Reference to 1.13.12.16; Id = 674590 - BRENDA Enzyme Database

expressed in Escherichia coli C41(DE3) cells

Pseudomonas aeruginosa

hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000

Pseudomonas aeruginosa

H152A

His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon

Pseudomonas aeruginosa

H152A

site-directed mutagenesis. His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon

Pseudomonas aeruginosa

S288A

mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli

Pseudomonas aeruginosa

S288A

site-directed mutagenesis. Mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli

Pseudomonas aeruginosa

additional information

no inhibition by valeric acid and EDTA

Pseudomonas aeruginosa

37000

-

dynamic light scattering analysis

Pseudomonas aeruginosa

Q9I4V0

-

HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography

Pseudomonas aeruginosa

2-nitropropane + O2

-

674590

Pseudomonas aeruginosa

acetone + HNO2

-

?

2-nitropropane + O2

anionic form

674590

Pseudomonas aeruginosa

acetone + HNO2

-

?

monomer

1 * 37000, dynamic light scattering analysis

Pseudomonas aeruginosa

monomer

monomer 1 * 37000, dynamic light scattering analysis

Pseudomonas aeruginosa

2-nitropropane dioxygenase

-

Pseudomonas aeruginosa

30

-

assay at

Pseudomonas aeruginosa

6.5

-

assay at

Pseudomonas aeruginosa

FMN

-

Pseudomonas aeruginosa

expressed in Escherichia coli C41(DE3) cells

Pseudomonas aeruginosa

FMN

-

Pseudomonas aeruginosa

hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000

Pseudomonas aeruginosa

H152A

His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon

Pseudomonas aeruginosa

H152A

site-directed mutagenesis. His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon

Pseudomonas aeruginosa

S288A

mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli

Pseudomonas aeruginosa

S288A

site-directed mutagenesis. Mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli

Pseudomonas aeruginosa

additional information

no inhibition by valeric acid and EDTA

Pseudomonas aeruginosa

37000

-

dynamic light scattering analysis

Pseudomonas aeruginosa

HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography

Pseudomonas aeruginosa

2-nitropropane + O2

-

674590

Pseudomonas aeruginosa

acetone + HNO2

-

?

2-nitropropane + O2

anionic form

674590

Pseudomonas aeruginosa

acetone + HNO2

-

?

monomer

1 * 37000, dynamic light scattering analysis

Pseudomonas aeruginosa

monomer

monomer 1 * 37000, dynamic light scattering analysis

Pseudomonas aeruginosa

30

-

assay at

Pseudomonas aeruginosa

6.5

-

assay at

Pseudomonas aeruginosa

745219

Vercammen

Pseudomonas aeruginosa LysR P ...

Pseudomonas aeruginosa

J. Bacteriol.

197

1026-1039

2015

-

1

-

1

-

1

-

3

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

2

-

745306

Salvi

The combined structural and k ...

Pseudomonas aeruginosa

J. Biol. Chem.

289

23764-23775

2014

-

1

-

4

-

1

-

10

-

1

-

8

1

5

1

-

3

1

-

1

-

1

-

4

-

1

-

1

-

8

1

-

3

1

-

3

-

6

727018

Smitherman

Evidence for a transient perox ...

Cyberlindnera saturnus

Biochemistry

52

2694-2704

2013

-

6

-

3

-

2

-

1

2

-

5

1

-

6

-

2

-

2

-

5

1

-

6

728077

Klinkenberg

Rv1894c is a novel hypoxia-ind ...

Mycobacterium tuberculosis

J. Infect. Dis.

207

1525-1534

2013

-

1

-

1

-

1

-

10

-

1

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

726808

Francis

A novel activity for fungal ni ...

Cyberlindnera saturnus, Neurospora crassa

Arch. Biochem. Biophys.

521

84-89

2012

-

3

-

10

-

3

-

4

2

-

3

2

-

3

-

3

-

2

-

3

2

-

3

-

3

724175

Li

Crystal structure and site-dir ...

Streptomyces ansochromogenes

Biochem. Biophys. Res. Commun.

405

344-348

2011

-

1

3

-

2

-

3

-

2

-

1

-

1

-

1

3

-

3

-

1

-

695891

Gadda

Nitronate monooxygenase, a mod ...

Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii

Arch. Biochem. Biophys.

493

53-61

2009

-

1

-

3

-

13

-

6

-

3

-

16

1

3

-

3

-

3

-

3

-

1

-

3

-

13

-

16

1

3

-

3

-

696315

Francis

Inflated kinetic isotope effec ...

