BRENDA - Enzyme Database
show all sequences of 1.13.12.16

Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzes by 2-nitropropane dioxygenase

Francis, K.; Russell, B.; Gadda, G.; J. Biol. Chem. 280, 5195-5204 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Neurospora crassa
gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue
Neurospora crassa
gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue; expressed in Escherichia coli BL21(DE3) cells
Neurospora crassa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, steady-state kinetic mechanism
Neurospora crassa
1.4
-
1-nitrohexane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.4
-
hexyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
3.1
-
ethyl nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
5.5
-
propyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
7.1
-
1-nitropentane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
10
-
butyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
15
-
1-nitrobutane
in 50 mM Tris-Cl, pH 8 at 30°C
Neurospora crassa
17.9
-
1-nitrobutane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
24
-
1-Nitropropane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
29
-
nitroethane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
stoichiometry of 0.02 FMN per 1 monomer of enzyme
Neurospora crassa
additional information
devoid of iron
Neurospora crassa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39920
-
calculated
Neurospora crassa
39920
-
calculated from the amino acid composition of the enzyme
Neurospora crassa
40000
-
SDS-PAGE
Neurospora crassa
80000
-
recombinant enzyme, gel filtration
Neurospora crassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Neurospora crassa
enzyme is more specific for nitronates than nitroalkanes
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Neurospora crassa
Q01284
-
-
Neurospora crassa
Q01284
gene ncd-2
-
Purification (Commentary)
Purification (Commentary)
Organism
DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography
Neurospora crassa
DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography; recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography
Neurospora crassa
recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography
Neurospora crassa
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13
-
cell free extract
Neurospora crassa
49
-
after DEAE-Sepharose column chromatography purification only
Neurospora crassa
80
-
at 25°C assay temperature, purified recombinant enzyme, substrate ethyl nitronate
Neurospora crassa
80
-
after DEAE-Sepharose column chromatography and octyl-Sepharose column chromatography purification
Neurospora crassa
80
-
after DEAE-Sepharose column chromatography and octyl-Sepharose column chromatography purification; at 25°C assay temperature, purified recombinant enzyme, substrate ethyl nitronate
Neurospora crassa
Storage Stability
Storage Stability
Organism
-20°C, 50 mM potassium phosphate, pH 7.4, 6 months, no loss of activity
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-nitrobutane + O2
-
656289
Neurospora crassa
butyraldehyde + HNO2
-
-
-
?
1-nitrobutane + O2
neutral and anionic form
656289
Neurospora crassa
butyraldehyde + HNO2
-
-
-
?
1-nitrobutane + O2
-
656289
Neurospora crassa
butyraldehyde + nitrite
-
-
-
?
1-nitrohexane + O2
-
656289
Neurospora crassa
hexanaldehyde + HNO2
-
-
-
?
1-nitrohexane + O2
neutral and anionic form
656289
Neurospora crassa
hexanaldehyde + HNO2
-
-
-
?
1-nitrohexane + O2
-
656289
Neurospora crassa
hexanaldehyde + nitrite
-
-
-
?
1-nitropentane + O2
-
656289
Neurospora crassa
pentanaldehyde + HNO2
-
-
-
?
1-nitropentane + O2
neutral and anionic form
656289
Neurospora crassa
pentanaldehyde + HNO2
-
-
-
?
1-nitropentane + O2
-
656289
Neurospora crassa
pentanaldehyde + nitrite
-
-
-
?
1-nitropropane + O2
-
656289
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
1-nitropropane + O2
neutral and anionic form
656289
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
1-nitropropane + O2
-
656289
Neurospora crassa
propionaldehyde + nitrite
-
-
-
?
2-nitropropane + O2
-
656289
Neurospora crassa
acetone + HNO2
-
-
-
?
2-nitropropane + O2
neutral and anionic form
656289
Neurospora crassa
acetone + HNO2
-
-
-
?
2-nitropropane + O2
-
656289
Neurospora crassa
acetone + nitrite
-
-
-
?
butyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
butyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
ethyl nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
ethylnitronate + O2
neutral and anionic form
656289
Neurospora crassa
acetaldehyde + HNO2
-
-
-
?
hexyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
hexyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
additional information
the reduced enzyme can reduce the substrate under anaerobically conditions, substrate specificity with nitroalkanes and alkyl nitronates, O2 is delivered from air-saturated buffer in the assay reaction, enzyme catalyzes the 2-step oxidative denitrification of nitroalkanes to their corresponding carbonyl compounds and nitrite
656289
Neurospora crassa
?
