BRENDA - Reference to 1.13.12.16; Id = 684712

expressed in Escherichia coli

Cyberlindnera mrakii

additional information

superoxide dismutase and catalase have no effect on the enzymatic activity

Cyberlindnera mrakii

additional information

the enzyme contains negligible amounts of iron, manganese, zinc, and copper ions, which are not catalytically relevant

Cyberlindnera mrakii

40000

-

SDS-PAGE

Cyberlindnera mrakii

Q12723

-

Cyberlindnera mrakii

butyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + butanal

-

?

ethylnitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + acetaldehyde

-

?

hexyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + hexanal

-

?

additional information

anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicated that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes

684712

Cyberlindnera mrakii

?

-

?

additional information

anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicates that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes

684712

Cyberlindnera mrakii

?

-

?

pentyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + pentanal

-

?

propyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + propionaldehyde

-

?

propyl-2-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + acetone

-

?

2-nitropropane dioxygenase

-

Cyberlindnera mrakii

30

-

assay at

Cyberlindnera mrakii

7.4

-

assay at

Cyberlindnera mrakii

8

-

assay at

Cyberlindnera mrakii

FMN

present in a 1:1 stoichiometry with the protein. The tight binding of sulfite (Kd = 0.09 mM, at pH 8 and 15°C) to the enzyme and the formation of the anionic flavosemiquinone upon anaerobic incubation with alkyl nitronates are consistent with the presence of a positively charged group in proximity of the N(1)-C(2)=O atoms of the FMN cofactor

Cyberlindnera mrakii

expressed in Escherichia coli

Cyberlindnera mrakii

FMN

present in a 1:1 stoichiometry with the protein. The tight binding of sulfite (Kd = 0.09 mM, at pH 8 and 15°C) to the enzyme and the formation of the anionic flavosemiquinone upon anaerobic incubation with alkyl nitronates are consistent with the presence of a positively charged group in proximity of the N(1)-C(2)=O atoms of the FMN cofactor

Cyberlindnera mrakii

additional information

superoxide dismutase and catalase have no effect on the enzymatic activity

Cyberlindnera mrakii

additional information

the enzyme contains negligible amounts of iron, manganese, zinc, and copper ions, which are not catalytically relevant

Cyberlindnera mrakii

40000

-

SDS-PAGE

Cyberlindnera mrakii

-

Cyberlindnera mrakii

butyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + butanal

-

?

ethylnitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + acetaldehyde

-

?

hexyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + hexanal

-

?

additional information

anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicated that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes

684712

Cyberlindnera mrakii

?

-

?

additional information

anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicates that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes

684712

Cyberlindnera mrakii

?

-

?

pentyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + pentanal

-

?

propyl-1-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + propionaldehyde

-

?

propyl-2-nitronate + O2

-

684712

Cyberlindnera mrakii

NO2- + acetone

-

?

30

-

assay at

Cyberlindnera mrakii

7.4

-

assay at

Cyberlindnera mrakii

8

-

assay at

Cyberlindnera mrakii

1.6

-

propyl-2-nitronate

-

Cyberlindnera mrakii

112

-

butyl-1-nitronate

-

Cyberlindnera mrakii

121

-

pentyl-1-nitronate

-

Cyberlindnera mrakii

129

-

ethylnitronate

-

Cyberlindnera mrakii

130

-

hexyl-1-nitronate

-

Cyberlindnera mrakii

170

-

propyl-1-nitronate

-

Cyberlindnera mrakii

1.6

-

propyl-2-nitronate

-

Cyberlindnera mrakii

112

-

butyl-1-nitronate

-

Cyberlindnera mrakii

121

-

pentyl-1-nitronate

-

Cyberlindnera mrakii

129

-

ethylnitronate

-

Cyberlindnera mrakii

130

-

hexyl-1-nitronate

-

Cyberlindnera mrakii

170

-

propyl-1-nitronate

-

Cyberlindnera mrakii

745219

Vercammen

Pseudomonas aeruginosa LysR P ...

Pseudomonas aeruginosa

J. Bacteriol.

197

1026-1039

2015

-

1

-

1

-

1

-

3

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

2

-

745306

Salvi

The combined structural and k ...

Pseudomonas aeruginosa

J. Biol. Chem.

289

23764-23775

2014

-

1

-

4

-

1

-

10

-

1

-

8

1

5

1

-

3

1

-

1

-

1

-

4

-

1

-

1

-

8

1

-

3

1

-

3

-

6

727018

Smitherman

Evidence for a transient perox ...

Cyberlindnera saturnus

Biochemistry

52

2694-2704

2013

-

6

-

3

-

2

-

1

2

-

5

1

-

6

-

2

-

2

-

5

1

-

6

728077

Klinkenberg

Rv1894c is a novel hypoxia-ind ...

Mycobacterium tuberculosis

J. Infect. Dis.

207

1525-1534

2013

-

1

-

1

-

1

-

10

-

1

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

726808

Francis

A novel activity for fungal ni ...

Cyberlindnera saturnus, Neurospora crassa

Arch. Biochem. Biophys.

