BRENDA - Enzyme Database show
show all sequences of 1.13.12.3

Characterization of a tryptophan 2-monooxygenase gene from Puccinia graminis f. sp. tritici involved in auxin biosynthesis and rust pathogenicity

Yin, C.; Park, J.; Gang, D.; Hulbert, S.; Mol. Plant Microbe Interact. 27, 227-235 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene PGTG_11658 or Pgt-IaaM, transgenic expression of gene Pgt-IaaM in Arabidopsis thaliana haustoria causes a typical auxin expression phenotype and promotes susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000, transgenic expression of gene Pgt-IaaM in Triticum aestivum leaves, phenotypes, overview. Silencing of Pgt-IaaM by wheat host-induced gene silencing reduces the pathogenicity of Puccinia graminis. Expression of Pgt-IaaM in Arabidopsis thaliana displays pleiotropic auxin-related phenotypes
Puccinia graminis f. sp. tritici
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Puccinia graminis f. sp. tritici
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
L-tryptophan + O2
Puccinia graminis f. sp. tritici CRL 75-36-700-3
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Puccinia graminis f. sp. tritici
E3KNM7
-
-
Puccinia graminis f. sp. tritici CRL 75-36-700-3
E3KNM7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
745813
Puccinia graminis f. sp. tritici
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
745813
Puccinia graminis f. sp. tritici CRL 75-36-700-3
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PGTG_11658 or Pgt-IaaM, transgenic expression of gene Pgt-IaaM in Arabidopsis thaliana haustoria causes a typical auxin expression phenotype and promotes susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000, transgenic expression of gene Pgt-IaaM in Triticum aestivum leaves, phenotypes, overview. Silencing of Pgt-IaaM by wheat host-induced gene silencing reduces the pathogenicity of Puccinia graminis. Expression of Pgt-IaaM in Arabidopsis thaliana displays pleiotropic auxin-related phenotypes
Puccinia graminis f. sp. tritici
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Puccinia graminis f. sp. tritici
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
L-tryptophan + O2
Puccinia graminis f. sp. tritici CRL 75-36-700-3
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
745813
Puccinia graminis f. sp. tritici
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
745813
Puccinia graminis f. sp. tritici CRL 75-36-700-3
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
transient silencing of the tryptophan 2-monooxygenase-encoding gene iaaM in infected wheat plants indicates that it is required for full pathogenicity
Puccinia graminis f. sp. tritici
physiological function
tryptophan 2-monooxygenase from Puccinia graminis f. sp. tritici produces the auxin precursor indole-3-acetamide (IAM) and is involved in auxin biosynthesis and rust pathogenicity. Auxin acts as a regulator of plant growth and development but its production can also affect plant–microbe interactions. Expression of Pgt-IaaM in Arabidopsis thaliana haustoria causes a typical auxin expression phenotype and promotes susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000
Puccinia graminis f. sp. tritici
General Information (protein specific)
General Information
Commentary
Organism
malfunction
transient silencing of the tryptophan 2-monooxygenase-encoding gene iaaM in infected wheat plants indicates that it is required for full pathogenicity
Puccinia graminis f. sp. tritici
physiological function
tryptophan 2-monooxygenase from Puccinia graminis f. sp. tritici produces the auxin precursor indole-3-acetamide (IAM) and is involved in auxin biosynthesis and rust pathogenicity. Auxin acts as a regulator of plant growth and development but its production can also affect plant–microbe interactions. Expression of Pgt-IaaM in Arabidopsis thaliana haustoria causes a typical auxin expression phenotype and promotes susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000
Puccinia graminis f. sp. tritici
Other publictions for EC 1.13.12.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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727017
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Pseudomonas savastanoi
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52
2620-2626
2013
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5
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1
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5
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726752
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89
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Pseudomonas savastanoi
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42
13826-13832
2003
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2
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2
6
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1
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3
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6
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6
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1
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654683
Sobrado
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Pseudomonas savastanoi
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42
13833-13838
2003
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2
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2
6
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24-30
2002
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3
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439349
Lemcke
The ORF8 gene product of Agrob ...
Agrobacterium rhizogenes
Mol. Plant Microbe Interact.
13
787-790
2000
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1
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4
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439348
Gadda
Characterization of 2-oxo-3-pe ...
Pseudomonas savastanoi
Biochemistry
38
5822-5828
1999
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1
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1
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439347
Emanuele
Purification and characterizat ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
316
241-248
1995
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1
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16
7
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7
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16
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7
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7
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7
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439343
Hutcheson
Regulation of 3-indoleacetic a ...
Pseudomonas savastanoi
J. Biol. Chem.
260
6281-6287
1985
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439344
Yamada
Nucleotide sequences of the Ps ...
Agrobacterium tumefaciens, Pseudomonas savastanoi
Proc. Natl. Acad. Sci. USA
82
6522-6526
1985
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439345
Comai
Cloning characterization of ia ...
Pseudomonas savastanoi
J. Bacteriol.
149
40-46
1982
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439342
Comai
Involvement of plasmid deoxyri ...
Pseudomonas savastanoi
J. Bacteriol.
143
950-957
1980
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439341
Kosuge
Microbial synthesis and degrad ...
Pseudomonas savastanoi
J. Biol. Chem.
241
3738-3744
1966
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