BRENDA - Enzyme Database
show all sequences of 1.13.12.16

Evidence for a transient peroxynitro acid in the reaction catalyzed by nitronate monooxygenase with propionate 3-nitronate

Smitherman, C.; Gadda, G.; Biochemistry 52, 2694-2704 (2013)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
propionate 3-nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
0.08
-
O2
pH 5.5, 30°C
Cyberlindnera saturnus
4
-
ethyl nitronate
pH 8, 30°C
Cyberlindnera saturnus
4
-
ethyl nitronate
pH 6, 30°C
Cyberlindnera saturnus
10
-
ethyl nitronate
pH 11, 30°C
Cyberlindnera saturnus
20
-
ethyl nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
Organism
Organism
UniProt
Commentary
Textmining
Cyberlindnera saturnus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ethyl nitronate + O2
-
727018
Cyberlindnera saturnus
? + nitrite
-
-
-
?
propionate 3-nitronate + O2
-
727018
Cyberlindnera saturnus
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
nitronate monooxygenase
-
Cyberlindnera saturnus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7
-
assay at
Cyberlindnera saturnus
30
-
assay at
Cyberlindnera saturnus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
40
-
ethyl nitronate
pH 6, 30°C
Cyberlindnera saturnus
40
-
ethyl nitronate
pH 11, 30°C
Cyberlindnera saturnus
840
-
ethyl nitronate
pH 8, 30°C
Cyberlindnera saturnus
860
-
propionate 3-nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
4100
-
ethyl nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Cyberlindnera saturnus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
propionate 3-nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
0.08
-
O2
pH 5.5, 30°C
Cyberlindnera saturnus
4
-
ethyl nitronate
pH 8, 30°C
Cyberlindnera saturnus
4
-
ethyl nitronate
pH 6, 30°C
Cyberlindnera saturnus
10
-
ethyl nitronate
pH 11, 30°C
Cyberlindnera saturnus
20
-
ethyl nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ethyl nitronate + O2
-
727018
Cyberlindnera saturnus
? + nitrite
-
-
-
?
propionate 3-nitronate + O2
-
727018
Cyberlindnera saturnus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7
-
assay at
Cyberlindnera saturnus
30
-
assay at
Cyberlindnera saturnus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
40
-
ethyl nitronate
pH 6, 30°C
Cyberlindnera saturnus
40
-
ethyl nitronate
pH 11, 30°C
Cyberlindnera saturnus
840
-
ethyl nitronate
pH 8, 30°C
Cyberlindnera saturnus
860
-
propionate 3-nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
4100
-
ethyl nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Cyberlindnera saturnus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
41
-
ethyl nitronate
pH 11, 30°C
Cyberlindnera saturnus
46
-
ethyl nitronate
pH 6, 30°C
Cyberlindnera saturnus
200
-
ethyl nitronate
pH 8, 30°C
Cyberlindnera saturnus
300
-
ethyl nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
11000
-
O2
pH 5.5, 30°C
Cyberlindnera saturnus
14330
-
propionate 3-nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
41
-
ethyl nitronate
pH 11, 30°C
Cyberlindnera saturnus
46
-
ethyl nitronate
pH 6, 30°C
Cyberlindnera saturnus
200
-
ethyl nitronate
pH 8, 30°C
Cyberlindnera saturnus
300
-
ethyl nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
11000
-
O2
pH 5.5, 30°C
Cyberlindnera saturnus
14330
-
propionate 3-nitronate
pH 5.5, 30°C
Cyberlindnera saturnus
Other publictions for EC 1.13.12.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745219
Vercammen
Pseudomonas aeruginosa LysR P ...
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1
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5
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
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1
-
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-
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Salvi
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Pseudomonas aeruginosa
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-
8
1
5
1
-
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3
1
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1
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1
1
1
-
-
-
-
-
4
-
-
-
1
-
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1
-
-
-
-
8
1
1
-
-
3
1
-
-
-
-
3
3
-
6
6
727018
Smitherman
Evidence for a transient perox ...
Cyberlindnera saturnus
Biochemistry
52
2694-2704
2013
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-
-
-
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-
-
6
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3
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2
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1
2
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5
1
-
-
-
-
-
-
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-
-
-
-
-
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-
-
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6
-
-
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-
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-
-
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2
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2
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5
1
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728077
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1
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1
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1
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-
-
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1
-
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1
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1
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3
-
4
2
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
3
2
-
-
-
-
3
3
-
3
3
724175
Li
Crystal structure and site-dir ...
Streptomyces ansochromogenes
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344-348
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-
-
1
1
3
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2
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-
3
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2
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1
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-
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-
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-
1
-
1
3
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
695891
Gadda
Nitronate monooxygenase, a mod ...