Neurospora crassa

Biochemistry

48

2403-2410

2009

-

6

-

1

-

1

-

684712

Mijatovic

Oxidation of alkyl nitronates ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

473

61-68

2008

-

1

-

1

-

1

-

6

-

1

-

2

-

8

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

8

-

1

-

2

-

6

685283

Francis

The nonoxidative conversion of ...

Neurospora crassa

Biochemistry

47

9136-9144

2008

-

1

-

2

-

1

6

-

4

-

1

-

5

-

1

-

2

1

-

2

-

1

-

2

-

1

2

6

-

1

-

5

-

1

-

2

1

-

4

672094

Francis

Probing the chemical steps of ...

Neurospora crassa

Biochemistry

45

13889-13898

2006

-

1

11

-

1

-

1

-

4

-

2

1

-

5

1

-

1

-

1

-

1

-

11

-

1

-

4

-

1

-

5

1

-

7

674590

Ha

Crystal structure of 2-nitropr ...

Pseudomonas aeruginosa

J. Biol. Chem.

281

18660-18667

2006

-

1

4

-

1

-

1

-

5

-

1

-

2

1

-

1

-

1

-

1

4

-

1

-

1

-

1

-

2

1

-

1

-

656289

Francis

Involvement of a flavosemiquin ...

Neurospora crassa

J. Biol. Chem.

280

5195-5204

2005

-

3

-

11

-

2

4

1

-

7

-

3

-

5

1

33

2

1

2

-

28

2

1

-

3

-

3

-

11

-

2

4

1

-

3

-

5

1

33

2

-

28

2

1

-

10

639233

Gadda

Substrate specificity of a nit ...

Fusarium oxysporum

Arch. Biochem. Biophys.

363

309-313

1999

-

1

-

1

-

3

-

14

-

1

-

2

-

1

2

-

1

-

2

-

1

-

14

-

1

-

2

-

639232

Gorlatova

Purification, characterization ...

Neurospora crassa

Appl. Environ. Microbiol.

64

1029-1033

1998

-

8

10

-

2

-

10

-

2

-

1

-

20

1

-

9

1

-

2

-

2

-

15

-

10

-

2

-

2

-

1

-

20

1

-

9

1

-

698266

Balogh-Hergovich

Copper-mediated oxygenation of ...

Cyberlindnera mrakii

Inorg. Chem.

37

6535-6537

1998

-

1

-

1

-

1

-

1

-

1

-

1

-

697294

Balogh-Hergovich

-

Copper mediated conversion of ...

Cyberlindnera mrakii

Chem. Lett.

25

573-574

1996

-

1

-

8

-

8

-

639222

Kido

-

2-Nitropropane dioxygenase fro ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

2549-2554

1984

-

21

-

2

1

-

2

-

2

-

2

-

23

2

1

-

4

-

2

-

2

-

21

-

2

1

-

2

-

2

-

23

2

-

4

-

639226

Kido

-

Characterization of primary ni ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

1361-1362

1984

-

1

-

1

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

4

-

639227

Kido

Oxidation of anionic nitroalka ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

234

468-475

1984

-

11

2

-

1

-

9

-

1

-

8

-

1

-

1

-

1

-

1

-

14

-

2

-

1

-

1

-

8

-

1

-

639224

Kido

Properties of 2-nitropropane d ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

253

226-232

1978

-

19

2

-

11

-

1

2

-

2

-

1

-

1

-

1

-

20

-

2

-

1

-

2

-

639225

Kido

Purification and properties of ...

Fusarium oxysporum

J. Bacteriol.

133

53-58

1978

-

10

6

-

2

-

4

-

2

-

1

14

1

2

6

-

1

-

1

3

-

3

-

11

-

6

-

2

-

2

-

1

14

1

2

6

-

1

-

1

-

639223

Kido

A new oxygenase, 2-nitropropan ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

251

6994-7000

1976

3

-

3

13

7

-

1

4

-

8

-

1

-

17

1

-

2

-

2

-

3

-

2

-

3

-

18

-

7

-

1

4

-

1

-

17

1

-

2

-

639235

Kido

Purification and properties of ...

Cyberlindnera mrakii

J. Bacteriol.

126

1261-1265

1976

-

5

-

1

-

1

-

5

1

-

2

-

1

-

1

-

5

1

-

2

-

Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base

Data extracted from this reference: