BRENDA - Enzyme Database show
show all sequences of 1.13.12.3

Regulation of 3-indoleacetic acid production in Pseudomonas syringae pv. savastanoi. Purification and properties of tryptophan 2-monooxygenase

Hutcheson, S.W.; Kosuge, T.; J. Biol. Chem. 260, 6281-6287 (1985)

Data extracted from this reference:

General Stability
General Stability
Organism
FAD has no effect on stability
Pseudomonas savastanoi
Inhibitors
Inhibitors
Commentary
Organism
Structure
Indole-3-acetamide
end product inhibition
Pseudomonas savastanoi
indole-3-acetic acid
-
Pseudomonas savastanoi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
L-tryptophan
-
Pseudomonas savastanoi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
amino acid composition
Pseudomonas savastanoi
85000
-
gel filtration
Pseudomonas savastanoi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Pseudomonas savastanoi
-
indole-3-acetamide + CO2 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas savastanoi
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas savastanoi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.28
-
-
Pseudomonas savastanoi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
439343
Pseudomonas savastanoi
indole-3-acetamide + CO2 + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 62000, SDS-PAGE
Pseudomonas savastanoi
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains 0.73 FAD molecules per subunit, addition of FAD to partially purified preparation has no effect on activity
Pseudomonas savastanoi
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains 0.73 FAD molecules per subunit, addition of FAD to partially purified preparation has no effect on activity
Pseudomonas savastanoi
General Stability (protein specific)
General Stability
Organism
FAD has no effect on stability
Pseudomonas savastanoi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Indole-3-acetamide
end product inhibition
Pseudomonas savastanoi
indole-3-acetic acid
-
Pseudomonas savastanoi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
L-tryptophan
-
Pseudomonas savastanoi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
amino acid composition
Pseudomonas savastanoi
85000
-
gel filtration
Pseudomonas savastanoi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Pseudomonas savastanoi
-
indole-3-acetamide + CO2 + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas savastanoi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.28
-
-
Pseudomonas savastanoi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
439343
Pseudomonas savastanoi
indole-3-acetamide + CO2 + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 62000, SDS-PAGE
Pseudomonas savastanoi
Other publictions for EC 1.13.12.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746273
Zhang
Regulation of oncogene expres ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
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1-27
2015
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1
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2
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-
1
2
2
1
-
-
745813
Yin
Characterization of a tryptop ...
Puccinia graminis f. sp. tritici, Puccinia graminis f. sp. tritici CRL 75-36-700-3
Mol. Plant Microbe Interact.
27
227-235
2014
-
-
1
-
-
-
-
-
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2
-
10
-
-
-
-
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2
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1
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2
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2
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2
2
-
-
-
746257
Mujahid
Aniline is an inducer, and no ...
Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2
PLoS ONE
9
e87503
2014
-
-
1
-
-
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-
-
-
-
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2
-
7
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2
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1
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1
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1
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-
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-
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-
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-
2
-
-
-
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-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
727017
Gaweska
Structure of the flavoprotein ...
Pseudomonas savastanoi
Biochemistry
52
2620-2626
2013
-
-
1
1
3
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
5
5
726752
Mujahid
Production of indole-3-acetic ...
Rubrivivax benzoatilyticus
Appl. Microbiol. Biotechnol.
89
1001-1008
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
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1
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1
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1
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1
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1
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-
1
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-
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-
1
1
-
-
-
672116
Ralph
Mechanistic studies of the fla ...
Pseudomonas savastanoi
Biochemistry
45
15844-15852
2006
-
-
-
-
-
-
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1
-
2
-
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1
-
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2
-
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-
-
1
-
-
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-
-
-
1
-
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1
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1
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2
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-
654682
Sobrado
Analysis of the role of the ac ...
Pseudomonas savastanoi
Biochemistry
42
13826-13832
2003
-
-
-
-
2
-
2
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
2
-
-
2
6
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
654683
Sobrado
Identification of Tyr413 as an ...
Pseudomonas savastanoi
Biochemistry
42
13833-13838
2003
-
-
-
-
2
-
2
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
2
-
-
2
6
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
439346
Sobrado
Analysis of the roles of amino ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
402
24-30
2002
-
-
-
-
3
-
-
-
-
-
-
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2
-
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-
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-
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1
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1
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3
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-
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-
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-
-
-
-
-
-
-
-
-
439349
Lemcke
The ORF8 gene product of Agrob ...
Agrobacterium rhizogenes
Mol. Plant Microbe Interact.
13
787-790
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
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-
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-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439348
Gadda
Characterization of 2-oxo-3-pe ...
Pseudomonas savastanoi
Biochemistry
38
5822-5828
1999
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
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-
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1
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1
1
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1
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-
1
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-
-
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-
-
-
-
439347
Emanuele
Purification and characterizat ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
316
241-248
1995
-
-
1
-
-
-
16
7
-
-
-
1
-
4
-
-
1
-
-
-
1
2
7
-
-
1
-
7
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
16
-
7
-
-
-
1
-
-
-
1
-
-
1
2
7
-
-
1
-
7
-
1
-
-
-
-
-
-
-
-
439343
Hutcheson
Regulation of 3-indoleacetic a ...
Pseudomonas savastanoi
J. Biol. Chem.
260
6281-6287
1985
-
-
-
-
-
1
2
1
-
-
2
1
-
4
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
1
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439344
Yamada
Nucleotide sequences of the Ps ...
Agrobacterium tumefaciens, Pseudomonas savastanoi
Proc. Natl. Acad. Sci. USA
82
6522-6526
1985
-
-
-
-
-
-
-
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1
2
-
5
-
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2
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1
2
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2
-
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-
-
-
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-
-
-
-
-
-
-
-
439345
Comai
Cloning characterization of ia ...
Pseudomonas savastanoi
J. Bacteriol.
149
40-46
1982
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
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1
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1
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1
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-
439342
Comai
Involvement of plasmid deoxyri ...
Pseudomonas savastanoi
J. Bacteriol.
143
950-957
1980
-
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1
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1
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3
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2
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1
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1
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2
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-
439341
Kosuge
Microbial synthesis and degrad ...
Pseudomonas savastanoi
J. Biol. Chem.
241
3738-3744
1966
-
-
-
-
-
-
19
-
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1
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1
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1
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1
2
5
-
1
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1
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1
1
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19
-
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1
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1
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1
2
5
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1
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1
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1
1
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