Data extracted from this reference:
Cloned(Commentary)
expressed in Escherichia coli
Cyberlindnera mrakii
Inhibitors
additional information
superoxide dismutase and catalase have no effect on the enzymatic activity
Cyberlindnera mrakii
Metals/Ions
additional information
the enzyme contains negligible amounts of iron, manganese, zinc, and copper ions, which are not catalytically relevant
Cyberlindnera mrakii
Molecular Weight [Da]
40000
SDS-PAGE
Cyberlindnera mrakii
Organism
Cyberlindnera mrakii
Q12723
Purification (Commentary)
Source Tissue
Substrates and Products (Substrate)
butyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + butanal
?
ethylnitronate + O2
684712
Cyberlindnera mrakii
NO2- + acetaldehyde
?
hexyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + hexanal
?
additional information
anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicated that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes
684712
Cyberlindnera mrakii
?
?
additional information
anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicates that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes
684712
Cyberlindnera mrakii
?
?
pentyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + pentanal
?
propyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + propionaldehyde
?
propyl-2-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + acetone
?
Synonyms
2-nitropropane dioxygenase
Cyberlindnera mrakii
Temperature Optimum [°C]
30
assay at
Cyberlindnera mrakii
pH Optimum
7.4
assay at
Cyberlindnera mrakii
8
assay at
Cyberlindnera mrakii
Cofactor
FMN
present in a 1:1 stoichiometry with the protein. The tight binding of sulfite (Kd = 0.09 mM, at pH 8 and 15°C) to the enzyme and the formation of the anionic flavosemiquinone upon anaerobic incubation with alkyl nitronates are consistent with the presence of a positively charged group in proximity of the N(1)-C(2)=O atoms of the FMN cofactor
Cyberlindnera mrakii
Cloned(Commentary) (protein specific)
expressed in Escherichia coli
Cyberlindnera mrakii
Cofactor (protein specific)
FMN
present in a 1:1 stoichiometry with the protein. The tight binding of sulfite (Kd = 0.09 mM, at pH 8 and 15°C) to the enzyme and the formation of the anionic flavosemiquinone upon anaerobic incubation with alkyl nitronates are consistent with the presence of a positively charged group in proximity of the N(1)-C(2)=O atoms of the FMN cofactor
Cyberlindnera mrakii
Inhibitors (protein specific)
additional information
superoxide dismutase and catalase have no effect on the enzymatic activity
Cyberlindnera mrakii
Metals/Ions (protein specific)
additional information
the enzyme contains negligible amounts of iron, manganese, zinc, and copper ions, which are not catalytically relevant
Cyberlindnera mrakii
Molecular Weight [Da] (protein specific)
40000
SDS-PAGE
Cyberlindnera mrakii
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
Substrates and Products (Substrate) (protein specific)
butyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + butanal
?
ethylnitronate + O2
684712
Cyberlindnera mrakii
NO2- + acetaldehyde
?
hexyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + hexanal
?
additional information
anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicated that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes
684712
Cyberlindnera mrakii
?
?
additional information
anaerobic substrate reduction and kinetic data using a Clark oxygen electrode to measure rates of oxygen consumption indicates that the enzyme is active on a broad range of alkyl nitronates, with a marked preference for unbranched substrates over propyl-2-nitronate. The enzyme utilizes alkyl nitronates for catalysis, but not nitroalkanes
684712
Cyberlindnera mrakii
?
?
pentyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + pentanal
?
propyl-1-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + propionaldehyde
?
propyl-2-nitronate + O2
684712
Cyberlindnera mrakii
NO2- + acetone
?
Temperature Optimum [°C] (protein specific)
30
assay at
Cyberlindnera mrakii
pH Optimum (protein specific)
7.4
assay at
Cyberlindnera mrakii
8
assay at
Cyberlindnera mrakii
kcat/KM [mM/s]
1.6
propyl-2-nitronate
Cyberlindnera mrakii
112
butyl-1-nitronate
Cyberlindnera mrakii
121
pentyl-1-nitronate
Cyberlindnera mrakii
129
ethylnitronate
Cyberlindnera mrakii
130
hexyl-1-nitronate
Cyberlindnera mrakii
170
propyl-1-nitronate
Cyberlindnera mrakii
kcat/KM [mM/s] (protein specific)
1.6
propyl-2-nitronate
Cyberlindnera mrakii
112
butyl-1-nitronate
Cyberlindnera mrakii
121
pentyl-1-nitronate
Cyberlindnera mrakii
129
ethylnitronate
Cyberlindnera mrakii
130
hexyl-1-nitronate
Cyberlindnera mrakii
170
propyl-1-nitronate
Cyberlindnera mrakii
Other publictions for EC 1.13.12.16
745219
Vercammen
Pseudomonas aeruginosa LysR P ...
Pseudomonas aeruginosa
J. Bacteriol.
197
1026-1039
2015
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1
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1
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1
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1
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2
2
-
-
-
745306
Salvi
The combined structural and k ...
Pseudomonas aeruginosa
J. Biol. Chem.
289
23764-23775
2014
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-
1
1
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-
4
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1
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10
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1
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1
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1
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8
1
1
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-
3
1
-
-
-
-
3
3
-
6
6
727018
Smitherman
Evidence for a transient perox ...
Cyberlindnera saturnus
Biochemistry
52
2694-2704
2013
-
-
-
-
-
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-
6
-
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3
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1
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6
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2
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2
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5
1
-
-
-
-
-
-
-
6
6
728077
Klinkenberg
Rv1894c is a novel hypoxia-ind ...
Mycobacterium tuberculosis
J. Infect. Dis.
207
1525-1534
2013
-
-
1
-
1
-
1
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10
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1
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1
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1
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1
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1
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-
-
-
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-
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1
-
-
1
-
-
726808
Francis
A novel activity for fungal ni ...
Cyberlindnera saturnus, Neurospora crassa
Arch. Biochem. Biophys.
521
84-89
2012
-
-
-
-
-
-
-
3
-
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10
-
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3
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4
2
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3
2
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3
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2
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3
2
-
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3
3
-
3
3
724175
Li
Crystal structure and site-dir ...
Streptomyces ansochromogenes
Biochem. Biophys. Res. Commun.
405
344-348
2011
-
-
1
1
3
-
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2
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3
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-
1
-
-
-
-
-
-
-
-
-
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii
Arch. Biochem. Biophys.
493
53-61
2009
-
-
-
-
-
-
-
1
-
3
-
13
-
6
-
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3
-
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16
1
3
3
-
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3
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3
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3
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1
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3
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13
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16
1
3
-
-
-
3
-
-
-
-
-
-
-
-
-
696315
Francis
Inflated kinetic isotope effec ...
Neurospora crassa
Biochemistry
48
2403-2410
2009
-
-
-
-
-
-
-
-
-
-
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-
6
-
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1
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1
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684712
Mijatovic
Oxidation of alkyl nitronates ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
473
61-68
2008
-
-
1
-
-
-
1
-
-
1
1
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6
-
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1
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2
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8
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1
1
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2
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1
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1
1
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1
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1
1
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1
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2
-
-
8
-
1
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-
-
2
-
-
-
-
-
-
-
6
6
685283
Francis
The nonoxidative conversion of ...
Neurospora crassa
Biochemistry
47
9136-9144
2008
-
-
1
-
2
-
1
6
-
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4
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1
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1
1
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1
1
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1
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1
2
6
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1
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5
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1
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2
1
1
-
-
-
-
-
-
4
4
672094
Francis
Probing the chemical steps of ...
Neurospora crassa
Biochemistry
45
13889-13898
2006
-
-
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1
11
-
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1
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1
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4
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2
1
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5
1
1
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1
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1
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1
-
11
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1
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-
4
-
1
-
-
5
1
1
-
-
-
-
-
-
7
7
674590
Ha
Crystal structure of 2-nitropr ...
Pseudomonas aeruginosa
J. Biol. Chem.
281
18660-18667
2006
-
-
1
1
4
-
1
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1
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5
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1
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2
2
1
1
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1
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1
1
1
4
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1
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1
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1
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2
2
1
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1
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-
-
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-
-
-
-
656289
Francis
Involvement of a flavosemiquin ...
