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Literature summary for extracted from

  • Fukuda, H.; Ogawa, T.; Tazaki, M.; Nagahama, K.; Fujii, T.; Tanase, S.; Morino, Y.
    Two reactions are simultaneously catalyzed by a single enzyme: The arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae (1992), Biochem. Biophys. Res. Commun., 188, 483-489.
    View publication on PubMed


Metals/Ions Comment Organism Structure
Fe2+ at the active site, enzyme acts as a bidentate ligand and it forms a complex with Fe2+. The Fe2+ is further coordinated to a tridentate Schiff base of 2-oxoglutarate and L-arginine, whose terminal carboxylate and guanidino groups are trapped by binding sites I and II on the enzyme, respectively Pseudomonas syringae


Organism UniProt Comment Textmining
Pseudomonas syringae P32021 pv. phaseolicola PK2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + O2 presence of oxygen is essential for the ethylene forming reaction by EFE Pseudomonas syringae ethylene + ?
additional information enzyme catalyzes the formation of ethylene and succinate from 2-oxoglutarate, at a molar ratio of 2:l, reactions of EC and EC In the main reaction, 2-oxoglutarate is dioxygenated to produce one molecule of ethylene and three molecules of carbon dioxide. In the sub-reaction, both 2-oxoglutarate and L-arginine are mono-oxygenated to yield succinate plus carbon dioxide and L-hydroxyarginine, respectively, the latter being further transformed to guanidine and L-delta-pyrroline-5-carboxylate. Dual-circuit mechanism for the entire reaction is proposed, in which the binding of L-arginine and 2-oxoglutarate in a Schiff-base structure generates a common intermediate for two reactions Pseudomonas syringae ?


Synonyms Comment Organism
Pseudomonas syringae
ethylene-forming enzyme
Pseudomonas syringae