-
-
-
?
additional information
enzyme is more specific for nitronates than nitroalkanes
656289
Neurospora crassa
?
-
-
-
?
nitroethane + O2
-
656289
Neurospora crassa
ethanal + nitrite
-
-
-
?
nitroethane + O2
neutral and anionic form
656289
Neurospora crassa
ethanal + nitrite
-
-
-
?
nitroethane + O2 + H2O
-
656289
Neurospora crassa
ethanal + nitrite + H2O2
-
-
-
?
pentyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
pentyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-1-nitronate + O2
-
656289
Neurospora crassa
?
-
-
-
?
propyl-2-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-2-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, recombinant enzyme, SDS-PAGE
Neurospora crassa
homodimer
2 * 40000, gel filtration, heat denaturation, and mass spectroscopic analysis
Neurospora crassa
Synonyms
Synonyms
Commentary
Organism
2-nitropropane dioxygenase
-
Neurospora crassa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Neurospora crassa
30
-
assay at
Neurospora crassa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kcat at different conditions, overview, effects of presence of superoxide dimutase or catalase on the turnover rate of the enzyme
Neurospora crassa
0.82
-
2-Nitropropane
pH 8.0, 30°C
Neurospora crassa
1.3
-
1-nitrohexane
pH 8.0, 30°C
Neurospora crassa
1.5
-
1-nitrohexane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.7
-
1-nitrohexane
pH 8.0, 30°C
Neurospora crassa
3
-
1-nitropentane
pH 8.0, 30°C
Neurospora crassa
3.5
-
nitroethane
pH 8.0, 30°C
Neurospora crassa
3.6
-
1-nitrobutane
pH 8.0, 30°C
Neurospora crassa
4.3
-
1-nitropentane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
5.9
-
1-nitrobutane
pH 8.0, 30°C
Neurospora crassa
6.2
-
1-nitrobutane
in 50 mM Tris-Cl, pH 8 at 30°C
Neurospora crassa
8.3
-
1-Nitropropane
pH 8.0, 30°C
Neurospora crassa
11
-
nitroethane
pH 8.0, 30°C
Neurospora crassa
14
-
nitroethane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
15
-
propyl-2-nitronate
pH 8.0, 30°C
Neurospora crassa
20
-
1-Nitropropane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
21
-
hexyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
22
-
hexyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
29
-
propyl-2-nitronate
pH 8.0, 30°C
Neurospora crassa
35
-
pentyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
40
-
ethyl nitronate
pH 8.0, 30°C
Neurospora crassa
42
-
propyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
42
-
butyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
51
-
ethyl nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
55
-
butyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
56
-
butyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
57
-
ethylnitronate
pH 8.0, 30°C
Neurospora crassa
60
-
propyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Neurospora crassa
8
-
assay at
Neurospora crassa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
-
Neurospora crassa
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
dependent on, stoichiometry of 0.84 FMN per 1 monomer of enzyme, noncovalently bound
Neurospora crassa
FMN
contains a mol of non-covalently bound FMN per mol of subunit, contains 0.84 FMN per monomer of enzyme
Neurospora crassa
FMN
dependent on, stoichiometry of 0.84 FMN per 1 monomer of enzyme, noncovalently bound, contains a mol of non-covalently bound FMN per mol of subunit, contains 0.84 FMN per monomer of enzyme
Neurospora crassa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Neurospora crassa
gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue
Neurospora crassa
gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue; expressed in Escherichia coli BL21(DE3) cells
Neurospora crassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
dependent on, stoichiometry of 0.84 FMN per 1 monomer of enzyme, noncovalently bound
Neurospora crassa
FMN
contains a mol of non-covalently bound FMN per mol of subunit, contains 0.84 FMN per monomer of enzyme
Neurospora crassa
FMN
dependent on, stoichiometry of 0.84 FMN per 1 monomer of enzyme, noncovalently bound, contains a mol of non-covalently bound FMN per mol of subunit, contains 0.84 FMN per monomer of enzyme
Neurospora crassa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, steady-state kinetic mechanism
Neurospora crassa
1.4
-
1-nitrohexane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.4
-
hexyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
3.1
-
ethyl nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
5.5
-
propyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
7.1
-
1-nitropentane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
10
-
butyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
15
-
1-nitrobutane
in 50 mM Tris-Cl, pH 8 at 30°C
Neurospora crassa
17.9
-
1-nitrobutane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
24
-
1-Nitropropane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
29
-
nitroethane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
stoichiometry of 0.02 FMN per 1 monomer of enzyme
Neurospora crassa
additional information
devoid of iron
Neurospora crassa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39920
-
calculated
Neurospora crassa
39920
-
calculated from the amino acid composition of the enzyme
Neurospora crassa
40000
-
SDS-PAGE
Neurospora crassa
80000
-
recombinant enzyme, gel filtration
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Neurospora crassa
enzyme is more specific for nitronates than nitroalkanes
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography
Neurospora crassa
DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography; recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography
Neurospora crassa
recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13
-
cell free extract
Neurospora crassa
49
-
after DEAE-Sepharose column chromatography purification only
Neurospora crassa
80
-
at 25°C assay temperature, purified recombinant enzyme, substrate ethyl nitronate
Neurospora crassa
80
-
after DEAE-Sepharose column chromatography and octyl-Sepharose column chromatography purification
Neurospora crassa
80
-
after DEAE-Sepharose column chromatography and octyl-Sepharose column chromatography purification; at 25°C assay temperature, purified recombinant enzyme, substrate ethyl nitronate
Neurospora crassa
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 50 mM potassium phosphate, pH 7.4, 6 months, no loss of activity
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-nitrobutane + O2
-
656289
Neurospora crassa
butyraldehyde + HNO2
-
-
-
?