521

84-89

2012

-

3

-

10

-

3

-

4

2

-

3

2

-

3

-

3

-

2

-

3

2

-

3

-

3

724175

Li

Crystal structure and site-dir ...

Streptomyces ansochromogenes

Biochem. Biophys. Res. Commun.

405

344-348

2011

-

1

3

-

2

-

3

-

2

-

1

-

1

-

1

3

-

3

-

1

-

695891

Gadda

Nitronate monooxygenase, a mod ...

Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii

Arch. Biochem. Biophys.

493

53-61

2009

-

1

-

3

-

13

-

6

-

3

-

16

1

3

-

3

-

3

-

3

-

1

-

3

-

13

-

16

1

3

-

3

-

696315

Francis

Inflated kinetic isotope effec ...

Neurospora crassa

Biochemistry

48

2403-2410

2009

-

6

-

1

-

1

-

684712

Mijatovic

Oxidation of alkyl nitronates ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

473

61-68

2008

-

1

-

1

-

1

-

6

-

1

-

2

-

8

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

8

-

1

-

2

-

6

685283

Francis

The nonoxidative conversion of ...

Neurospora crassa

Biochemistry

47

9136-9144

2008

-

1

-

2

-

1

6

-

4

-

1

-

5

-

1

-

2

1

-

2

-

1

-

2

-

1

2

6

-

1

-

5

-

1

-

2

1

-

4

672094

Francis

Probing the chemical steps of ...

Neurospora crassa

Biochemistry

45

13889-13898

2006

-

1

11

-

1

-

1

-

4

-

2

1

-

5

1

-

1

-

1

-

1

-

11

-

1

-

4

-

1

-

5

1

-

7

674590

Ha

Crystal structure of 2-nitropr ...

Pseudomonas aeruginosa

J. Biol. Chem.

281

18660-18667

2006

-

1

4

-

1

-

1

-

5

-

1

-

2

1

-

1

-

1

-

1

4

-

1

-

1

-

1

-

2

1

-

1

-

656289

Francis

Involvement of a flavosemiquin ...

Neurospora crassa

J. Biol. Chem.

280

5195-5204

2005

-

3

-

11

-

2

4

1

-

7

-

3

-

5

1

33

2

1

2

-

28

2

1

-

3

-

3

-

11

-

2

4

1

-

3

-

5

1

33

2

-

28

2

1

-

10

639233

Gadda

Substrate specificity of a nit ...

Fusarium oxysporum

Arch. Biochem. Biophys.

363

309-313

1999

-

1

-

1

-

3

-

14

-

1

-

2

-

1

2

-

1

-

2

-

1

-

14

-

1

-

2

-

639232

Gorlatova

Purification, characterization ...

Neurospora crassa

Appl. Environ. Microbiol.

64

1029-1033

1998

-

8

10

-

2

-

10

-

2

-

1

-

20

1

-

9

1

-

2

-

2

-

15

-

10

-

2

-

2

-

1

-

20

1

-

9

1

-

698266

Balogh-Hergovich

Copper-mediated oxygenation of ...

Cyberlindnera mrakii

Inorg. Chem.

37

6535-6537

1998

-

1

-

1

-

1

-

1

-

1

-

1

-

697294

Balogh-Hergovich

-

Copper mediated conversion of ...

Cyberlindnera mrakii

Chem. Lett.

25

573-574

1996

-

1

-

8

-

8

-

639222

Kido

-

2-Nitropropane dioxygenase fro ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

2549-2554

1984

-

21

-

2

1

-

2

-

2

-

2

-

23

2

1

-

4

-

2

-

2

-

21

-

2

1

-

2

-

2

-

23

2

-

4

-

639226

Kido

-

Characterization of primary ni ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

1361-1362

1984

-

1

-

1

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

4

-

639227

Kido

Oxidation of anionic nitroalka ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

234

468-475

1984

-

11

2

-

1

-

9

-

1

-

8

-

1

-

1

-

1

-

1

-

14

-

2

-

1

-

1

-

8

-

1

-

639224

Kido

Properties of 2-nitropropane d ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

253

226-232

1978

-

19

2

-

11

-

1

2

-

2

-

1

-

1

-

1

-

20

-

2

-

1

-

2

-

639225

Kido

Purification and properties of ...

Fusarium oxysporum

J. Bacteriol.

133

53-58

1978

-

10

6

-

2

-

4

-

2

-

1

14

1

2

6

-

1

-

1

3

-

3

-

11

-

6

-

2

-

2

-

1

14

1

2

6

-

1

-

1

-

639223

Kido

A new oxygenase, 2-nitropropan ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

251

6994-7000

1976

3

-

3

13

7

-

1

4

-

8

-

1

-

17

1

-

2

-

2

-

3

-

2

-

3

-

18

-

7

-

1

4

-

1

-

17

1

-

2

-

639235

Kido

Purification and properties of ...

Cyberlindnera mrakii

J. Bacteriol.

126

1261-1265

1976

-

5

-

1

-

1

-

5

1

-

2

-

1

-

1

-

5

1

-

2

-

Oxidation of alkyl nitronates catalyzed by 2-nitropropane dioxygenase from Hansenula mrakii

Data extracted from this reference: