Data extracted from this reference:
Cloned(Commentary)
expressed in Escherichia coli C41(DE3) cells
Pseudomonas aeruginosa
Crystallization (Commentary)
hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000
Pseudomonas aeruginosa
Engineering
H152A
His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon
Pseudomonas aeruginosa
H152A
site-directed mutagenesis. His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon
Pseudomonas aeruginosa
S288A
mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli
Pseudomonas aeruginosa
S288A
site-directed mutagenesis. Mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli
Pseudomonas aeruginosa
Inhibitors
additional information
no inhibition by valeric acid and EDTA
Pseudomonas aeruginosa
Molecular Weight [Da]
37000
dynamic light scattering analysis
Pseudomonas aeruginosa
Organism
Pseudomonas aeruginosa
Q9I4V0
Purification (Commentary)
HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate)
2-nitropropane + O2
674590
Pseudomonas aeruginosa
acetone + HNO2
?
2-nitropropane + O2
anionic form
674590
Pseudomonas aeruginosa
acetone + HNO2
?
Subunits
monomer
1 * 37000, dynamic light scattering analysis
Pseudomonas aeruginosa
monomer
monomer 1 * 37000, dynamic light scattering analysis
Pseudomonas aeruginosa
Synonyms
2-nitropropane dioxygenase
Pseudomonas aeruginosa
Temperature Optimum [°C]
30
assay at
Pseudomonas aeruginosa
pH Optimum
6.5
assay at
Pseudomonas aeruginosa
Cofactor
FMN
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
expressed in Escherichia coli C41(DE3) cells
Pseudomonas aeruginosa
Cofactor (protein specific)
FMN
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000
Pseudomonas aeruginosa
Engineering (protein specific)
H152A
His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon
Pseudomonas aeruginosa
H152A
site-directed mutagenesis. His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon
Pseudomonas aeruginosa
S288A
mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli
Pseudomonas aeruginosa
S288A
site-directed mutagenesis. Mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli
Pseudomonas aeruginosa
Inhibitors (protein specific)
additional information
no inhibition by valeric acid and EDTA
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
37000
dynamic light scattering analysis
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
2-nitropropane + O2
674590
Pseudomonas aeruginosa
acetone + HNO2
?
2-nitropropane + O2
anionic form
674590
Pseudomonas aeruginosa
acetone + HNO2
?
Subunits (protein specific)
monomer
1 * 37000, dynamic light scattering analysis
Pseudomonas aeruginosa
monomer
monomer 1 * 37000, dynamic light scattering analysis
Pseudomonas aeruginosa
Temperature Optimum [°C] (protein specific)
30
assay at
Pseudomonas aeruginosa
pH Optimum (protein specific)
6.5
assay at
Pseudomonas aeruginosa
Other publictions for EC 1.13.12.16
745219
Vercammen
Pseudomonas aeruginosa LysR P ...
Pseudomonas aeruginosa
J. Bacteriol.
197
1026-1039
2015
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1
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1
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1
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1
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2
2
-
-
-
745306
Salvi
The combined structural and k ...
Pseudomonas aeruginosa
J. Biol. Chem.
289
23764-23775
2014
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-
1
1
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-
-
4
-
-
-
1
-
10
-
-
1
1
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8
1
5
1
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3
1
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1
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1
1
1
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1
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1
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8
1
1
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-
3
1
-
-
-
-
3
3
-
6
6
727018
Smitherman
Evidence for a transient perox ...
Cyberlindnera saturnus
Biochemistry
52
2694-2704
2013
-
-
-
-
-
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-
6
-
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3
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1
2
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1
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6
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2
-
2
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5
1
-
-
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-
-
-
-
6
6
728077
Klinkenberg
Rv1894c is a novel hypoxia-ind ...
Mycobacterium tuberculosis
J. Infect. Dis.
207
1525-1534
2013
-
-
1
-
1
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1
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-
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10
-
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1
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1
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1
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1
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1
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1
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1
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1
-
1
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-
-
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-
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1
-
-
1
-
-
726808
Francis
A novel activity for fungal ni ...
Cyberlindnera saturnus, Neurospora crassa
Arch. Biochem. Biophys.
521
84-89
2012
-
-
-
-
-
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3
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10
-
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2
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2
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3
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3
-
2
-
-
3
2
-
-
-
-
3
3
-
3
3
724175
Li
Crystal structure and site-dir ...
Streptomyces ansochromogenes
Biochem. Biophys. Res. Commun.
405
344-348
2011
-
-
1
1
3
-
-
-
-
-
-
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-
2
-
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3
-
2
-
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1
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-
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-
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-
1
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii
Arch. Biochem. Biophys.
493
53-61
2009
-
-
-
-
-
-
-
1
-
3
-
13
-
6
-
-
-
3
-
-
-
-
16
1
3
3
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
3
-
13
-
-
-
-
-
-
-
-
16
1
3
-
-
-
3
-
-
-
-
-
-
-
-
-
696315
Francis
Inflated kinetic isotope effec ...
Neurospora crassa
Biochemistry
48
2403-2410
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
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-
-
-
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-
-
-
-
-
-
-
-
684712
Mijatovic
Oxidation of alkyl nitronates ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
473
61-68
2008
-
-
1
-
-
-
1
-
-
1
1
-
-
6
-
-
1
-
-
2
-
-
8
-
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
-
2
-
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
6
6
685283
Francis
The nonoxidative conversion of ...
Neurospora crassa
Biochemistry
47
9136-9144
2008
-
-
1
-
2
-
1
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
5
-
1
1
-
-
2
1
1
-
-
2
-
-
-
-
1
-
-
2
-
-
1
2
6
-
-
-
-
-
-
-
1
-
-
-
-
5
-
1
-
-
2
1
1
-
-
-
-
-
-
4
4
672094
Francis
Probing the chemical steps of ...
Neurospora crassa
Biochemistry
45
13889-13898
2006
-
-
-
-
-
-
1
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
2
1
-
-
5
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
11
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
5
1
1
-
-
-
-
-
-
7
7
674590
Ha
Crystal structure of 2-nitropr ...
Pseudomonas aeruginosa
J. Biol. Chem.
281
18660-18667
2006
-
-
1
1
4
-
1
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
2
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
4
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656289
Francis
Involvement of a flavosemiquin ...
Neurospora crassa
J. Biol. Chem.
280
5195-5204
2005
-
-
3
-
-
-
-
11
-
2
4
1
-
7
-
-
3
-
-
-
5
1
33
2
1
2
-
-
28
2
1
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
11
-
2
4
1
-
-
-
3
-
-
5
1
33
2
2
-
-
28
2
1
-
-
-
-
-
-
10
10
639233
Gadda
Substrate specificity of a nit ...
Fusarium oxysporum
Arch. Biochem. Biophys.
363
309-313
1999
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
14
-
1
1
-
-
-
2
-
-
1
2
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
639232
Gorlatova
Purification, characterization ...
Neurospora crassa
Appl. Environ. Microbiol.
64
1029-1033
1998
-
-
-
-
-
-
8
10
-
-
2
-
-
10
-
-
2
-
-
1
1
-
20
1
1
1
-
-
9
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
15
-
10
-
-
2
-
-
-
-
2
-
1
1
-
20
1
1
-
-
9
1
-
-
-
-
-
-
-
-
-
698266
Balogh-Hergovich
Copper-mediated oxygenation of ...
Cyberlindnera mrakii
Inorg. Chem.
37
6535-6537
1998
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
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-
-
-
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-
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-
-
1
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697294
Balogh-Hergovich
-
Copper mediated conversion of ...
Cyberlindnera mrakii
Chem. Lett.
25
573-574
1996
-
-
-
-
-
-
-
-
-
-
-
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-
1
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8
-
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-
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-
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-
-
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-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639222
Kido
-
2-Nitropropane dioxygenase fro ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
2549-2554
1984
-
-
-
-
-
-
-
21
-
2
2
1
-
2
-
-
2
-
-
-
2
-
23
2
1
-
-
-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
21
-
2
2
1
-
-
-
2
-
-
2
-
23
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
639226
Kido
-
Characterization of primary ni ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
1361-1362
1984
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
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4
-
1
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-
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1
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1
-
-
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1
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1
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-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639227
Kido
Oxidation of anionic nitroalka ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
234
468-475
1984
-
-
-
-
-
-
11
2
-
1
-
-
-
9
-
-
1
1
-
-
-
-
8
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
2
-
1
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639224
Kido
Properties of 2-nitropropane d ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
253
226-232
1978
-
-
-
-
-
-
19
2
-
-
-
-
-
11
-
-
1
2
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
20
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639225
Kido
Purification and properties of ...
Fusarium oxysporum
J. Bacteriol.
133
53-58
1978
-
-
-
-
-
-
10
6
-
-
2
-
-
4
-
-
2
-
-
1
1
1
14
1
1
2
2
6
-
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
11
-
6
-
-
2
-
-
-
-
2
-
1
1
1
14
1
2
2
6
-
1
-
1
-
-
-
-
-
-
-
639223
Kido
A new oxygenase, 2-nitropropan ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
251
6994-7000
1976
3
-
-
-
-
3
13
7
-
1
4
-
-
8
-
-
1
-
-
-
-
-
17
1
1
1
1
-
-
2
2
-
2
-
-
-
3
-
-
2
-
-
3
-
18
-
7
-
1
4
-
-
-
-
1
-
-
-
-
17
1
1
1
-
-
2
2
-
-
-
-
-
-
-
-
639235
Kido
Purification and properties of ...
Cyberlindnera mrakii
J. Bacteriol.
126
1261-1265
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
1
1
-
5
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-