Literature summary for 1.13.12.24 extracted from

Zeinoddini, M.; Fathi-Roudsari, M.; Hosseinkhani, S.; Khajeh, K.
Bioluminescence and kinetic aspects of double mutated aequorin variants (2018), Int. J. Biol. Macromol., 112, 163-168 .

Search for more literature for 1.13.12.24

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL-21(DE3)	Aequorea victoria

Protein Variants

Protein Variants	Comment	Organism
D153G	shift of maximum luminescence to shorter wavelengths. t1/2 is decreased with respect to the half-life time of the native protein (0.7 s). Mutation results in a significant decrease in stability at 65°C	Aequorea victoria
W86F	shift of maximum luminescence to shorter wavelengths. The single mutant reaches the highest stability against thermal shock	Aequorea victoria
W86F/D153G	decreased t1/2-value. Maximum emission spectrum at 401 nm. Mutation results in a significant decrease in stability at 65°C	Aequorea victoria
Y82F	mutation shifts the peak towards longer wavelengths. Increased half time of initial light by 3.6 s. Mutation results in a significant decrease in stability at 65°C	Aequorea victoria
Y82F/D153G	decreased t1/2-value. Maximum emission spectrum at 478 nm	Aequorea victoria
Y82F/W86F	maximum emission spectrum at 400 nm. Y82F mutation results in shift of emission to longer wavelength, while the W86F mutation shifts the emission to shorter wavelengths. Compared to wild type aequorin, the Y82F/W86F variant displays a 2fold increase of light half-life. Mutation results in a significant decrease in stability at 65°C	Aequorea victoria

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	calcium-regulated photoprotein	Aequorea victoria

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	SDS-PAGE	Aequorea victoria

Organism

Organism	UniProt	Comment	Textmining
Aequorea victoria	P02592	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Aequorea victoria

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[apoaequorin] + coelenterazine + O2 + 3 Ca2+	flash type light emission	Aequorea victoria	[excited state blue fluorescent protein] + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 26000, SDS-PAGE	Aequorea victoria

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Release 2023.1 (January 2023)