1-nitrobutane + O2
neutral and anionic form
656289
Neurospora crassa
butyraldehyde + HNO2
-
-
-
?
1-nitrobutane + O2
-
656289
Neurospora crassa
butyraldehyde + nitrite
-
-
-
?
1-nitrohexane + O2
-
656289
Neurospora crassa
hexanaldehyde + HNO2
-
-
-
?
1-nitrohexane + O2
neutral and anionic form
656289
Neurospora crassa
hexanaldehyde + HNO2
-
-
-
?
1-nitrohexane + O2
-
656289
Neurospora crassa
hexanaldehyde + nitrite
-
-
-
?
1-nitropentane + O2
-
656289
Neurospora crassa
pentanaldehyde + HNO2
-
-
-
?
1-nitropentane + O2
neutral and anionic form
656289
Neurospora crassa
pentanaldehyde + HNO2
-
-
-
?
1-nitropentane + O2
-
656289
Neurospora crassa
pentanaldehyde + nitrite
-
-
-
?
1-nitropropane + O2
-
656289
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
1-nitropropane + O2
neutral and anionic form
656289
Neurospora crassa
propionaldehyde + HNO2
-
-
-
?
1-nitropropane + O2
-
656289
Neurospora crassa
propionaldehyde + nitrite
-
-
-
?
2-nitropropane + O2
-
656289
Neurospora crassa
acetone + HNO2
-
-
-
?
2-nitropropane + O2
neutral and anionic form
656289
Neurospora crassa
acetone + HNO2
-
-
-
?
2-nitropropane + O2
-
656289
Neurospora crassa
acetone + nitrite
-
-
-
?
butyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
butyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
ethyl nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
ethylnitronate + O2
neutral and anionic form
656289
Neurospora crassa
acetaldehyde + HNO2
-
-
-
?
hexyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
hexyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
additional information
the reduced enzyme can reduce the substrate under anaerobically conditions, substrate specificity with nitroalkanes and alkyl nitronates, O2 is delivered from air-saturated buffer in the assay reaction, enzyme catalyzes the 2-step oxidative denitrification of nitroalkanes to their corresponding carbonyl compounds and nitrite
656289
Neurospora crassa
?
-
-
-
?
additional information
enzyme is more specific for nitronates than nitroalkanes
656289
Neurospora crassa
?
-
-
-
?
nitroethane + O2
-
656289
Neurospora crassa
ethanal + nitrite
-
-
-
?
nitroethane + O2
neutral and anionic form
656289
Neurospora crassa
ethanal + nitrite
-
-
-
?
nitroethane + O2 + H2O
-
656289
Neurospora crassa
ethanal + nitrite + H2O2
-
-
-
?
pentyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
pentyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-1-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-1-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-1-nitronate + O2
-
656289
Neurospora crassa
?