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2009
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-
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-
-
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1
-
3
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13
-
6
-
-
-
3
-
-
-
-
16
1
3
3
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
3
-
13
-
-
-
-
-
-
-
-
16
1
3
-
-
-
3
-
-
-
-
-
-
-
-
-
696315
Francis
Inflated kinetic isotope effec ...
Neurospora crassa
Biochemistry
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2403-2410
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-
-
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6
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
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-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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Oxidation of alkyl nitronates ...
Cyberlindnera mrakii
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61-68
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1
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1
-
-
6
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-
1
-
-
2
-
-
8
-
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
-
2
-
-
8
-
1
-
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2
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-
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6
6
685283
Francis
The nonoxidative conversion of ...
Neurospora crassa
Biochemistry
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1
-
2
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1
6
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-
-
-
4
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-
1
-
-
-
-
-
5
-
1
1
-
-
2
1
1
-
-
2
-
-
-
-
1
-
-
2
-
-
1
2
6
-
-
-
-
-
-
-
1
-
-
-
-
5
-
1
-
-
2
1
1
-
-
-
-
-
-
4
4
672094
Francis
Probing the chemical steps of ...
Neurospora crassa
Biochemistry
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-
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1
11
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1
-
-
1
-
-
-
-
-
4
-
2
1
-
-
5
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
11
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
5
1
1
-
-
-
-
-
-
7
7
674590
Ha
Crystal structure of 2-nitropr ...
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-
-
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4
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-
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1
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-
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-
-
2
2
1
1
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-
1
-
-
1
-
-
-
-
-
1
1
1
4
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
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2
2
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656289
Francis
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-
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3
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11
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4
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3
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-
-
5
1
33
2
1
2
-
-
28
2
1
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
11
-
2
4
1
-
-
-
3
-
-
5
1
33
2
2
-
-
28
2
1
-
-
-
-
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639233
Gadda
Substrate specificity of a nit ...
Fusarium oxysporum
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-
-
-
-
-
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1
-
-
-
-
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-
3
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-
-
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-
-
-
-
14
-
1
1
-
-
-
2
-
-
1
2
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
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Gorlatova
Purification, characterization ...
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-
-
-
-
-
-
8
10
-
-
2
-
-
10
-
-
2
-
-
1
1
-
20
1
1
1
-
-
9
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
15
-
10
-
-
2
-
-
-
-
2
-
1
1
-
20
1
1
-
-
9
1
-
-
-
-
-
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698266
Balogh-Hergovich
Copper-mediated oxygenation of ...
Cyberlindnera mrakii
Inorg. Chem.
37
6535-6537
1998
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
1
-
1
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-
-
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-
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-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697294
Balogh-Hergovich
-
Copper mediated conversion of ...
Cyberlindnera mrakii
Chem. Lett.
25
573-574
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639222
Kido
-
2-Nitropropane dioxygenase fro ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
2549-2554
1984
-
-
-
-
-
-
-
21
-
2
2
1
-
2
-
-
2
-
-
-
2
-
23
2
1
-
-
-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
21
-
2
2
1
-
-
-
2
-
-
2
-
23
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
639226
Kido
-
Characterization of primary ni ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
1361-1362
1984
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
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-
1
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1
-
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-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639227
Kido
Oxidation of anionic nitroalka ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
234
468-475
1984
-
-
-
-
-
-
11
2
-
1
-
-
-
9
-
-
1
1
-
-
-
-
8
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
2
-
1
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639224
Kido
Properties of 2-nitropropane d ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
253
226-232
1978
-
-
-
-
-
-
19
2
-
-
-
-
-
11
-
-
1
2
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
20
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639225
Kido
Purification and properties of ...
Fusarium oxysporum
J. Bacteriol.
133
53-58
1978
-
-
-
-
-
-
10
6
-
-
2
-
-
4
-
-
2
-
-
1
1
1
14
1
1
2
2
6
-
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
11
-
6
-
-
2
-
-
-
-
2
-
1
1
1
14
1
2
2
6
-
1
-
1
-
-
-
-
-
-
-
639223
Kido
A new oxygenase, 2-nitropropan ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
251
6994-7000
1976
3
-
-
-
-
3
13
7
-
1
4
-
-
8
-
-
1
-
-
-
-
-
17
1
1
1
1
-
-
2
2
-
2
-
-
-
3
-
-
2
-
-
3
-
18
-
7
-
1
4
-
-
-
-
1
-
-
-
-
17
1
1
1
-
-
2
2
-
-
-
-
-
-
-
-
639235
Kido
Purification and properties of ...
Cyberlindnera mrakii
J. Bacteriol.
126
1261-1265
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
1
1
-
5
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-