Neurospora crassa
J. Biol. Chem.
280
5195-5204
2005
-
-
3
-
-
-
-
11
-
2
4
1
-
7
-
-
3
-
-
-
5
1
33
2
1
2
-
-
28
2
1
-
3
-
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-
3
3
-
-
-
-
-
-
11
-
2
4
1
-
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3
-
-
5
1
33
2
2
-
-
28
2
1
-
-
-
-
-
-
10
10
639233
Gadda
Substrate specificity of a nit ...
Fusarium oxysporum
Arch. Biochem. Biophys.
363
309-313
1999
-
-
-
-
-
-
1
-
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1
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3
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14
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1
1
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2
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1
2
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1
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2
2
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1
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14
-
1
-
-
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2
-
-
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-
-
-
-
-
-
639232
Gorlatova
Purification, characterization ...
Neurospora crassa
Appl. Environ. Microbiol.
64
1029-1033
1998
-
-
-
-
-
-
8
10
-
-
2
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10
-
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2
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1
1
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20
1
1
1
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9
1
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2
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-
-
-
-
-
2
-
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-
-
15
-
10
-
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2
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2
-
1
1
-
20
1
1
-
-
9
1
-
-
-
-
-
-
-
-
-
698266
Balogh-Hergovich
Copper-mediated oxygenation of ...
Cyberlindnera mrakii
Inorg. Chem.
37
6535-6537
1998
-
-
-
1
-
-
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-
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1
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1
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1
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1
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1
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-
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-
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-
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-
-
-
-
697294
Balogh-Hergovich
-
Copper mediated conversion of ...
Cyberlindnera mrakii
Chem. Lett.
25
573-574
1996
-
-
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-
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1
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8
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8
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-
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-
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-
-
-
-
-
-
-
-
639222
Kido
-
2-Nitropropane dioxygenase fro ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
2549-2554
1984
-
-
-
-
-
-
-
21
-
2
2
1
-
2
-
-
2
-
-
-
2
-
23
2
1
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4
-
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2
-
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-
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-
2
-
-
-
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-
-
21
-
2
2
1
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-
2
-
-
2
-
23
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
639226
Kido
-
Characterization of primary ni ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
1361-1362
1984
-
-
-
-
-
-
-
1
-
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1
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1
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4
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1
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1
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1
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1
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1
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-
4
-
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-
-
-
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-
-
-
-
-
-
-
-
639227
Kido
Oxidation of anionic nitroalka ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
234
468-475
1984
-
-
-
-
-
-
11
2
-
1
-
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-
9
-
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1
1
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8
-
1
-
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-
-
1
-
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1
-
-
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-
1
-
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-
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14
-
2
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1
-
-
-
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1
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-
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8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639224
Kido
Properties of 2-nitropropane d ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
253
226-232
1978
-
-
-
-
-
-
19
2
-
-
-
-
-
11
-
-
1
2
-
-
-
-
2
-
1
-
-
-
-
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-
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1
-
-
-
-
-
-
1
-
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20
-
2
-
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-
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1
-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639225
Kido
Purification and properties of ...
Fusarium oxysporum
J. Bacteriol.
133
53-58
1978
-
-
-
-
-
-
10
6
-
-
2
-
-
4
-
-
2
-
-
1
1
1
14
1
1
2
2
6
-
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
11
-
6
-
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2
-
-
-
-
2
-
1
1
1
14
1
2
2
6
-
1
-
1
-
-
-
-
-
-
-
639223
Kido
A new oxygenase, 2-nitropropan ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
251
6994-7000
1976
3
-
-
-
-
3
13
7
-
1
4
-
-
8
-
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1
-
-
-
-
-
17
1
1
1
1
-
-
2
2
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2
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3
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2
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3
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18
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7
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1
4
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1
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17
1
1
1
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2
2
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639235
Kido
Purification and properties of ...
Cyberlindnera mrakii
J. Bacteriol.
126
1261-1265
1976
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