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Literature summary for 1.13.12.16 extracted from

  • Vercammen, K.; Wei, Q.; Charlier, D.; Doetsch, A.; Hauessler, S.; Schulz, S.; Salvi, F.; Gadda, G.; Spain, J.; Rybtke, M.L.; Tolker-Nielsen, T.; Dingemans, J.; Ye, L.; Cornelis, P.
    Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase (2015), J. Bacteriol., 197, 1026-1039 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PA4202 or nmoA, monocistronic, low expression level gene, has an intergenic region of 97 nucleotides with neighbouring gene PA4201 (ddlA) encoding D-alanine alanine ligase, and an intergenic region of 107 nucleotides with gene PA4203 encoding a LysR regulator, PA4203 (nmoR) represses its own transcription and the expression of PA4202 (nmoA), presence of a single NmoR binding site between nmoA and nmoR, and interaction of NmoR with the intergenic nmoA-nmoR control region. In silico analysis of the ddlA-nmoA-nmoR-ppgL locus in Pseudomonas aeruginosa genomes, transcriptome analysis Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme deletion mutant by the in vivo recombination system of the yeast Saccharomyces cerevisiae strain InvSc1, overview. Quantitative RT-PCR enzyme expression analysis. The expression of ddlA is not changed in the DELTAnmoA mutant compared to the wild-type Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 propionate-3-nitronate + O2 Pseudomonas aeruginosa
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3 malonic semialdehyde + nitrite + 2 nitrate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HWH9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 propionate-3-nitronate + O2
-
Pseudomonas aeruginosa 3 malonic semialdehyde + nitrite + 2 nitrate + H2O2
-
?

Synonyms

Synonyms Comment Organism
nitropropane dioxygenase
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Pseudomonas aeruginosa
nmoA
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Pseudomonas aeruginosa
P3N monooxygenase
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Pseudomonas aeruginosa
PA4202
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Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FMN a flavoprotein Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction deletion of the nmoA gene results in an increased sensitivity of the cells to 3-nitropropionic acid (3-NPA), phenotype, overview Pseudomonas aeruginosa
physiological function role of the nitronate monooxygenase protein in the detoxification of nitronate. At physiological pH, 3-nitropropionate, a nitro toxin produced by plants and fungi, exists in equilibrium with its conjugate base, propionate-3-nitronate, the tauromer of 3-nitropropionic acid Pseudomonas aeruginosa