

-

1

-

5

1

-

2

-

Purification, characterization, and mechanism of a flavin mononucleotide-dependent 2-nitropropane dioxygenase from Neurospora crassa

Data extracted from this reference: