Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not stimulated by addition of 2-oxoglutarate | Streptomyces roseochromogenus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces roseochromogenus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity | Streptomyces roseochromogenus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dimethylallyl-4-hydroxymandelate + O2 | Streptomyces roseochromogenus | - |
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? | |
3-dimethylallyl-4-hydroxymandelate + O2 | Streptomyces roseochromogenus DS 12976 | - |
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces roseochromogenus | Q8GHB1 | - |
- |
Streptomyces roseochromogenus DS 12976 | Q8GHB1 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Streptomyces roseochromogenus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dimethylallyl-4-hydroxymandelate + O2 | - |
Streptomyces roseochromogenus | 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? | |
3-dimethylallyl-4-hydroxymandelate + O2 | - |
Streptomyces roseochromogenus DS 12976 | 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30500, calculated from amino acid sequence | Streptomyces roseochromogenus |
Synonyms | Comment | Organism |
---|---|---|
CloR | - |
Streptomyces roseochromogenus |