-
-
-
?
propyl-2-nitronate + O2
-
656289
Neurospora crassa
? + nitrite
-
-
-
?
propyl-2-nitronate + O2
neutral and anionic form
656289
Neurospora crassa
? + nitrite
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, recombinant enzyme, SDS-PAGE
Neurospora crassa
homodimer
2 * 40000, gel filtration, heat denaturation, and mass spectroscopic analysis
Neurospora crassa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Neurospora crassa
30
-
assay at
Neurospora crassa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kcat at different conditions, overview, effects of presence of superoxide dimutase or catalase on the turnover rate of the enzyme
Neurospora crassa
0.82
-
2-Nitropropane
pH 8.0, 30°C
Neurospora crassa
1.3
-
1-nitrohexane
pH 8.0, 30°C
Neurospora crassa
1.5
-
1-nitrohexane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.7
-
1-nitrohexane
pH 8.0, 30°C
Neurospora crassa
3
-
1-nitropentane
pH 8.0, 30°C
Neurospora crassa
3.5
-
nitroethane
pH 8.0, 30°C
Neurospora crassa
3.6
-
1-nitrobutane
pH 8.0, 30°C
Neurospora crassa
4.3
-
1-nitropentane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
5.9
-
1-nitrobutane
pH 8.0, 30°C
Neurospora crassa
6.2
-
1-nitrobutane
in 50 mM Tris-Cl, pH 8 at 30°C
Neurospora crassa
8.3
-
1-Nitropropane
pH 8.0, 30°C
Neurospora crassa
11
-
nitroethane
pH 8.0, 30°C
Neurospora crassa
14
-
nitroethane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
15
-
propyl-2-nitronate
pH 8.0, 30°C
Neurospora crassa
20
-
1-Nitropropane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
21
-
hexyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
22
-
hexyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
29
-
propyl-2-nitronate
pH 8.0, 30°C
Neurospora crassa
35
-
pentyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
40
-
ethyl nitronate
pH 8.0, 30°C
Neurospora crassa
42
-
propyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
42
-
butyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
51
-
ethyl nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
55
-
butyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
56
-
butyl-1-nitronate
pH 8.0, 30°C
Neurospora crassa
57
-
ethylnitronate
pH 8.0, 30°C
Neurospora crassa
60
-
propyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Neurospora crassa
8
-
assay at
Neurospora crassa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
-
Neurospora crassa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.35
-
1-nitrobutane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
0.48
-
nitroethane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
0.61
-
1-nitropentane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
0.83
-
1-Nitropropane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.1
-
1-nitrohexane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.2
-
pentyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
5.6
-
butyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
11.4
-
propyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
16
-
hexyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
16.5
-
ethyl nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.35
-
1-nitrobutane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
0.48
-
nitroethane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
0.61
-
1-nitropentane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
0.83
-
1-Nitropropane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.1
-
1-nitrohexane
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
1.2
-
pentyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
5.6
-
butyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
11.4
-
propyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
16
-
hexyl-1-nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
16.5
-
ethyl nitronate
in 50 mM Tris-HCl, pH 8 at 30°C
Neurospora crassa
Other publictions for EC 1.13.12.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745219
Vercammen
Pseudomonas aeruginosa LysR P ...
Pseudomonas aeruginosa
J. Bacteriol.
197
1026-1039
2015
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-
1
-
1
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1
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3
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1
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5
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1
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1
1
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1
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1
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-
1
-
-
-
-
-
-
-
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2
2
-
-
-
745306
Salvi
The combined structural and k ...
Pseudomonas aeruginosa
J. Biol. Chem.
289
23764-23775
2014
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-
1
1
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4
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1
-
10
-
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1
1
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8
1
5
1
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3
1
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1
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1
1
1
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-
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-
4
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1
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1
-
-
-
-
8
1
1
-
-
3
1
-
-
-
-
3
3
-
6
6
727018
Smitherman
Evidence for a transient perox ...
Cyberlindnera saturnus
Biochemistry
52
2694-2704
2013
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-
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6
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3
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2
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1
2
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5
1
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6
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2
-
2
-
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5
1
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6
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728077
Klinkenberg
Rv1894c is a novel hypoxia-ind ...
Mycobacterium tuberculosis
J. Infect. Dis.
207
1525-1534
2013
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1
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1
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2
1
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1
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1
1
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1
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1
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-
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-
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-
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1
-
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-
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1
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
726808
Francis
A novel activity for fungal ni ...
Cyberlindnera saturnus, Neurospora crassa
Arch. Biochem. Biophys.
521
84-89
2012
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-
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3
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10
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3
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4
2
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3
2
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3
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-
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-
-
-
3
-
2
-
-
3
2
-
-
-
-
3
3
-
3
3
724175
Li
Crystal structure and site-dir ...
Streptomyces ansochromogenes
Biochem. Biophys. Res. Commun.
405
344-348
2011
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1
1
3
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2
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-
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3
-
2
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1
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1
-
1
3
-
-
-
-
-
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-
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-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii
Arch. Biochem. Biophys.
493
53-61
2009
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-
-
-
-
-
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1
-
3
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13
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6
-
-
-
3
-
-
-
-
16
1
3
3
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
3
-
13
-
-
-
-
-
-
-
-
16
1
3
-
-
-
3
-
-
-
-
-
-
-
-
-
696315
Francis
Inflated kinetic isotope effec ...
