Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.12.19 extracted from

  • Nagahama, K.; Ogawa, T.; Fujii, T.; Tazaki, M.; Tanase, S.; Morino, Y.; Fukuda, H.
    Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. Phaseolicola PK2 (1991), J. Gen. Microbiol., 137, 2281-2286.
    View publication on PubMed
Search for more literature for 1.13.12.19

Activating Compound

Activating Compound Comment Organism Structure
D-arginine 3% of the activity with L-arginine Pseudomonas syringae
L-arginine highly specific for cofactor L-arginine, KM value 0.018 mM Pseudomonas syringae
L-canavanine sulfate 7% of the activity with L-arginine Pseudomonas syringae

Inhibitors

Inhibitors Comment Organism Structure
4,5-dihydroxy-1,3-benzene disulfonic acid 1 mM, 0.8% residual activity Pseudomonas syringae
5,5'-dithio-bis(2-nitrobenzoate) 1 mM, 0.7% residual activity Pseudomonas syringae
CoCl2 1 mM, 20% residual activity Pseudomonas syringae
CuSO4 1 mM, 50% residual activity Pseudomonas syringae
EDTA 1 mM, 1% residual activity Pseudomonas syringae
H2O2 1 mM, 0.7% residual activity Pseudomonas syringae
MnCl2 1 mM, 6% residual activity Pseudomonas syringae
n-propyl gallate 1 mM, 1% residual activity Pseudomonas syringae
Sodium azide 1 mM, 90% residual activity Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 2-oxoglutarate pH 8.0, 25°C Pseudomonas syringae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, KM value 0.059 mM Pseudomonas syringae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel fitlration Pseudomonas syringae
42000
-
 1 * 42000, SDS-PAGE Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae P32021 pv. phaseolicola PK2, enzyme catalyzes the formation of ethylene and succinate from 2-oxoglutarate, reactions of EC 1.13.12.19 and EC 1.14.11.34
-

Purification (Commentary)

Purification (Comment) Organism
from cell-free extract Pseudomonas syringae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
660
-
 pH 8.0, 25°C Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + O2 enzyme is highly specific for substrate 2-oxoglutarate Pseudomonas syringae ethylene + 3 CO2 + H2O
-
 ?
additional information presence of 2-oxoglutarate, L-arginine, Fe2+ and oxygen is essential for the enzymic reaction Pseudomonas syringae ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25
-
 Pseudomonas syringae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below Pseudomonas syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Pseudomonas syringae

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas syringae isoelectric focusing
-
 5.9