BRENDA - Enzyme Database show
show all sequences of 1.13.12.3

Microbial synthesis and degradation of indole-3-acetic acid. I. The conversion of L-tryptophan to indole-3-acetamide by an enzyme system from Pseudomonas savastanoi

Kosuge, T.; Heskett, M.G.; Wilson, E.E.; J. Biol. Chem. 241, 3738-3744 (1966)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
weak
Pseudomonas savastanoi
BaCl2
weak
Pseudomonas savastanoi
CaCl2
little or no effect
Pseudomonas savastanoi
CuCl2
-
Pseudomonas savastanoi
D-tryptophan
weak
Pseudomonas savastanoi
EDTA
little or no effect
Pseudomonas savastanoi
HgCl2
-
Pseudomonas savastanoi
hydroxylamine
-
Pseudomonas savastanoi
Indole-3-acetaldehyde
weak
Pseudomonas savastanoi
Indole-3-acetamide
end product inhibition
Pseudomonas savastanoi
indole-3-acetic acid
-
Pseudomonas savastanoi
iodoacetamide
-
Pseudomonas savastanoi
isoniazid
-
Pseudomonas savastanoi
K3Fe(CN)6
weak
Pseudomonas savastanoi
KCN
little or no effect
Pseudomonas savastanoi
L-cysteine
-
Pseudomonas savastanoi
L-glutathione
reduced
Pseudomonas savastanoi
Quinacrine
weak
Pseudomonas savastanoi
Semicarbazide
-
Pseudomonas savastanoi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Pseudomonas savastanoi
specific for L-isomer of tryptophan
indole-3-acetamide + CO2 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas savastanoi
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas savastanoi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.25
-
-
Pseudomonas savastanoi
Storage Stability
Storage Stability
Organism
-10°C, protein concentration 4 mg/ml, 2 months, less than 10% loss of activity
Pseudomonas savastanoi
-10°C, protein concentration less than 0.3 mg/ml, 40% loss of activity after 24 h
Pseudomonas savastanoi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxytryptophan + O2
at 17% the rate of that for L-tryptophan
439341
Pseudomonas savastanoi
5-hydroxyindole-3-acetamide + CO2 + H2O
-
-
-
?
L-phenylalanine + O2
weak activity
439341
Pseudomonas savastanoi
phenylacetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
specific for L-isomer of tryptophan
439341
Pseudomonas savastanoi
indole-3-acetamide + CO2 + H2O
-
-
-
?
L-tyrosine + O2
weak activity
439341
Pseudomonas savastanoi
4-hydroxyphenylacetamide + CO2 + H2O
-
-
-
?
additional information
no activity with: indole-3-pyruvate, indole-3-acetaldehyde, alpha-methyltryptophan, indole-3-acetic acid
439341
Pseudomonas savastanoi
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas savastanoi
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
pH 7.4, protein concentration above 4 mg/ml, 2 min, stable, 40% loss of activity after 5 min
Pseudomonas savastanoi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
9.2
-
Pseudomonas savastanoi
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
pH 5.5: about 50% of activity maximum, pH 10: about 55% of activity maximum
Pseudomonas savastanoi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
weak
Pseudomonas savastanoi
BaCl2
weak
Pseudomonas savastanoi
CaCl2
little or no effect
Pseudomonas savastanoi
CuCl2
-
Pseudomonas savastanoi
D-tryptophan
weak
Pseudomonas savastanoi
EDTA
little or no effect
Pseudomonas savastanoi
HgCl2
-
Pseudomonas savastanoi
hydroxylamine
-
Pseudomonas savastanoi
Indole-3-acetaldehyde
weak
Pseudomonas savastanoi
Indole-3-acetamide
end product inhibition
Pseudomonas savastanoi
indole-3-acetic acid
-
Pseudomonas savastanoi
iodoacetamide
-
Pseudomonas savastanoi
isoniazid
-
Pseudomonas savastanoi
K3Fe(CN)6
weak
Pseudomonas savastanoi
KCN
little or no effect
Pseudomonas savastanoi
L-cysteine
-
Pseudomonas savastanoi
L-glutathione
reduced
Pseudomonas savastanoi
Quinacrine
weak
Pseudomonas savastanoi
Semicarbazide
-
Pseudomonas savastanoi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Pseudomonas savastanoi
specific for L-isomer of tryptophan
indole-3-acetamide + CO2 + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas savastanoi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.25
-
-
Pseudomonas savastanoi
Storage Stability (protein specific)
Storage Stability
Organism
-10°C, protein concentration 4 mg/ml, 2 months, less than 10% loss of activity
Pseudomonas savastanoi
-10°C, protein concentration less than 0.3 mg/ml, 40% loss of activity after 24 h
Pseudomonas savastanoi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxytryptophan + O2
at 17% the rate of that for L-tryptophan
439341
Pseudomonas savastanoi
5-hydroxyindole-3-acetamide + CO2 + H2O
-
-
-
?
