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Literature summary for 1.13.12.2 extracted from
- Purification and properties of L-lysine monooxygenase from Pseudomonas fluorescens (1974), J. Biol. Chem., 249, 2579-2586.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | at low lysine concentrations, inhibition by a variety of buffers | Pseudomonas fluorescens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
4 * 61000, SDS-PAGE | Pseudomonas fluorescens |
| 246000 | - |
gel filtration | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + O2 | monooxygenase activity | Pseudomonas fluorescens | 5-aminopentanamide + CO2 + H2O | - |
? |  |
| additional information | enzyme specific for O2 as electron acceptor, no dehydrogenase activity | Pseudomonas fluorescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 61000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | at low L-lysine concentration | Pseudomonas fluorescens |
| 9.5 | - |
at saturating L-lysine concentration, 37 mM lysine | Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | 9.4 | at pH 8.4 about 50% of activity maximum, at pH 9.4 about 40% of activity maximum | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 4 FAD prosthetic groups per enzyme | Pseudomonas fluorescens | |
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