Neurospora crassa
Biochemistry
48
2403-2410
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
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-
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-
-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684712
Mijatovic
Oxidation of alkyl nitronates ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
473
61-68
2008
-
-
1
-
-
-
1
-
-
1
1
-
-
6
-
-
1
-
-
2
-
-
8
-
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
-
2
-
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
6
6
685283
Francis
The nonoxidative conversion of ...
Neurospora crassa
Biochemistry
47
9136-9144
2008
-
-
1
-
2
-
1
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
5
-
1
1
-
-
2
1
1
-
-
2
-
-
-
-
1
-
-
2
-
-
1
2
6
-
-
-
-
-
-
-
1
-
-
-
-
5
-
1
-
-
2
1
1
-
-
-
-
-
-
4
4
672094
Francis
Probing the chemical steps of ...
Neurospora crassa
Biochemistry
45
13889-13898
2006
-
-
-
-
-
-
1
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
2
1
-
-
5
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
11
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
5
1
1
-
-
-
-
-
-
7
7
674590
Ha
Crystal structure of 2-nitropr ...
Pseudomonas aeruginosa
J. Biol. Chem.
281
18660-18667
2006
-
-
1
1
4
-
1
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
2
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
4
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656289
Francis
Involvement of a flavosemiquin ...
Neurospora crassa
J. Biol. Chem.
280
5195-5204
2005
-
-
3
-
-
-
-
11
-
2
4
1
-
7
-
-
3
-
-
-
5
1
33
2
1
2
-
-
28
2
1
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
11
-
2
4
1
-
-
-
3
-
-
5
1
33
2
2
-
-
28
2
1
-
-
-
-
-
-
10
10
639233
Gadda
Substrate specificity of a nit ...
Fusarium oxysporum
Arch. Biochem. Biophys.
363
309-313
1999
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
14
-
1
1
-
-
-
2
-
-
1
2
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
639232
Gorlatova
Purification, characterization ...
Neurospora crassa
Appl. Environ. Microbiol.
64
1029-1033
1998
-
-
-
-
-
-
8
10
-
-
2
-
-
10
-
-
2
-
-
1
1
-
20
1
1
1
-
-
9
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
15
-
10
-
-
2
-
-
-
-
2
-
1
1
-
20
1
1
-
-
9
1
-
-
-
-
-
-
-
-
-
698266
Balogh-Hergovich
Copper-mediated oxygenation of ...
Cyberlindnera mrakii
Inorg. Chem.
37
6535-6537
1998
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697294
Balogh-Hergovich
-
Copper mediated conversion of ...
Cyberlindnera mrakii
Chem. Lett.
25
573-574
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639222
Kido
-
2-Nitropropane dioxygenase fro ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
2549-2554
1984
-
-
-
-
-
-
-
21
-
2
2
1
-
2
-
-
2
-
-
-
2
-
23
2
1
-
-
-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
21
-
2
2
1
-
-
-
2
-
-
2
-
23
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
639226
Kido
-
Characterization of primary ni ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
1361-1362
1984
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639227
Kido
Oxidation of anionic nitroalka ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
234
468-475
1984
-
-
-
-
-
-
11
2
-
1
-
-
-
9
-
-
1
1
-
-
-
-
8
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
2
-
1
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639224
Kido
Properties of 2-nitropropane d ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
253
226-232
1978
-
-
-
-
-
-
19
2
-
-
-
-
-
11
-
-
1
2
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
20
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639225
Kido
Purification and properties of ...
Fusarium oxysporum
J. Bacteriol.
133
53-58
1978
-
-
-
-
-
-
10
6
-
-
2
-
-
4
-
-
2
-
-
1
1
1
14
1
1
2
2
6
-
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
11
-
6
-
-
2
-
-
-
-
2
-
1
1
1
14
1
2
2
6
-
1
-
1
-
-
-
-
-
-
-
639223
Kido
A new oxygenase, 2-nitropropan ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
251
6994-7000
1976
3
-
-
-
-
3
13
7
-
1
4
-
-
8
-
-
1
-
-
-
-
-
17
1
1
1
1
-
-
2
2
-
2
-
-
-
3
-
-
2
-
-
3
-
18
-
7
-
1
4
-
-
-
-
1
-
-
-
-
17
1
1
1
-
-
2
2
-
-
-
-
-
-
-
-
639235
Kido
Purification and properties of ...
Cyberlindnera mrakii
J. Bacteriol.
126
1261-1265
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
1
1
-
5
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-