Application | Comment | Organism |
---|---|---|
synthesis | overexpression of apoaequorin in Escherichia coli, solubilization of inclusion bodies with urea, purification and refolding of His6-apoaequorin in a single chromatographic step by immobilized metal-ion affinity chromatography using Ni2+-nitrilotriacetic acid agarose.The purity is greater than 80%, the yield is 0.71mg apoaequorin from a 50 ml bacterial culture. The luminescence of the purified aequorin is a linear function of its concentration extending over six orders of magnitude | Aequorea victoria |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aequorea victoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aequorea victoria | P02592 | - |
- |