L-phenylalanine + O2
weak activity
439341
Pseudomonas savastanoi
phenylacetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
specific for L-isomer of tryptophan
439341
Pseudomonas savastanoi
indole-3-acetamide + CO2 + H2O
-
-
-
?
L-tyrosine + O2
weak activity
439341
Pseudomonas savastanoi
4-hydroxyphenylacetamide + CO2 + H2O
-
-
-
?
additional information
no activity with: indole-3-pyruvate, indole-3-acetaldehyde, alpha-methyltryptophan, indole-3-acetic acid
439341
Pseudomonas savastanoi
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas savastanoi
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
pH 7.4, protein concentration above 4 mg/ml, 2 min, stable, 40% loss of activity after 5 min
Pseudomonas savastanoi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
9.2
-
Pseudomonas savastanoi
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
pH 5.5: about 50% of activity maximum, pH 10: about 55% of activity maximum
Pseudomonas savastanoi
Other publictions for EC 1.13.12.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746273
Zhang
Regulation of oncogene expres ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
PLoS Pathog.
11
1-27
2015
-
-
1
-
-
-
-
-
-
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2
-
2
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2
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2
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2
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
745813
Yin
Characterization of a tryptop ...
Puccinia graminis f. sp. tritici, Puccinia graminis f. sp. tritici CRL 75-36-700-3
Mol. Plant Microbe Interact.
27
227-235
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
746257
Mujahid
Aniline is an inducer, and no ...
Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2
PLoS ONE
9
e87503
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
727017
Gaweska
Structure of the flavoprotein ...
Pseudomonas savastanoi
Biochemistry
52
2620-2626
2013
-
-
1
1
3
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
5
5
726752
Mujahid
Production of indole-3-acetic ...
Rubrivivax benzoatilyticus
Appl. Microbiol. Biotechnol.
89
1001-1008
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
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1
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1
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1
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-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
672116
Ralph
Mechanistic studies of the fla ...
Pseudomonas savastanoi
Biochemistry
45
15844-15852
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
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1
-
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-
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1
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1
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1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654682
Sobrado
Analysis of the role of the ac ...
Pseudomonas savastanoi
Biochemistry
42
13826-13832
2003
-
-
-
-
2
-
2
6
-
-
-
-
-
1
-
-
-
-
-
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1
-
-
-
-
3
-
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6
-
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-
2
-
-
2
6
6
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
654683
Sobrado
Identification of Tyr413 as an ...
Pseudomonas savastanoi
Biochemistry
42
13833-13838
2003
-
-
-
-
2
-
2
6
-
-
-
-
-
1
-
-
-
-
-
-
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1
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3
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6
-
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2
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2
6
6
-
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1
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-
3
-
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-
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-
-
-
-
439346
Sobrado
Analysis of the roles of amino ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
402
24-30
2002
-
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3
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2
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1
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1
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3
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439349
Lemcke
The ORF8 gene product of Agrob ...
Agrobacterium rhizogenes
Mol. Plant Microbe Interact.
13
787-790
2000
-
-
1
-
-
-
-
-
-
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4
-
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1
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1
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1
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-
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439348
Gadda
Characterization of 2-oxo-3-pe ...
Pseudomonas savastanoi
Biochemistry
38
5822-5828
1999
-
-
1
-
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1
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-
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1
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1
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1
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439347
Emanuele
Purification and characterizat ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
316
241-248
1995
-
-
1
-
-
-
16
7
-
-
-
1
-
4
-
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1
-
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1
2
7
-
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1
-
7
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1
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1
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1
1
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16
-
7
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1
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1
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1
2
7
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1
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7
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1
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-
-
-
-
439343
Hutcheson
Regulation of 3-indoleacetic a ...
Pseudomonas savastanoi
J. Biol. Chem.
260
6281-6287
1985
-
-
-
-
-
1
2
1
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2
1
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4
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1
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1
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1
1
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1
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1
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1
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439344
Yamada
Nucleotide sequences of the Ps ...
Agrobacterium tumefaciens, Pseudomonas savastanoi
Proc. Natl. Acad. Sci. USA
82
6522-6526
1985
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1
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5
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439345
Comai
Cloning characterization of ia ...
Pseudomonas savastanoi
J. Bacteriol.
149
40-46
1982
-
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1
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1
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3
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1
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439342
Comai
Involvement of plasmid deoxyri ...
Pseudomonas savastanoi
J. Bacteriol.
143
950-957
1980
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1
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1
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439341
Kosuge
Microbial synthesis and degrad ...
Pseudomonas savastanoi
J. Biol. Chem.
241
3738-3744
1966
-
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19
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1
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1
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1
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1
2
5
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1
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1
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1
1
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19
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1
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1
2
5
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1
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